Artículo

Aronzon, C.M.; Sandoval, M.T.; Herkovits, J.; Pérezcoll, C.S. "Stage-dependent susceptibility to copper in Rhinella arenarum embryos and larvae" (2011) Environmental Toxicology and Chemistry. 30(12):2771-2777
Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Copper toxicity in different embryonic and larval stages of the common South American toad Rhinella arenarum was evaluated by means of continuous and 24-h pulse treatments in 12 different developmental stages. Lethal concentrations (LC) of 10, 50, and 90% of continuous treatment with Cu from early blastula (S.4), complete operculum (S.25), and hind limb bud (S.28) stages were plotted from 24 to 168h, resulting from S.4 in a 24-h LC50 of 137μg Cu 2+/L and a 168-h LC50 of 19.5μg Cu 2+/L. This result was in agreement with pulse treatments that showed a high resistance to Cu at blastula and gastrula stages, whereas the organogenic period, between muscular response (S.18) and open mouth (S.21), was very susceptible to this metal. Continuous treatments from S.25 showed no significant differences along exposure time (168-h LC50=51μg Cu 2+/L), but in the case of S.28 toxicity increased slightly from a 24-h LC50 of 138.6μg Cu 2+/L to a 168-h LC50 of 104μg Cu 2+/L, pointing out that, although the larval period was significantly more resistant to Cu, there was also a remarkable stage-dependent susceptibility to this metal. Copper teratogenic potential was approximately two, and main adverse effects were reduced body size, axial flexure, microcephaly, acephaly, mouth malformations, agenesis of or underdeveloped gills, agenesis of or underdeveloped tail, and hydropsy. The results are discussed considering Cu toxicity mechanisms, an evolutionary perspective, and environmental protection. © 2011 SETAC.

Registro:

Documento: Artículo
Título:Stage-dependent susceptibility to copper in Rhinella arenarum embryos and larvae
Autor:Aronzon, C.M.; Sandoval, M.T.; Herkovits, J.; Pérezcoll, C.S.
Filiación:Programa de Seguridad Química, Instituto de Ciencias Ambientales y Salud (ICAS), Fundación PROSAMA, Buenos Aires, Argentina
Laboratorio de Herpetología, Facultad de Ciencias Exactas y Naturales y Agrimensura, Universidad Nacional del Nordeste, Corrientes, Argentina
Instituto de Investigación e Ingeniería Ambiental, Universidad Nacional de San Martín, Buenos Aires, Argentina
Idioma: Inglés
Palabras clave:Amphibian embryos; AMPHITOX; Copper; Stage-dependent toxicity; Teratogenesis; Adverse effect; Amphibian embryos; AMPHITOX; Body sizes; Copper toxicity; Developmental stage; Evolutionary perspective; Exposure-time; Gastrula stage; High resistance; Hind limbs; Larval periods; Larval stage; Lethal concentration; Teratogenesis; Toxicity; Copper; copper; concentration (composition); copper; embryo; environmental protection; frog; ion; larva; toxicity; agenesis; animal embryo; animal experiment; article; blastula; body size; developmental stage; environmental protection; evoked muscle response; evolutionary developmental biology; female; gastrula; gill; larva; LC 50; limb bud; microcephaly; mouth; mouth malformation; nonhuman; organogenesis; parietal operculum; priority journal; Rhinella arenarum; South America; tail; teratogenesis; toad; Animals; Bufo arenarum; Copper; Embryo, Nonmammalian; Gills; Larva; Lethal Dose 50; Teratogens; Water Pollutants, Chemical; South America; Amphibia; Bufo americanus
Año:2011
Volumen:30
Número:12
Página de inicio:2771
Página de fin:2777
DOI: http://dx.doi.org/10.1002/etc.674
Título revista:Environmental Toxicology and Chemistry
Título revista abreviado:Environ. Toxicol. Chem.
ISSN:07307268
CODEN:ETOCD
CAS:copper, 15158-11-9, 7440-50-8; Copper, 7440-50-8; Teratogens; Water Pollutants, Chemical
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07307268_v30_n12_p2771_Aronzon

Referencias:

  • O'Dell, B.L., Copper (1990) Present Knowledge in Nutrition, pp. 261-267. , Brown M, ed, 6th ed. International Life Sciences Institute Nutrition Foundation, Washington, DC, USA
  • (1998), World Health Organization. Environmental Health Criteria 200: Copper The International Programme on Chemical Safety, Geneva, SwitzerlandHandy, R.D., Chronic effects of copper exposure versus endocrine toxicity: Two sides of the same toxicological process? (2003) Comp Biochem Physiol A Mol Integr Physiol, 135, pp. 25-38
  • Wake, D.B., Vredenburg, V.T., Are we in the midst of the sixth mass extinction? A view from the world of amphibians (2008) Proc Natl Acad Sci U S A, 105, pp. 11466-11473
  • van der Schalie, W.H., Gardner Jr., H.S., Bantle, J.A., De Rosa, C.T., Finch, R.A., Reif, J.S., Reuter, R.H., Stokes, W.S., Animals as sentinels of human health hazards of environmental chemicals (1999) Environ Health Perspect, 107, pp. 309-315
  • Pérez-Coll, C.S., Sztrum, A.A., Herkovits, J., Nickel tissue residue as a biomarker of sub-toxic exposure and susceptibility in amphibian embryos (2008) Chemosphere, 74, pp. 78-83
  • Pérez-Coll, C.S., Herkovits, J., Fridman, O., Daniel, P., D'Eramo, J.L., Metallothionein induction and cadmium uptake in Bufo arenarum embryos following an acclimation protocol (1999) Environ Pollut, 106, pp. 443-448
  • Mann, R.M., Hyne, R.V., Choung, C.B., Wilson, S.P., Amphibians and agricultural chemicals: Review of the risks in a complex environment (2009) Environ Pollut, 157, pp. 2903-2927
  • Brodeur, J.C., Svartz, G., Perez-Coll, C.S., Marino, D.J.G., Herkovits, J., Comparative susceptibility to atrazine of three developmental stages of Rhinella arenarum and influence on metamorphosis: Non-monotonous acceleration of the time to climax and delayed tail resorption (2009) Aquat Toxicol, 91, pp. 161-170
  • Mann, R.M., Bidwell, J.R., Application of the FETAX protocol to assess the developmental toxicity of nonylphenol ethoxylate to Xenopus laevis and two Australian frogs (2000) Aquat Toxicol, 51, pp. 19-29
  • Pérez-Coll, C.S., Herkovits, J., Lethal and teratogenic effects of naringenin evaluated by means of an amphibian embryo toxicity test (AMPHITOX) (2004) Food Chem Toxicol, 42, pp. 299-306
  • Castañaga, L.A., Asorey, C.M., Sandoval, M.T., Perez-Coll, C.S., Argibay, T.I., Herkovits, J., Stage dependent teratogenic and lethal effects exerted by ultraviolet B radiation on Rhinella (Bufo) arenarum embryos (2009) Environ Toxicol Chem, 28, pp. 427-433
  • Bancroft, B.A., Baker, N.J., Blaustein, A.R., A meta-analysis of the effects of ultraviolet B radiation and its synergistic interactions with pH, contaminants, and disease on amphibian survival (2007) Conserv Biol, 22, pp. 987-996
  • Ferreira, C.M., Lombardi, J.V., Machado-Neto, J.G., Bueno-Guimarães, H.M., Soares, S.R., Saldiva, P.H., Effects of copper oxychloride in Rana catesbeiana tadpoles: Toxicological and bioaccumulative aspects (2004) Bull Environ Contam Toxicol, 73, pp. 465-470
  • Bridges, C.M., Dwyer, F.J., Hardesty, D.K., Whites, D.W., Comparative contaminant toxicity: Are amphibian larvae more sensitive than fish? (2002) Bull Environ Contam Toxicol, 69, pp. 562-569
  • Chen, T., Gross, J.A., Karasov, W.H., Adverse effects of chronic copper exposure in larval northern leopard frogs (Rana pipiens) (2007) Environ Toxicol Chem, 26, pp. 1470-1475
  • Redick, M.S., La Point, T.W., Effects of sublethal copper exposure on behavior and growth of Rana pipiens tadpoles (2004) Bull Environ Contam Toxicol, 72, pp. 706-710
  • Pérez-Coll, C.S., Herkovits, J., Stage dependent susceptibility to lead in Bufo arenarum embryos (1990) Environ Pollut, 63, pp. 239-245
  • Pérez-Coll, C.S., Herkovits, J., Stage-dependent uptake of cadmium by Bufo arenarum embryos (1996) Bull Environ Contam Toxicol, 56, pp. 663-669
  • Sztrum, A.A., D'Eramo, J.L., Herkovits, J., Nickel toxicity in embryos and larvae of the South American toad: Effects on cell differentiation, morphogenesis, and oxygen consumption (2011) Environ Toxicol Chem, 30, pp. 1146-1152
  • Del Conte, E., Sirlin, L., The firts stages of Bufo arenarum development (1951) Acta Zool Lilloana, 12, pp. 495-499
  • Echeverria, D.D., Fiorito de López, L.E., Estadios de la metamorfosis en Bufo arenarum (Anura) (1981) Biol Acuat, 1, pp. 15-23
  • (1988) Users Guide for a Computer Program for PROBIT Analysis of Data from Acute and Short-Term Chronic Toxicity Test with Aquatic Organisms, , U.S. Environmental Protection Agency. Biological Methods, Environmental Monitoring and Support, Laboratory, Cincinnati, OH, USA
  • Herkovits, J., Helguero, L.A., Copper toxicity and copper-zinc interactions in amphibian embryos (1998) Sci Total Environ, 221, pp. 1-10
  • (1980) Standard Methods for the Examination of Water and Wastewater, , American Public Health Association. American Water Works Association, Water Pollution Control, Federation, and American Public Health Association, Washington, DC
  • Aronzon, C., Sandoval, M., Herkovits, J., Pérez-Coll, C., Stage-dependent toxicity of 2,4-dichlorophenoxyacetic on the embryonic development of a South American toad, Rhinella arenarum (2011) Environ Toxicol, 26, pp. 373-381
  • Edginton, A., Rouleau, C., Stephenson, G., Boermans, H., 2,4-D butoxyethyl ester kinetics in embryos of Xenopus laevis: The role of the embryonic jelly coat in reducing chemical absorption (2007) Arch Environ Contam Toxicol, 52, pp. 113-120
  • Herkovits, J., Pérez-Coll, C., Evaluation of nickel-zinc interactions by means of bioassays with amphibian embryos (2000) Ecotoxicol Environ Saf, 45, pp. 266-273
  • Santore, R.C., Di Toro, D.M., Paquin, P.R., Allen, H.E., Meyer, J.S., Biotic ligand model on the acute toxicity of metals. 2. Application to acute copper toxicity in freshwater fish and Daphnia (2001) Environ Toxicol Chem, 20, pp. 2397-2402
  • Herkovits, J., Pérez-Coll, C.S., A customized set of toxicity tests employing amphibian embryos (2003) Multiple Stressor Effects in Relation to Declining Amphibian Populations, pp. 46-60. , Linder GL, Krest S, Sparling D, Little EE, eds, STP 1443. American Society for Testing and Materials, West Conshohocken, PA
  • Herkovits, J., Herkovits, F.D., Pérez-Coll, C., Identification of aluminium toxicity and aluminium-zinc interaction in amphibian Bufo arenarum embryos (1997) Environ Sci, 5, pp. 57-64
  • Standard guide for conducting the frog embryo teratogenesis assay-Xenopus (FETAX) (1993) Standards on Aquatic Toxicology and Hazard Evaluation, pp. 1199-1209. , Amercian Society for Testing and Materials. In. Philadelphia, PA
  • Herkovits, J., Shape regulation capacity during development: recovery of embryos developing notwithstanding asymmetry until the neurula stage (1977) Acta Embryol Exp, 1, pp. 3-10
  • Saxen, L., Mechanism of teratogenesis (1976) J Embryol Exp Morphol, 36, pp. 1-12
  • Valko, M., Rhodes, C.J., Moncola, J., Izakovic, M., Mazura, M., Free radicals, metals and antioxidants in oxidative stress-induced cancer (2006) Chem Biol Interact, 160, pp. 1-40
  • Grosell, M., Wood, C.M., Copper uptake across rainbow trout gills: mechanisms of apical entry (2002) J Exp Biol, 205, pp. 1179-1188
  • Vieira, L.R., Gravato, C., Soares, A.M.V.M., Morgado, F., Guilhermino, L., Acute effects of copper and mercury on the estuarine fish Pomatoschistus microps: Linking biomarkers to behaviour (2009) Chemosphere, 76, pp. 1416-1427
  • Herkovits, J., Evoecotoxicology: Environmental changes and life features development during the evolutionary process-The record of the past at developmental stages of living organisms (2006) Environ Health Perspect, 114, pp. 1139-1142
  • Konhauser, K., Pecoits, E., Lalonde, S., Papineau, D., Nisbet, E., Barley, M., Arndt, N., Kamber, B., Oceanic nickel depletion and a methanogen famine before the great oxidation event (2009) Nature, 458, pp. 750-753
  • Ferrari, L., de la Torre, F.R., Demichelis, S.O., García, M.E., Salibián, A., Ecotoxicological assessment for receiving waters with the premetamorphic tadpoles acute assay (2005) Chemosphere, 59, pp. 567-575

Citas:

---------- APA ----------
Aronzon, C.M., Sandoval, M.T., Herkovits, J. & Pérezcoll, C.S. (2011) . Stage-dependent susceptibility to copper in Rhinella arenarum embryos and larvae. Environmental Toxicology and Chemistry, 30(12), 2771-2777.
http://dx.doi.org/10.1002/etc.674
---------- CHICAGO ----------
Aronzon, C.M., Sandoval, M.T., Herkovits, J., Pérezcoll, C.S. "Stage-dependent susceptibility to copper in Rhinella arenarum embryos and larvae" . Environmental Toxicology and Chemistry 30, no. 12 (2011) : 2771-2777.
http://dx.doi.org/10.1002/etc.674
---------- MLA ----------
Aronzon, C.M., Sandoval, M.T., Herkovits, J., Pérezcoll, C.S. "Stage-dependent susceptibility to copper in Rhinella arenarum embryos and larvae" . Environmental Toxicology and Chemistry, vol. 30, no. 12, 2011, pp. 2771-2777.
http://dx.doi.org/10.1002/etc.674
---------- VANCOUVER ----------
Aronzon, C.M., Sandoval, M.T., Herkovits, J., Pérezcoll, C.S. Stage-dependent susceptibility to copper in Rhinella arenarum embryos and larvae. Environ. Toxicol. Chem. 2011;30(12):2771-2777.
http://dx.doi.org/10.1002/etc.674