Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Homeobox-containing genes encode transcriptional regulators involved in cell fate and pattern formation during embryogenesis. Recently, it has become clear that their expression in continuously developing adult tissues, as well as in tumorigenesis, may be of equal importance. In the mouse mammary gland, expression patterns of several homeobox genes suggest a role in epithelial- stromal interactions. Because the stroma and the extracellular matrix (ECM) are known to influence both functional and morphological development of the mammary gland, we asked whether these genes would be expressed postnatally in the gland and also in cell lines in culture and whether they could be modulated by ECM. Using a polymerase chain reaction-base strategy five members of the Hox gene clusters a and b were shown to be expressed in cultured mouse mammary cells. Hoxa-1 and Hoxb-7 were chosen for further analysis. Hoxb-7 was chosen because it had not been described previously in the mammary gland and was modulated at different stages of gland development. Hoxa-1 was chosen because it was reported previously to be expressed only in mammary tumors, and not in normal glands. We showed that culturing the mammary epithelial cell lines SCp2 and CID-9 on a basement membrane (BM) that was previously shown to induce a lactational phenotype was necessary to turn off Hoxb-7, but a change in cell shape, brought about by culturing the cells on an inert substratum such as polyHEMA, was sufficient to downregulate Hoxa- 1. This is the first report of modulation of homeobox genes by ECM. The results provide a rationale for the differential pattern of expression in vivo of Hoxa-1 and Hoxb-7 during different stages of development. The culture model should permit further in-depth analysis of the molecular mechanisms involved in how ECM signaling and homeobox genes may interact to bring about tissue organization.

Registro:

Documento: Artículo
Título:Expression of Hoxa-1 and Hoxb-7 is regulated by extracellular matrix- dependent signals in mammary epithelial cells
Autor:Srebrow, A.; Friedmann, Y.; Ravanpay, A.; Daniel, C.W.; Bissell, M.J.
Filiación:Lawrence Berkeley Natl. Laboratory, Berkeley, CA 94720, United States
Department of Biology, University of California, Santa Cruz, CA 95064, United States
Life Sciences Division, Lawrence Berkeley Natl. Laboratory, MS 83-101, One Cyclotron Rd., Berkeley, CA 94720, United States
Lab. de Fisiol. y Biologia Molecular, Departamento de Ciencias Biologicas, Pabellon II, (1428) Buenos Aires, Argentina
Palabras clave:Basement membrane; Gene expression; Homeobox; Mammary gland; Morphogenesis; homeodomain protein; animal cell; animal tissue; article; basement membrane; breast development; breast epithelium; cell shape; controlled study; embryo; embryo development; extracellular matrix; gene cluster; gene expression regulation; homeobox; mouse; nonhuman; polymerase chain reaction; priority journal; signal transduction; transcription regulation; Adenocarcinoma; Animals; Base Sequence; Basement Membrane; Cell Cycle; Cell Differentiation; Cell Size; Cells, Cultured; Cloning, Molecular; Epithelial Cells; Extracellular Matrix; Female; Gene Expression Regulation, Developmental; Genes, Homeobox; Homeodomain Proteins; Lactation; Mammary Glands, Animal; Mammary Neoplasms, Animal; Mice; Molecular Sequence Data; Pregnancy; RNA, Messenger; Signal Transduction; Transcription Factors; Animalia
Año:1998
Volumen:69
Número:4
Página de inicio:377
Página de fin:391
DOI: http://dx.doi.org/10.1002/(SICI)1097-4644(19980615)69:4<377::AID-JCB1>3.0.CO;2-K
Título revista:Journal of Cellular Biochemistry
Título revista abreviado:J. Cell. Biochem.
ISSN:07302312
CODEN:JCEBD
CAS:homeobox A1 protein; Homeodomain Proteins; Hoxb7 protein, mouse; RNA, Messenger; Transcription Factors
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07302312_v69_n4_p377_Srebrow

Referencias:

  • Aberdam, D., Negreanu, V., Sachs, L., Blatt, C., The oncogenic potential of an activated Hox-2.4 homeobox gene in mouse fibroblasts (1991) Mol Cell Biol, 11, pp. 554-557
  • Adams, J.C., Watt, F.M., Regulation of development and differentiation by the extracellular matrix (1993) Development, 117, pp. 1183-1198
  • Aggeler, J., Ward, J., Blackie, L.M., Barcellos-Hoff, M.H., Streuli, C.H., Bissell, M.J., Cytodifferentiation of mouse mammary epithelial cells cultured on a reconstituted basement membrane reveals striking similarities to development in vivo (1991) J Cell Sci, 99, pp. 407-417
  • Ashkenas, J., Muschler, J., Bissell, M.J., The extracellular matrix in epithelial biology: Shared molecules and common themes in distant phyla (1996) Dev Biol, 180, pp. 433-444
  • Barcellos-Hoff, M.H., Aggeler, J., Ram, T.G., Bissell, M.J., Functional differentiation and alveolar morphogenesis of primary mammary cultures on reconstituted basement membrane (1989) Development, 105, pp. 223-235
  • Bissell, M.J., Hall, H.G., Form and function in the mammary gland - The role of extracellular matrix (1987) The Mammary Gland, pp. 97-146. , Neville, MC, Daniel CW (eds): New York: Plenum Press
  • Blaschke, R., Andres, A.C., Reid, H.H., Zurcher, G., Friis, R.R., Ziemiecki, A., Tyrosine kinases: From viral oncogenes to developmental regulators (1991) Behring Inst Mitt, 89, pp. 81-92
  • Boudreau, N., Sympson, C.J., Werb, Z., Bissell, M.J., Suppression of ICE and apoptosis in mammary epithelial cells by extracellular matrix (1995) Science, 267, pp. 891-893
  • Boudreau, N., Werb, Z., Bissell, M.J., Suppression of apoptosis by basement membrane requires three-dimensional tissue organization and withdrawal from the cell cycle (1996) Proc Natl Acad Sci USA, 93, pp. 3509-3513
  • Chen, L.-H., Bissell, M.J., A novel regulatory mechanism for whey acidic protein gene expression (1989) Cell Regul, 1, pp. 45-54
  • Cillo, C., Barba, P., Freschi, G., Bucciarelli, G., Magli, M.C., Boncinelli, E., HOX gene expression in normal and neoplastic human kidney (1992) Int J Cancer, 51, pp. 892-897
  • Cunha, G.R., Young, P., Hamamoto, S., Guzman, R., Nandi, S., Developmental Responses of adult mammary epithelial cells to various fetal and neonatal mesenchymes (1992) Epithelial Cell Biol, 1, pp. 105-118
  • Chomczynski, P., Sacchi, N., Single step method of RNA isolation by acid guanidinium thiocyanate-phenolchloroform extraction (1987) Anal Biochem, 162, pp. 156-159
  • Danielson, K.G., Oborn, C.J., Durban, E.M., Butel, J.S., Medina, D., Epithelial mouse mammary cell line exhibiting normal morphological genesis in vivo and functional differentiation in vitro (1984) Proc Natl Acad Sci USA, 81, pp. 3756-3760
  • Desprez, P.Y., Roskelley, C., Campisi, J., Bissell, M.J., Isolation of functional cell lines from a mouse mammary epithelial cell strain: The importance of basement membrane and cell-cell interaction (1993) Mol Cell Diff, 1, pp. 99-110
  • Desprez, P.Y., Hara, E., Bissell, M.J., Campisi, J., Suppression of mammary epithelial cell differentiation by the helix-loop-helix protein Id-1 (1995) Mol Cell Biol, 15, pp. 3398-3404
  • De Vita, G., Barba, P., Odartchenko, N., Givel, J.C., Freschi, G., Bucciarelli, G., Magli, M.C., Cillo, C., Expression of homeobox-containing genes in primary and metastatic colorectal cancer (1993) Eur J Cancer, 29, pp. 887-893
  • Emerman, J.T., Vogl, A.W., Cell size and shape changes in the myoepithelium of the mammary gland during differentiation (1986) Anat Rec, 216, pp. 405-415
  • Folkman, J., Moscona, A., Role of cell shape in growth control (1978) Nature, 273, pp. 345-349
  • Friedmann, Y., Daniel, C.W., Regulated expression of homeobox genes Msx-1 and Msx-2 in mouse mammary gland development suggests a role in hormone action and epithelial-stromal interactions (1996) Dev Biol, 177, pp. 347-355
  • Friedmann, Y., Daniel, C.A., Strickland, P., Daniel, C.W., Hox genes in normal and neoplastic mouse mammary gland (1994) Cancer Res, 54, pp. 5981-5985
  • Frohman, M.A., Boyle, M., Martin, G.R., Isolation of the mouse Hox-2.9 gene: Analysis of embryonic expression suggests that positional information along the anterior-posterior axis is specified by mesoderm (1990) Development, 110, pp. 589-607
  • Gehring, W.J., Qian, Y.Q., Billeter, M., Furukubo-Tokunaga, K., Schier, A.F., Rosendez-Perez, D., Affolter, M., Wüthrich, K., Homeodomain-DNA recognition (1994) Cell, 78, pp. 211-223
  • Giampaolo, A., Sterpetti, P., Bulgarini, D., Samoggia, P., Pelosi, E., Valtieri, M., Peschle, C., Key functional role and lineage-specific expression of selected HOXB genes in purified hematopoietic progenitor differentiation (1994) Blood, 84, pp. 3637-3647
  • Goomer, R.S., Holst, B.D., Wood, I.C., Jones, F.S., Edelman, G.M., Regulation in vitro of an L-CAM enhancer by homeobox genes HoxD9 and HNF-1 (1994) Proc Natl Acad Sci USA, 91, pp. 7985-7989
  • He, X., Treacy, M.N., Simmons, D.M., Ingraham, H.A., Swanson, L.W., Rosenfeld, M.G., Expression of a large family of POU-domain regulatory genes in mammalian brain development (1989) Nature, 340, pp. 35-41
  • Howlett, A.R., Bailey, N., Damsky, C., Petersen, O.W., Bissell, M.J., Cellular growth and survival are mediated by β1 integrins in normal human breast epithelium but not in breast carcinoma (1995) J Cell Sci, 108, pp. 1945-1957
  • James, R., Kazenwadel, J., Homeobox gene expression in the intestinal epithelium of adult mice (1991) J Biol Chem, 266, pp. 3246-3251
  • Jones, F., Chalepakis, G., Gruss, P., Edelman, G.M., Activation of the cytotactin promoter by the homeobox-containing gene Evx-1 (1992) Proc Natl Acad Sci USA, 89, pp. 2091-2095
  • Jones, F.S., Prediger, E.A., Bittner, D.A., De Robertis, E.M., Edelman, G.M., Cell adhesion molecules as targets for Hox genes: Neural cell adhesion molecule promoter activity is modulated by cotransfection with Hox-2.5 and -2.4 (1992) Proc Natl Acad Sci USA, 89, pp. 2086-2090
  • Jones, F.S., Holst, B.D., Minowa, O., De Robertis, E.M., Edelman, G.M., Binding and transcriptional activation of the promoter for the neural cell adhesion molecule by HoxC6 (Hox-3.3) (1993) Proc Natl Acad Sci USA, 90, pp. 6557-6561
  • Jones, J.C., Kurpakus, M.A., Cooper, H.M., Quaranta, V., A funtion for the integrin α6β4 in the hemidesmosome (1991) Cell Regul, 2, pp. 427-438
  • Jones, J.L., Royall, J.E., Critchley, D.R., Walker, R.A., Modulation of myoepithelial-associated α6β4 integrin in a breast cancer cell line alters invasive potential (1997) Exp Cell Res, 235, pp. 325-333
  • Jones, P.L., Boudreau, N., Myers, C.A., Erickson, H.P., Chiquet-Ehrismann, R., Bissell, M.J., Tenascin-C inhibits extracellular matrix-dependent gene expression in mammary epithelial cells - Localization of active regions using recombinant tenascin fragments (1995) J Cell Sci, 100, pp. 519-527
  • Jowett, A.K., Vainio, S., Ferguson, M.W.J., Sharpe, P.T., Thelsteff, I., Epithelial-mesenchymal interactions are requires for msx-1 and msx-2 gene expression in the developing murine molar tooth (1993) Development, 117, pp. 461-470
  • Kenyon, C., If birds can fly, why can't we? Homeotic genes and evolution (1994) Cell, 78, pp. 175-180
  • Kleinman, H.K., McGarvey, M.L., Hassell, J.R., Star, V.L., Cannon, F.B., Laurie, G.W., Martin, G.R., Basement membrane complexes with biological activity (1986) Biochemistry, 25, pp. 312-318
  • Krumlauf, R., Hox genes in vertebrate development (1994) Cell, 78, pp. 191-201
  • Lai, E., Prezioso, V.R., Tao, W., Chen, W.S., Darnell Jr., J.E., Hepatocyte nuclear factor 3a belongs to a gene family in mammals that is homologous to the Drosophila homeotic gene fork head (1991) Genes Dev, 5, pp. 416-427
  • Lawrence, H.J., Johnson, R.A., Perrine, S., Largman, C., The role of homeobox genes in erythropoiesis (1994) Ann NY Acad Sci, 718, pp. 165-180
  • Lawrence, P.A., Morata, G., Homeobox genes: Their function in Drosophila segmentation and pattern formation (1994) Cell, 78, pp. 181-189
  • Levine, E.M., Schechter, N., Homeobox genes are expressed in the retina and brain of adult goldfish (1993) Proc Natl Acad Sci USA, 90, pp. 2729-2733
  • Li, M.L., Aggeler, J., Farson, D.A., Hatier, C., Hassell, J., Bissell, M.J., Influence of a reconstituted basement membrane and its components on casein gene expression and secretion in mouse mammary epithelial cells (1987) Proc Natl Acad Sci USA, 84, pp. 136-140
  • Lill, M.C., Fuller, J.F., Herzig, R., Crooks, G.M., Gasson, J.C., The role of the homeobox gene, HOX B7, in human myelomonocytic differentiation (1995) Blood, 85, pp. 692-697
  • Lin, C.L.-Q., Bissell, M.J., Multi-faceted regulation of cell differentiation by extracellular matrix (1993) FASEB J, 7, pp. 737-743
  • Lin, C., Dempsey, P.J., Coffey, R.J., Bissell, M.J., Extracellular matrix regulates whey acidic protein gene expression by suppression of TGF-α in mouse mammary epithelial cells - Studies in culture and in transgenic mice (1995) J Cell Biol, 129, pp. 1115-1126
  • Lochter, A., Srebrow, A., Sympson, C.J., Terracio, N., Werb, Z., Bissell, M.J., Misregulation of stromelysin-1 expression in mouse mammary tumor cells accompanies acquisition of stromelysin-1-dependent invasive properties (1997) J Biol Chem, 272, pp. 5007-5015
  • Lopez, A.J., Developmental role of transcription factor isoforms generated by alternative splicing (1995) Dev Biol, 172, pp. 396-411
  • Maniatis, T., Fritsch, E.F., Sambrook, J., (1989) Molecular Cloning: A Laboratory Manual, , Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
  • Martins-Green, M., Bissell, M.J., Cell-extracellular matrix interactions in development (1995) Semin Dev Biol, 6, pp. 149-159
  • Maulbecker, C.C., Gruss, P., The oncogenic potential of deregulated homeobox genes (1993) Cell Growth Diff, 4, pp. 431-441
  • McGinnis, W., Krumlauf, R., Homeobox genes and axial patterning (1992) Cell, 68, pp. 283-302
  • Meijlink, F., De Laaf, R., Verrijzer, P., Destree, O., Kroezen, V., Hilkens, J., Deschamps, J., A mouse homeobox containing gene on chromosome 11: Sequence and tissue-specific expression (1987) Nucleic Acids Res, 15, pp. 6773-6786
  • Pavlova, A., Boutin, E., Cunha, G., Sassoon, D., Msx-1 (Hox-7.1) in the adult mouse uterus: Cellular interactions underlying regulation of expression (1994) Development, 120, pp. 335-346
  • Petersen, O.W., Ronnov-Jessen, L., Howlett, A.R., Bissell, M.J., Interaction with basement membrane serves to rapidly distinguish growth and differentiation pattern of normal and malignant human breast epithelial cells (1992) Proc Natl Acad Sci USA, 89, pp. 9064-9068
  • Roskelley, C.D., Deprez, P.Y., Bissell, M.J., Extracellular matrix-dependent tissue-specific gene expression in mammary epithelial cells requires both physical and biochemical signal transduction (1994) Proc Natl Acad Sci USA, 91, pp. 12378-12382
  • Roskelley, C., Srebrow, A., Bissell, M.J., A hierarchy of extracellular matrix-mediated signalling regulates tissue-specific gene expression (1995) Curr Opin Cell Biol, 7, pp. 736-747
  • Rudnick, A., Ling, T.Y., Odagiri, H., Rutter, W.J., German, M.S., Pancreatic beta cells express a diverse set of homeobox genes (1994) Proc Natl Acad Sci USA, 91, pp. 12203-12207
  • Sakakura, T., Sakagami, Y., Nishizuka, Y., Persistence of responsiveness of adult mouse mammary gland to induction by embryonic mesenchyme (1979) Dev Biol, 72, pp. 201-210
  • Scott, M., Vertebrate homeobox gene nomenclature (1992) Cell, 71, pp. 551-553
  • Schmidhauser, C., Bissell, M.J., Myers, C.A., Casperson, G.F., Extracellular matrix and hormones transcriptionally regulate bovine β-casein 5′ sequences in stably transfected mouse mammary cells (1990) Proc Natl Acad Sci USA, 87, pp. 9118-9122
  • Schmidhauser, C., Casperson, G.F., Myers, C.A., Sanzo, K.T., Bolten, S., Bissell, M.J., A novel transcriptional enhancer is involved in the prolactin and extracellular matrix-dependent regulation of β-casein gene expression (1992) Mol Biol Cell, 3, pp. 699-709
  • Silberstein, G.B., Flanders, K.C., Roberts, A.B., Daniel, C.W., Regulation of mammary morphogenesis: Evidence for extracellular matrix-mediated inhibition of ductal budding by transforming growth factor-β1 (1992) Dev Biol, 152, pp. 354-362
  • Song, K., Wang, Y., Sassoon, D., Expression of Hox-7.1 in myoblasts inhibits terminal differentiation and induces cell transformation (1992) Nature, 360, pp. 477-481
  • Strange, R., Li, F., Saurer, S., Burkhardt, A., Friis, R.R., Apoptotic cell death and tissue remodeling during mouse mammary gland involution (1992) Development, 115, pp. 49-58
  • Streuli, C.H., Bailey, N., Bissell, M.J., Control of mammary epithelial differentiation: Basement membrane induces tissue-specific gene expression in the absence of cell-cell interaction and morphological polarity (1991) J Cell Biol, 115, pp. 1383-1395
  • Streuli, C.H., Schmidhauser, C., Kobrin, M., Bissell, M.J., Derynck, R., Extracellular matrix regulates expression of the TGF-β1 gene (1993) J Cell Biol, 120, pp. 253-260
  • Streuli, C.H., Schmidhauser, C., Bailey, N., Yurchenco, P., Skubitz, A.P.N., Roskelley, C., Bissell, M.J., A domain within laminin that mediates tissue-specific gene expression in mammary epithelia (1995) J Cell Biol, 129, pp. 591-603
  • Sympson, C.J., Talhouk, R.S., Alexander, C.M., Chin, S.K., Clift, S.M., Bissell, M.J., Werb, Z., Targeted expression of stromelysin-1 in mammary gland provides evidence for a role of proteinases in branching morphogenesis and the requirement for an intact basement membrane for tissue-specific gene expression (1994) J Cell Biol, 125, pp. 681-693
  • Sympson, C.J., Bissell, M.J., Werb, Z., Mammary gland tumor formation in transgenic mice overexpressing stromelysin-1 (1995) Semin Cancer Biol, 6, pp. 159-163
  • Takeshita, K., Bollekens, J.A., Hijiya, N., Ratajczak, M., Ruddle, F.H., Gewirtz, A.M., A homeobox gene of the Antennapedia class is required for human adult erythropoiesis (1993) Proc Natl Acad Sci USA, 90, pp. 3535-3538
  • Talhouk, R., Bissell, M.J., Werb, Z., Coordinated expression of extracellular matrix-degrading proteinases and their inhibitors regulates mammary epithelial function during involution (1992) J Cell Biol, 118, pp. 1271-1282
  • Taniguchi, Y., Komatsu, N., Moriuchi, T., Overexpression of the HOX4A (HOXD3) homeobox gene in human erythroleukemia HEL cells results in altered adhesive properties (1995) Blood, 85, pp. 2786-2794
  • Tiberio, C., Barba, P., Magli, M.C., Arvelo, F., Le Chevalier, T., Poupon, M.F., Cillo, C., Hox gene expression in human small-cell lung cancers xenografted into nude mice (1994) Int J Cancer, 58, pp. 608-615
  • Wolf, G., Kuncio, G.S., Sun, M.J., Neilson, E.G., Expression of Homeobox genes in a proximal tubular cell line derived from adult mice (1991) Kidney Int, 39, pp. 1027-1033
  • Wolgemuth, D., Viviano, C.M., Gizang-Ginsberg, E., Frohman, M.A., Joyner, A.L., Martin, G., Differential expression of the mouse homeobox-containing gene Hox-1.4 during male germ cell differentiation and embryonic development (1987) Proc Natl Acad Sci USA, 84, pp. 5813-5817
  • Wu, J., Zhu, J.Q., Zhu, D.X., Scharfman, A., Lamblin, G., Han, K.K., Selective inhibition of normal murine myelopoiesis "in vitro" by a Hox2.3 antisense oligodeoxynucleotide (1992) Cell Mol Biol, 38, pp. 367-376

Citas:

---------- APA ----------
Srebrow, A., Friedmann, Y., Ravanpay, A., Daniel, C.W. & Bissell, M.J. (1998) . Expression of Hoxa-1 and Hoxb-7 is regulated by extracellular matrix- dependent signals in mammary epithelial cells. Journal of Cellular Biochemistry, 69(4), 377-391.
http://dx.doi.org/10.1002/(SICI)1097-4644(19980615)69:4<377::AID-JCB1>3.0.CO;2-K
---------- CHICAGO ----------
Srebrow, A., Friedmann, Y., Ravanpay, A., Daniel, C.W., Bissell, M.J. "Expression of Hoxa-1 and Hoxb-7 is regulated by extracellular matrix- dependent signals in mammary epithelial cells" . Journal of Cellular Biochemistry 69, no. 4 (1998) : 377-391.
http://dx.doi.org/10.1002/(SICI)1097-4644(19980615)69:4<377::AID-JCB1>3.0.CO;2-K
---------- MLA ----------
Srebrow, A., Friedmann, Y., Ravanpay, A., Daniel, C.W., Bissell, M.J. "Expression of Hoxa-1 and Hoxb-7 is regulated by extracellular matrix- dependent signals in mammary epithelial cells" . Journal of Cellular Biochemistry, vol. 69, no. 4, 1998, pp. 377-391.
http://dx.doi.org/10.1002/(SICI)1097-4644(19980615)69:4<377::AID-JCB1>3.0.CO;2-K
---------- VANCOUVER ----------
Srebrow, A., Friedmann, Y., Ravanpay, A., Daniel, C.W., Bissell, M.J. Expression of Hoxa-1 and Hoxb-7 is regulated by extracellular matrix- dependent signals in mammary epithelial cells. J. Cell. Biochem. 1998;69(4):377-391.
http://dx.doi.org/10.1002/(SICI)1097-4644(19980615)69:4<377::AID-JCB1>3.0.CO;2-K