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Abstract:

There is evidence involving protein kinase C (PKC) in the signal transduction pathways that regulate the differentiation of myoblasts into mature multinucleated muscle cells (myotubes). In order to obtain information on the possible role of individual PKC isozymes in myogenesis, in the present work we investigated the differential expression of PKC isoforms α, β, δ, ε, and ζ during muscle cell development in vitro. Chick embryo myoblasts cultured from 1 to 6 days were used as experimental model. Morphological characterization and measurement of specific biochemical parameters in cultures, e.g., DNA synthesis, creatine kinase activity, and myosin levels, revealed a typical muscle cell developmental pattern consisting of an initial proliferation of myoblasts followed by their differentiation into myotubes. PKC activity was high at the proliferative stage, decreased as myoblasts elongated and fused, and increased again in differentiated myotubes. In proliferating myoblasts, the PKC inhibitors calphostin C and bisindolylmaleimide I decreased DNA synthesis whereas in myoblasts undergoing differentiation they exerted the opposite effect, suggesting that PKC plays a role at both stages of myogenesis. Western blot analysis of changes in the expression of PKC isoforms during muscle cell development showed high levels of PKC α in the proliferating phase which markedly decreased as myoblasts differentiated. Treatment with TPA of proliferative myoblasts inhibited DNA synthesis and selectively down-regulated PKC α, suggesting that this isozyme may have an important role in maintaining myoblast proliferation. On the other hand, an increase in the expression of PKC β, δ, and ε was detected during myogenesis, suggesting that one or more of these isoforms may participate in the differentiation process of myoblasts.

Registro:

Documento: Artículo
Título:Evidence on the participation of protein kinase C α in the proliferation of cultured myoblasts
Autor:Capiati, D.A.; Limbozzi, F.; Téllez-Iñón, M.T.; Boland, R.L.
Filiación:Depto. Biologia, Bioquimica Farmacia, Universidad Nacional del Sur, 8000 Bahía Blanca, Argentina
Inst. Invest. Ing. Genetica B., CONICET, 1428 Buenos Aires, Argentina
Depto. Biologia, Bioquimica Farmacia, Universidad Nacional del Sur, S. Juan 670-8000 Bahía Blanca, Argentina
Palabras clave:DNA synthesis; Myoblasts; Myogenesis; PKC isozymes; Protein kinase C; creatine kinase; DNA; isoenzyme; myosin; protein kinase c; animal cell; article; cell culture; cell differentiation; cell proliferation; chick embryo; controlled study; DNA synthesis; embryo; enzyme activity; muscle development; myoblast; nonhuman; priority journal; protein expression; Animals; Cell Differentiation; Cell Division; Chick Embryo; DNA Replication; Indoles; Isoenzymes; Maleimides; Muscle, Skeletal; Naphthalenes; Protein Kinase C; Protein Kinase C-alpha; Tetradecanoylphorbol Acetate; Animalia
Año:1999
Volumen:74
Número:2
Página de inicio:292
Página de fin:300
DOI: http://dx.doi.org/10.1002/(SICI)1097-4644(19990801)74:2<292::AID-JCB13>3.0.CO;2-M
Título revista:Journal of Cellular Biochemistry
Título revista abreviado:J. Cell. Biochem.
ISSN:07302312
CODEN:JCEBD
CAS:bisindolylmaleimide I, 133052-90-1; calphostin C, 121263-19-2; Indoles; Isoenzymes; Maleimides; Naphthalenes; Protein Kinase C, EC 2.7.1.37; Protein Kinase C-alpha, EC 2.7.1.37; Tetradecanoylphorbol Acetate, 16561-29-8
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07302312_v74_n2_p292_Capiati

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Citas:

---------- APA ----------
Capiati, D.A., Limbozzi, F., Téllez-Iñón, M.T. & Boland, R.L. (1999) . Evidence on the participation of protein kinase C α in the proliferation of cultured myoblasts. Journal of Cellular Biochemistry, 74(2), 292-300.
http://dx.doi.org/10.1002/(SICI)1097-4644(19990801)74:2<292::AID-JCB13>3.0.CO;2-M
---------- CHICAGO ----------
Capiati, D.A., Limbozzi, F., Téllez-Iñón, M.T., Boland, R.L. "Evidence on the participation of protein kinase C α in the proliferation of cultured myoblasts" . Journal of Cellular Biochemistry 74, no. 2 (1999) : 292-300.
http://dx.doi.org/10.1002/(SICI)1097-4644(19990801)74:2<292::AID-JCB13>3.0.CO;2-M
---------- MLA ----------
Capiati, D.A., Limbozzi, F., Téllez-Iñón, M.T., Boland, R.L. "Evidence on the participation of protein kinase C α in the proliferation of cultured myoblasts" . Journal of Cellular Biochemistry, vol. 74, no. 2, 1999, pp. 292-300.
http://dx.doi.org/10.1002/(SICI)1097-4644(19990801)74:2<292::AID-JCB13>3.0.CO;2-M
---------- VANCOUVER ----------
Capiati, D.A., Limbozzi, F., Téllez-Iñón, M.T., Boland, R.L. Evidence on the participation of protein kinase C α in the proliferation of cultured myoblasts. J. Cell. Biochem. 1999;74(2):292-300.
http://dx.doi.org/10.1002/(SICI)1097-4644(19990801)74:2<292::AID-JCB13>3.0.CO;2-M