Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Renal 11 β-hydroxysteroid dehydrogenase 2 (HSD2) catalyzes the conversion of active glucocorticoids to inert 11 β-keto compounds, thereby preventing the illicit binding of these hormones to mineralocorticoid receptors (MRs) and, thus, conferring aldosterone specificity. Absence or inhibition of HSD2 activity, originates a hypertensive syndrome with sodium retention and increased potassium elimination. Recent studies from our laboratory reported an increment of HSD2 activity in intact-stressed rats. To evaluate the adrenal involvement in this increase, we analyzed HSD2 activity and protein abundance in Intact, Sham-operated, and adrenalectomized rats under stress situations (gavage with an overload of 200 mM HCl (10 ml) and simulated gavage) or with corticosterone replacement. HSD2 activity was assessed in renal microsomal preparations obtained from different groups of animals. HSD2 protein abundance was measured by Western-blot. Circulating corticosterone was determined by radioimmunoassay. Sham-operated animals showed an increase in HSD2 activity and abundance compared to Intact and adrenalectomized rats suggesting the involvement of stress-related adrenal factors in HSD2 regulation. In the case of acidotic adrenalectomized animals, there was an increase in renal HSD2 activity when, along with the HCl overload, the rats were injected with corticosterone. This increment occurred without an increase in enzyme abundance. These results suggest the importance of circulating levels of glucocorticoids to respond to a metabolic acidosis, through regulation of HSD2 stimulation. The group subjected to a simulated gavage showed an increase in enzyme activity and protein abundance, thus demonstrating the need for both adrenal and extra-factors in the modulation of renal HSD2. The adrenalectomized animals injected with different doses of corticosterone, produced a progressive increase in enzyme activity and abundance, being significant for the dose of 68 ng corticosterone/100 g body weight. The highest dose (308 ng/100 g body weight) did not show any variation in activity and abundance compared to the control group. This biphasic effect of glucocorticoids could be explained taking into account their permissive and suppressive actions, depending on their blood levels. Knowing that stress induces multifactorial responses, it should not be suprising to observe a differential regulation in renal HSD2, confirming that different Stressors act through different factors of both, adrenal and extra-adrenal origin. © 2004 Wiley-Liss, Inc.

Registro:

Documento: Artículo
Título:Adrenal gland involvement in the regulation of renal 11 β-hydroxysteroid dehydrogenase 2
Autor:Zallocchi, M.L.; Matkovic, L.; Calvo, J.C.; Damasco, M.C.
Filiación:Department of Biological Chemistry, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires and PRHOM-CONICET, Buenos Aires, Argentina
Instituto de Biología y Medicina Experimental (CONICET), Buenos Aires, Argentina
Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria-Núnez, (1428) Buenos Aires, Argentina
Palabras clave:Acidosis; Corticosterone; HSD2; Kidney; Stress; 11beta hydroxysteroid dehydrogenase; chloride; corticosterone; glucocorticoid; corticosterone; adrenal gland; adrenalectomy; animal experiment; animal model; article; body weight; controlled study; enzyme activity; enzyme regulation; feeding; metabolic acidosis; nonhuman; priority journal; radioimmunoassay; rat; stress; Western blotting; adrenal gland; animal; blood; enzymology; gene expression regulation; kidney; kinetics; metabolism; physiology; Animalia; 11-beta-Hydroxysteroid Dehydrogenase Type 2; Adrenal Glands; Adrenalectomy; Animals; Corticosterone; Gene Expression Regulation, Enzymologic; Kidney; Kinetics; Models, Animal; Rats
Año:2004
Volumen:92
Número:3
Página de inicio:591
Página de fin:602
DOI: http://dx.doi.org/10.1002/jcb.20078
Título revista:Journal of Cellular Biochemistry
Título revista abreviado:J. Cell. Biochem.
ISSN:07302312
CODEN:JCEBD
CAS:11beta hydroxysteroid dehydrogenase, 9041-46-7; chloride, 16887-00-6; corticosterone, 50-22-6; 11-beta-Hydroxysteroid Dehydrogenase Type 2, EC 1.1.1.146; Corticosterone, 50-22-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07302312_v92_n3_p591_Zallocchi

Referencias:

  • Agarwal, A., Expression of HSDIIK (NAD+dependent IIbeta-hydroxysteroid dehydrogenase) promoter constructs in renal cell lines (2000) Endocr Res, 26, pp. 289-302
  • Akana, S., Dallman, M., Chronic cold in adrenalectomized, corticosterone (B)-treated rats: Facilitated corticotropin responses to acute restraint emerge as B increases (1997) Endocrinology, 138, pp. 3249-3258
  • Alfaidy, N., Blot-Chabaud, M., Robic, D., Kenouch, S., Bourbouze, R., Bonvalet, J., Farman, N., Characteristics and regulation of 11 beta-hydroxysteroid dehydrogenase of proximal and distal nephron (1995) Biochem Bioph Acta, 1243, pp. 461-468
  • Alfaidy, N., Blot-Chabaud, M., Bonvalet, J., Farman, N., Vasopressin potentiates mineralocorticoid selectivity by stimulating 11β-hydroxysteroid dehydrogenase in rat collecting duct (1997) J Clin Invest, 100, pp. 2437-2442
  • Arriza, J., Weinberger, C., Cerelli, G., Glaser, T., Handelin, B., Housman, D., Evans, R., Cloning of human mineralocorticoid receptor complementary DNA: Structural and functional kinship with the glucocorticoid receptor (1987) Science, 237, pp. 268-275
  • Audigé, A., Dick, B., Frey, B., Frey, M., Corman, B., Vogt, B., Glucocorticoids and 11β-hydroxysteroid dehydrogenase type 2 gene expression in the aging kidney (2002) Eur J Clin Invest, 32, pp. 411-420
  • Bonvalet, J., Doignon, I., Blot-Chabaud, M., Pradelles, P., Farman, N., Distribution of 11β-hydroxysteroid dehydrogenase along the rabbit nephron (1990) J Clin Invest, 86, pp. 832-837
  • Bradford, M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding (1976) Ann Biochem, 72, pp. 248-254
  • Brooks, H.L., Ageloff, S., Kwon, T.H., Brandt, W., Terris, J.M., Seth, A., Michea, L., Knepper, M.A., DNA array identification of genes regulated in rat renal medulla in response to vasopressin infusion (2003) Am J Physiol Renal Physiol, 284, pp. F218-F228
  • Carlton, B.D., Barlett, P., Basaran, A., Colling, K., Osis, I., Smith, M.K., Reproductive effects of alternative disinfectants (1986) Environ Health Perspect, 69, pp. 237-241
  • Chen, K., Suh, J., Carr, A.C., Morrow, J.D., Zeind, J., Frei, B., Vitamin C suppresses oxidative lipid damage in vivo, even in the presence of iron overload (2000) Am J Physiol Endocrinol Metab, 279, pp. E1406-E1412
  • Dallman, M., Akana, S., Cascio, C., Darlington, D., Jacobson, L., Levin, N., Regulation of ACTH secretion: Variations on a theme of B (1987) Recent Prog Horm Res, 43, pp. 113-173
  • Damasco, M.C., Coronel De Pedazzini, M., Corticosterone secretion by rats after adrenalectomy (1985) Acta Physiol Pharmacol Latin Am, 35, pp. 415-417
  • Damasco, M.C., Díaz, F., Cenal, J., Lantos, C., Acute effects of three natural corticosteroids on the acid-base and electrolyte composition of urine in adrenalectomized rats (1979) Acta Physiol Latinoam, 29, pp. 305-314
  • Damasco, M.C., Ansaldo, M., Malnic, G., Effects of adrenalectomy and acute replacement by corticosteroids on distal acidification (1990) Can J Physiol Pharmacol, 67, pp. 607-614
  • Darnel, A., Archer, T., Yang, K., Regulation of 11β-hydroxysteroid dehydrogenase type 2 by steroid hormones and epidermal growth factor in the Ishikawa human endometrial cell line (1999) J Steroid Biochem Mol Biol, 70, pp. 203-210
  • Diederich, S., Quinkler, M., Miller, K., Heilmann, P., Schoneshofer, M., Oelkers, W., Human kidney 11 beta-hydroxysteroid dehydrogenase: Regulation by adrenocorticotropin? (1996) Eur J Endocrinol, 134, pp. 301-307
  • Edwards, C., Stewart, P., Burt, D., Brett, L., McIntyre, M., Sutanto, W., De Kloet, E., Monder, C., Localisation of 11 beta-hydroxysteroid dehydrogenase-tissue specific protector of the mineralocorticoid receptor (1988) Lancet, 2, pp. 986-989
  • Farman, N., Bocchi, B., Mineralocorticoid selectivity: Molecular and cellular aspects (2000) Kidney Int, 57, pp. 1364-1369
  • Ferrari, P., Krozowski, Z., Role of the 11β-hydroxysteroid dehydrogenase type 2 in blood pressure regulation (2000) Kidney Int, 57, pp. 1374-1381
  • Ferrari, P., Obeyesekere, V., Li, K.X.Z., Aragona, F., Krozowski, Z., The 11 beta-hydroxysteroid dehydrogenase type II enzyme: Biochemical consequences of the congenital R337C mutation (1996) Steroids, 61, pp. 197-200
  • Funder, J., Pearce, P., Smith, R., Smith, A., Mineralocorticoid action: Target tissue specificity is enzyme, not receptor mediated (1988) Science, 242, pp. 583-585
  • Gomez-Sanchez, E., Gomez-Sanchez, C.E., Central hypertensinogenic effects of glycyrrhizic acid and carbenoxolone (1992) Am J Physiol, 263, pp. E1125-E1130
  • Gomez-Sanchez, C., Murry, B., Kem, D., Kaplan, N., A direct radioimmunoassay of corticosterone in rat serum (1975) Endocrinology, 96, pp. 796-798
  • Gomez-Sanchez, E., Ganjan, V., Chen, Y., Liu, Y., Clark, S., Gomez-Sanchez, C., The 11β-hydroxysteroid dehydrogenase 2 exists as an inactive dimer (2001) Steroids, 66, pp. 845-848
  • Hawk, C.T., Li, L., Schafer, J.A., AVP and aldosterone at physiological concentrations have synergistic effects on Na transport in rat CCD (1996) Kidney Int, 50, pp. S35-S41
  • Hirasawa, G., Takeyama, J., Sasano, H., Fukushima, K., Suzuki, T., Muramatu, Y., Darnel, A., Krozowski, Z., 11β-hydroxysteroid dehydrogenase type II and mineralocorticoid receptor in human placenta (2000) J Clin Endocrinol Metab, 85, pp. 1306-1309
  • Igarreta, P., Calvo, J.C., Paladini, A., Damasco, C., Acute effects of two natural corticosteroids on the kinetics of the Na+/H+ exchanger in rat renal brush-border membranes (1997) Can J Physiol Pharm, 75, pp. 1226-1231
  • Igarreta, P., Lantos, C., Calvo, J.C., Paladini, A., Damasco, C., Dose-dependence of the effects of corticosterone and the non-glucocorticoid 18-hydroxycorticosterone on the brush border Na +/H+ exchanger (1998) Endocr Res, 24, pp. 601-605
  • Igarreta, P., Calvo, J.C., Damasco, C., Activity of renal 11βhydroxysteroid dehydrogenase 2 (11BHSD2) in stressed animals (1999) Life Sci, 64, pp. 2285-2290
  • Jacobson, H., Kokko, J., Intrarenal heterogeneity: Vascular and tubular (1985) The Kidney: Physiology and Pathophysiology, pp. 531-580. , Seldin D, Giebisch G, editors, New York: Raven Press
  • Kotelevtsev, Y., Brown, R., Flemings, Kenyon, C., Edwards, C., Seckl, J., Mullins, J., Hypertension in mice lacking 11β-hydroxysteroid dehydrogenase type 2 (1999) J Clin Invest, 103, pp. 683-689
  • Koyama, K., Krozowski, Z., Modulation of 11 beta-hydroxysteroid dehydrogenase type 2 activity in Ishikawa cells is associated with changes in cellular proliferation (2001) Mol Cell Endocrinol, 183, pp. 165-170
  • Krozowski, Z., The short-chain alcohol dehydrogenase superfamily: Variations on a common theme (1994) J Steroid Biochem Mol Biol, 51, pp. 125-130
  • Krozowski, Z., Funder, J., Renal mineralocorticoid receptors and hippocampal corticosterone binding species have identical intrinsic steroid specificity (1983) Proc Nat Acad Sci USA, 80, pp. 6056-6060
  • Kurokawa, K., Cellular mechanisms and sites of hormone action in the kidney (1985) The Kidney: Physiology and Pathophysiology, pp. 739-774. , Seldin D, Giebisch G, editors. New York: Raven Press
  • Leckie, C., Chapman, K., Edwards, C., Seckl, J., LLC-PK1 cells model 11βhydroxysteroid dehydrogenase type 2 regulation of glueocorticoid access to renal mineralocorticoid receptors (1995) Endocrinology, 136, pp. 5561-5569
  • Li, K.X.Z., Smith, R., Ferrari, P., Funder, J., Krozowski, Z., Rat 11 beta-hydroxysteroid dehydrogenase type 2 enzyme is expressed at low levels in the placenta and is modulated by adrenal steroids in the kidney (1996) Mol Cell Endocrinol, 120, pp. 67-75
  • Mantero, F., Palermo, M., Petrelli, M., Tedde, R., Stewart, P., Shackleton, C., Apparent mineralocorticoid excess: Type I and type II (1996) Steroids, 61, pp. 193-196
  • Meier, J.R., Bull, R.J., Stober, J.A., Cimino, M.C., Evaluation of chemicals used for drinking water disinfection for production of chromosomal damage and sperm-head abnormalities in mice (1985) Environ Mutagen, 7, pp. 201-211
  • Monder, C., Shackleton, C., 11 beta-hydroxysteroid dehydrogenase: Fact or fancy? (1984) Steroids, 44, pp. 383-417
  • Monder, C., Stewart, P., Lakshmi, V., Valentino, R., Burt, D., Edwards, C., Licorice inhibits corticosteroid 11 beta-dehydrogenase of rat kidney and liver: In vivo and in vitro studies (1989) Endocrinology, 5, pp. 1046-1053
  • Munck, A., Náray-Fejes-Tóth, A., The ups and downs of glucocorticoid physiology. Permissive and suppressive effects revisited (1992) Mol Cell Endocrinol, 90, pp. C1-C4
  • Munck, A., Náray-Fejes-Toth, A., Glucocorticoids and stress: Permissive and suppressive actions (1994) Ann NY Acad Sci, 746, pp. 115-130
  • Nawrocki, A.R., Goldring, C.E., Kostadinova, R.M., Frey, F.J., Frey, B.M., In vivo footprinting of the human 11β-hydroxysteroid dehydrogenase type 2 promoter (2002) J Biol Chem, 277, pp. 14647-14656
  • Obeyesekere, V., Ferrari, P., Andrews, R., Wilson, R., New, M., Funder, J., Krozowski, Z., The R337C mutation generates a high Km 11 beta-hydroxysteroid dehydro-genase type II enzyme in a family with apparent mineralocorticoid excess (1995) J Clin Endocrinol Metab, 80, pp. 3381-3383
  • Odermatt, A., Arnold, P., Frey, F., The intracellular localization of the mineralocorticoid receptor is regulated by 11β-hydroxysteroid dehydrogenase type 2 (2001) J Biol Chem, 276, pp. 28484-28492
  • Pacak, K., Palkovits, M., Stressor specificity of control neuroendocrine responses: Implication for stress-related disorders (2001) Endocr Rev, 22, pp. 502-548
  • Sapolsky, R., Romero, M., Munck, A., How do glucocorticoids influence stress responses? Integrating permissive, suppressive, stimulatory, and preparative actions (2000) Endocr Rev, 21, pp. 55-89
  • Seckl, J.R., Walker, B.R., Minireview: 11β-hydroxysteroid dehydrogenase type 1-A tissue-specific amplifier of glucocorticoid action (2001) Endocrinology, 142, pp. 1371-1376
  • Souness, G.W., Morris, D.J., The antinatriuretic and kaliuretic effects of the glucocorticoids corticosterone and cortisol following pretreatment with carbenoxolone sodium (a liquorice derivative) in the adrenalectomized rat (1989) Endocrinology, 124, pp. 1588-1590
  • Stewart, P., Krozowski, Z., 11 beta-hydroxysteroid dehydrogenase (1999) Vitam Horm, 57, pp. 249-324
  • Suzuki, S., Koyama, K., Darnel, A., Ishibashi, H., Kobayashi, S., Kubo, H., Suzuki, T., Krozowski, Z., Dexamethasone up regulates 11{beta}-hydroxysteroid dehydrogenase type 2 in BEAS-2B cells (2003) Am J Respir Crit Care Med, 167, pp. 1244-1249
  • Tanioka, H., Nakamura, K., Fujimura, S., Yoshida, M., Suzuki-Kusaba, M., Hisa, H., Satoh, S., Facilitatory role of NO in neural norepinephrine release in the rat kidney (2002) Am J Physiol: Reg: Integr Comp Physiol, 282, pp. R1436-R1442
  • White, P.C., Mune, T., Agarwal, A.K., 11 beta-hydroxysteroid dehydrogenase and the syndrome of apparent mineralocorticoid excess (1997) Endocr Rev, 18, pp. 135-156
  • Ye, S., Zhong, H., Yanamadala, V., Campese, V., Renal injury caused by intrarenal injection of phenol increases afferent and efferent renal sympathetic nerve activity (2002) Am J Hyper, 15, pp. 717-724

Citas:

---------- APA ----------
Zallocchi, M.L., Matkovic, L., Calvo, J.C. & Damasco, M.C. (2004) . Adrenal gland involvement in the regulation of renal 11 β-hydroxysteroid dehydrogenase 2. Journal of Cellular Biochemistry, 92(3), 591-602.
http://dx.doi.org/10.1002/jcb.20078
---------- CHICAGO ----------
Zallocchi, M.L., Matkovic, L., Calvo, J.C., Damasco, M.C. "Adrenal gland involvement in the regulation of renal 11 β-hydroxysteroid dehydrogenase 2" . Journal of Cellular Biochemistry 92, no. 3 (2004) : 591-602.
http://dx.doi.org/10.1002/jcb.20078
---------- MLA ----------
Zallocchi, M.L., Matkovic, L., Calvo, J.C., Damasco, M.C. "Adrenal gland involvement in the regulation of renal 11 β-hydroxysteroid dehydrogenase 2" . Journal of Cellular Biochemistry, vol. 92, no. 3, 2004, pp. 591-602.
http://dx.doi.org/10.1002/jcb.20078
---------- VANCOUVER ----------
Zallocchi, M.L., Matkovic, L., Calvo, J.C., Damasco, M.C. Adrenal gland involvement in the regulation of renal 11 β-hydroxysteroid dehydrogenase 2. J. Cell. Biochem. 2004;92(3):591-602.
http://dx.doi.org/10.1002/jcb.20078