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Abstract:

The expression of receptors belonging to the epidermal growth factor receptor subfamily has been largely studied these last years in epithelial cells mainly as involved in cell proliferation and malignant progression. Although much work has focused on the role of these growth factor receptors in the differentiation of a variety of tissues, there is little information in regards to normal stromal cells. We investigated erbB2 expression in the murine fibroblast cell line Swiss 3T3L1, which naturally or hormonally induced undergoes adipocyte differentiation. We found that the Swiss 3T3-L1 fibroblasts express erbB2, in addition to EGFR, and in a quantity comparable to or even greater than the breast cancer cell line T47D. Proliferating cells increased erbB2 and EGFR levels when reaching confluence up to 4- and 10-fold, respectively. This expression showed a significant decrease when growth-arrested cells were stimulated to differentiate with dexamethasone and isobutyl-methylxanthine. Differentiated cells presented a decreased expression of both erbB2 and EGFR regardless of whether the cells were hormonally or spontaneously differentiated. EGF stimulation of serum-starved cells increased erbB2 tyrosine phosphorylation and retarded erbB2 migration in SDS-PAGE, suggesting receptor association and activation. Heregulin-α1 and β1, two EGF related factors, had no effect on erbB2 or EGFR phosphorylation. Although 3T3-L1 cells expressed heregulin, its specific receptors, erbB3 and erbB4, were not found. This is the first time in which erbB2 is reported to be expressed in an adipocytic cell line which does not depend on non EGF family growth factors (thyroid hormone, growth hormone, etc.) to accomplish adipose differentiation. Since erbB2 and EGFR expression were downmodulated as differentiation progressed it is conceivable that a mechanism of switching from a mitogenic to a differentiating signaling pathway may be involved, through regulation of the expression of these growth factor receptors. © 2003 Wiley-Liss, Inc.

Registro:

Documento: Artículo
Título:ErbB2 and EGFR are downmodulated during the differentiation of 3T3-L1 preadipocytes
Autor:Pagano, E.; Calvo, J.C.
Filiación:Lab. de Quimica de Proteoglicanos, Inst. de Biol. y Med. Experimental, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Universidad de Buenos Aires, Fac. de Ciencias Exactas y Naturales, Buenos Aires, Argentina
Palabras clave:3T3-L1 cells; Adipocyte differentiation; CAMP; Dexamethasone; EGF; EGFR; ErbB2; Fibroblasts; Heregulin; Insulin; Tyrosine phosphorylation; dexamethasone; epidermal growth factor receptor; growth factor receptor; growth hormone; heregulin alpha1; heregulin beta1; methylxanthine derivative; neu differentiation factor; thyroid hormone; unclassified drug; adipocyte; animal cell; article; cell differentiation; cell migration; cell proliferation; controlled study; epithelium cell; malignant transformation; mitogenesis; nonhuman; oncogene neu; polyacrylamide gel electrophoresis; priority journal; proadipocyte; protein expression; protein phosphorylation; 3T3-L1 Cells; Adipocytes; Animals; Cell Differentiation; Dexamethasone; Epidermal Growth Factor; Humans; Insulin; Mice; Neuregulin-1; Phosphorylation; Receptor, Epidermal Growth Factor; Receptor, erbB-2; Tumor Cells, Cultured; Murinae
Año:2003
Volumen:90
Número:3
Página de inicio:561
Página de fin:572
DOI: http://dx.doi.org/10.1002/jcb.10647
Título revista:Journal of Cellular Biochemistry
Título revista abreviado:J. Cell. Biochem.
ISSN:07302312
CODEN:JCEBD
CAS:dexamethasone, 50-02-2; growth hormone, 36992-73-1, 37267-05-3, 66419-50-9, 9002-72-6; Dexamethasone, 50-02-2; Epidermal Growth Factor, 62229-50-9; heregulin alpha; heregulin beta1, 155646-83-6; Insulin, 11061-68-0; Neuregulin-1; Receptor, Epidermal Growth Factor, EC 2.7.1.112; Receptor, erbB-2, EC 2.7.1.112
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07302312_v90_n3_p561_Pagano

Referencias:

  • Adachi, H., Kurachi, H., Homma, H., Adachi, K., Takashi, I., Morishige, K., Matsuzawa, Y., Miyake, A., Epidermal growth factor promotes adipogenesis of 3T3-L1 cell in vitro (1994) Endocrinology, 135, pp. 1824-1830
  • Ailhaud, G., Obesity and the adipocyte. Molecular mechanisms of adipocyte differentiation (1997) J Endocr, 155, pp. 201-202
  • Balaña, M.E., Labriola, L., Salatino, M., Movsichoff, F., Peters, G., Charreau, E.H., Elizalde, P., Activation of erbB-2 via a hierarchical interaction between erbB-2 and type I insulin-like growth factor receptor in mammary tumor cells (2001) Oncogene, 20, pp. 34-47
  • Barbier, A., Poppleton, H., Yigzaw, Y., Mullenix, J., Wiepz, G., Bertics, P., Patel, T., Transmodulation of epidermal growth factor receptor function by cyclic AMP-dependent protein kinase (1999) J Biol Chem, 274, pp. 14067-14073
  • Bates, N.P., Hurst, H.C., Transcriptional regulation of type I receptor tyrosine kinases in the mammary gland (1997) J Mammary Gland Biol Neoplasia, 2, pp. 153-163
  • Boney, C.M., Smith, R.M., Gruppuso, P.A., Modulation of insulin-like growth factor 1 mitogenic signaling in 3T3-L1 preadipocyte differentiation (1998) Endocrinology, 139, pp. 1638-1644
  • Carraway K.L. III, Burden, S.J., Neuregulins and their receptors (1995) Curr Opin Neurobiol, 5, pp. 606-612
  • Coppola, D., Ferber, A., Miura, M., Sell, C., D'Ambrosio, C., Rubin, R., Baserga, R., A functional insulin-like growth factor 1 receptor is required for the mitogenic and transforming activities of the epidermal growth factor receptor (1994) Mol Cell Biol, 14 (7), pp. 4588-4595
  • Croxtall, J.D., Choudhury, Q., Flower, R.J., Glucocorticoids act within minutes to inhibit recruitment of signaling factors to activated EGF receptors through a receptor-dependent, transcription-independent mechanism (2000) Br J Pharmacol, 130, pp. 289-298
  • Darcy, K.M., Zangani, D., Wohlhueter, A.L., Huang, R.Y., Vaughan, M.M., Russell, J.A., Ip, M.M., Changes in erbB2 [her-2/neu], erbB3, and erbB4 during growth, differentiation, and apoptosis of normal rat mammary epithelial cells (2000) J Histochem Cytochem, 48, pp. 63-80
  • De Bortoli, M., Dati, C., Hormonal regulation of type I receptor tyrosine kinases expression in the mammary gland (1997) J Mammary Gland Biol Neoplasia, 2, pp. 175-185
  • Graus-Porta, D., Beerli, R.R., Daly, J.M., Hynes, N.E., ErbB-2, the preferred heterodimerization partner of all erbB receptors, is a mediator of lateral signaling (1997) EMBO J, 16, pp. 1647-1655
  • Green, H., Kehinde, O., Sublines of mouse 3T3 cells that accumulate lipid (1974) Cell, 1, pp. 113-116
  • Hardy, R.W., Gupta, K.B., McDonals, J.M., Williford, J., Wells, A., Epidermal growth factor (EGF) receptor carboxiterminal domains are required for EGF-induced glucose transport in transgenic 3T3-L1 adipocytes (1995) Endocrinology, 136, pp. 431-439
  • Hynes, N.E., Stern, D.F., The biology of erbB-2/HER-2 and its role in cancer (1994) Biochim Biophys Acta, 1198, pp. 165-184
  • Junttila, T.T., Sundvall, M., Maatta, J.A., Elenius, K., ErbB4 and its isoforms: Selective regulation of growth factor responses by naturally occurring receptor variants (2000) Trends Cardiovasc Med, 10, pp. 304-310
  • Karlan, B., Jones, J., Slamon, D., Lagasse, L., Glucocorticoids stabilize Her-2/neu messenger RNA in human epithelial ovarian carcinoma cells (1994) Gynecol Oncol, 53, pp. 421-426
  • Kim, S., Houtman, J.C.D., Jiang, J., Ruppert, M., Bertics, P.J., Frank, S.J., Growth hormone-induced alteration in erbB-2 phosphorylation status in 3T3-F442A fibroblasts (1999) J Biol Chem, 274, pp. 36015-36024
  • Lee, H., Maihle, N., Isolation and characterization of four alternate c-erbB3 transcripts expressed in ovarian carcinoma-derived cell lines and normal human tissues (1998) Oncogene, 16, pp. 3243-3252
  • Lim, H., Dey, S.K., Das, S.K., Differential expression of the erbB2 gene in the periimplantation mouse uterus: Potential mediator of signaling by epidermal growth factor-like growth factors (1997) Endocrinology, 138, pp. 1328-1337
  • Markogiannakis, E., Georgoulias, V., Margioris, A., Zoumakis, E., Stournaras, C., Gravanis, A., Estrogens and glucocorticoids induce the expression of c-erbB2/NEU receptor in Ishikawa human endometrial cells (1997) Life Sci, 61, pp. 1083-1095
  • Morrison, R.F., Farmer, S.R., Symposium: Adipocyte function, differentiation and metabolism. Hormonal signaling and transcriptional control of adipocyte differentiation (2000) J Nutr, 130, pp. 3116S-3121S
  • Mulligan, C., Rochford, J., Denyer, G., Stephens, R., Yeo, G., Freeman, T., Siddle, K., O'Rahilly, S., Microarray analysis of insulin and insulin-growth factor-1 (IGF-1) receptor signaling reveals the selective up-regulation of the mitogen heparin-binding EGF-like growth factor by IGF-1 (2002) J Biol Chem, 277 (45), pp. 42480-42487
  • Olayioye, M.A., Neve, R., Lane, H.A., Hynes, N.E., The ErbB signaling network: Receptor heterodimerization in development and cancer (2000) EMBO J, 19, pp. 3159-3167
  • Peles, E., Bacus, S.S., Koski, R.A., Lu, H.S., Wen, D., Ogden, S.G., Levy, R.B., Yarden, Y., Isolation of the neu/HER-2 stimulatory ligand: A 44 kDa glycoprotein that induces differentiation of mammary tumor cells (1992) Cell, 69, pp. 205-216
  • Press, M.F., Cordon-Cardo, C., Slamon, D.J., Expression of the HER-2/neu proto-oncogene in normal human adult and fetal tissues (1990) Oncogene, 5, pp. 953-962
  • Reed, B.C., Kaufmann, S.H., Mackall, J.C., Student, A.K., Lane, M.D., Alterations in insulin binding accompaying differentiation of 3T3-L1 preadipocytes (1977) Proc Natl Acad Sci USA, 74, pp. 4876-4880
  • Roudabush, F.L., Pierce, K.L., Maudsley, S., Khan, K.D., Lutrell, L.M., Tansactivation of the EGF receptor mediates IGF-1-stimulated Shc phosphorylation and ERK1/2 activation in COS-7 cells (2000) J Biol Chem, 275, pp. 22583-22589
  • Schmidt, W., Pöll-Jordan, G., Löffler, G., Adipose convertion of 3T3-L1 cells in a serum-free culture system depends on epidermal growth factor, insulin-like growth factor-1, corticosterone, and cyclic AMP (1990) J Biol Chem, 265, pp. 15489-15495
  • Schroeder, J.A., Lee, D.C., Dynamic expression and activation of ERBB receptors in the developing mouse mammary gland (1998) Cell Growth Differ, 9, pp. 451-464
  • Serrero, G., EGF inhibits the differentiation of adipocyte precursors in primary cultures (1987) Biochem Biophys Res Commun, 146, pp. 194-202
  • Serrero, G., Mills, D., Physiologic role of EGF on adipose tissue development in vivo (1991) Proc Natl Acad Sci USA, 88, pp. 3912-3916
  • Sliwkowski, M.X., Schaefer, G., Akita, R.W., Lofgren, J.A., Fitzpatrick, V.D., Nuijens, A., Fendly, B.M., Carraway K.L. III, Coexpression of erbB2 and erbB3 proteins reconstitutes a high affinity receptor for heregulin (1994) J Biol Chem, 269, pp. 14661-14665
  • Smith, P.J., Wise, L.S., Berkowitz, R., Wan, C., Rubin, C.S., Insulin-like growth factor-1 is an essential regulator of the differentiation of 3T3-L1 adipocytes (1988) J Biol Chem, 263, pp. 9402-9408
  • Srivinisan, R., Leverton, K., Sheldon, H., Hurst, H., Sarraf, C., Gullick, W., Intracellular expression of the truncated extracellular domain of c-erbB3/HER3 (2001) Cell Signal, 13, pp. 321-330
  • Stern, D.F., Kamps, M.P., EGF-stimulated tyrosine phosphorylation of p185neu: A potential model for receptor interactions (1988) EMBO J, 7, pp. 995-1001
  • Yao, H., Labudda, K., Rim, C., Loda, M., Stork, P.J., Cyclic adenosine monophosphate can convert epidermal growth factor into a differentiating factor in neuronal cells (1995) J Biol Chem, 270, pp. 20748-20753
  • Yao, H., York, R.D., Misra-Press, A., Carr, D.W., Stork, P.J., The cyclic adenosine monophosphate-dependent protein kinase (PKA) is required for the sustained activation of mitogen-activated kinases and gene expression by nerve growth factor (1998) J Biol Chem, 273, pp. 8240-8247
  • Zizola, C., Balaña, M.E., Sandoval, M., Calvo, J.C., Changes in IGF-1 receptor and IGF-1 mRNA during differentiation of 3T3-L1 preadipocytes (2002) Biochimie, 84, pp. 975-980

Citas:

---------- APA ----------
Pagano, E. & Calvo, J.C. (2003) . ErbB2 and EGFR are downmodulated during the differentiation of 3T3-L1 preadipocytes. Journal of Cellular Biochemistry, 90(3), 561-572.
http://dx.doi.org/10.1002/jcb.10647
---------- CHICAGO ----------
Pagano, E., Calvo, J.C. "ErbB2 and EGFR are downmodulated during the differentiation of 3T3-L1 preadipocytes" . Journal of Cellular Biochemistry 90, no. 3 (2003) : 561-572.
http://dx.doi.org/10.1002/jcb.10647
---------- MLA ----------
Pagano, E., Calvo, J.C. "ErbB2 and EGFR are downmodulated during the differentiation of 3T3-L1 preadipocytes" . Journal of Cellular Biochemistry, vol. 90, no. 3, 2003, pp. 561-572.
http://dx.doi.org/10.1002/jcb.10647
---------- VANCOUVER ----------
Pagano, E., Calvo, J.C. ErbB2 and EGFR are downmodulated during the differentiation of 3T3-L1 preadipocytes. J. Cell. Biochem. 2003;90(3):561-572.
http://dx.doi.org/10.1002/jcb.10647