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Abstract:

The widespread use of aluminum (Al) provides easy exposure of humans to the metal and its accumulation remains a potential problem. In vivo and in vitro assays have associated Al overload with anemia. To better understand the mechanisms by which Al affects human erythrocytes, morphological and biochemical changes were analyzed after long-term treatment using an in vitro model. The appearance of erythrocytes with abnormal shapes suggested metal interaction with cell surface, supported by the fact that high amounts of Al attached to cell membrane. Long-term incubation of human erythrocytes with Al induced signs of premature erythrocyte death (eryptosis), such as phosphatidylserine externalization, increased intracellular calcium, and band 3 degradation. Signs of oxidative stress, such as significant increase in reactive oxygen species in parallel with decrease in the amount of reduced glutathione, were also observed. These oxidative effects were completely prevented by the antioxidant N-acetylcysteine. Interestingly, erythrocytes were also protected from the prooxidative action of Al by the presence of erythropoietin (EPO). In conclusion, results provide evidence that chronic Al exposure may lead to biochemical and morphological alterations similar to those shown in eryptosis induced by oxidant compounds in human erythrocytes. The antieryptotic effect of EPO may contribute to enhance the knowledge of its physiological role on erythroid cells. Irrespective of the antioxidant mechanism, this property of EPO, shown in this model of Al exposure, let us suggest potential benefits by EPO treatment of patients with anemia associated to altered redox environment. © 2011 Wiley Periodicals, Inc.

Registro:

Documento: Artículo
Título:Oxidative stress due to aluminum exposure induces eryptosis which is prevented by erythropoietin
Autor:Vota, D.M.; Crisp, R.L.; Nesse, A.B.; Vittori, D.C.
Filiación:Departamento de Química Biológica, Universidad de Buenos Aires, Ciudad Universitaria, (C1428EHA), Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Ciudad Autónoma de Buenos Aires, Argentina
División Hematología Clínica, Departamento de Medicina, Hospital Nacional Alejandro Posadas, Buenos Aires, Argentina
Palabras clave:aluminum; erythrocytes; erythropoietin; oxidative stress; acetylcysteine; aluminum; calcium; glutathione; phosphatidylserine; reactive oxygen metabolite; recombinant erythropoietin; adult; anemia; article; calcium cell level; cell death; cell membrane; cell protection; cell shape; cell structure; cell surface; controlled study; eryptosis; erythrocyte; erythroid cell; human; human cell; long term exposure; normal human; oxidation reduction reaction; oxidative stress; priority journal; protein degradation; Adult; Aluminum; Anemia; Anion Exchange Protein 1, Erythrocyte; Erythrocyte Aging; Erythrocyte Membrane; Erythrocytes; Erythropoietin; Humans; Microscopy, Electron, Scanning; Oxidative Stress; Recombinant Proteins
Año:2012
Volumen:113
Número:5
Página de inicio:1581
Página de fin:1589
DOI: http://dx.doi.org/10.1002/jcb.24026
Título revista:Journal of Cellular Biochemistry
Título revista abreviado:J. Cell. Biochem.
ISSN:07302312
CODEN:JCEBD
CAS:acetylcysteine, 616-91-1; aluminum, 7429-90-5; calcium, 14092-94-5, 7440-70-2; glutathione, 70-18-8; recombinant erythropoietin, 113427-24-0, 122312-54-3, 130455-76-4, 148363-16-0, 154725-65-2, 879555-13-2; Aluminum, 7429-90-5; Anion Exchange Protein 1, Erythrocyte; EPO protein, human; Erythropoietin, 11096-26-7; Recombinant Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07302312_v113_n5_p1581_Vota

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Citas:

---------- APA ----------
Vota, D.M., Crisp, R.L., Nesse, A.B. & Vittori, D.C. (2012) . Oxidative stress due to aluminum exposure induces eryptosis which is prevented by erythropoietin. Journal of Cellular Biochemistry, 113(5), 1581-1589.
http://dx.doi.org/10.1002/jcb.24026
---------- CHICAGO ----------
Vota, D.M., Crisp, R.L., Nesse, A.B., Vittori, D.C. "Oxidative stress due to aluminum exposure induces eryptosis which is prevented by erythropoietin" . Journal of Cellular Biochemistry 113, no. 5 (2012) : 1581-1589.
http://dx.doi.org/10.1002/jcb.24026
---------- MLA ----------
Vota, D.M., Crisp, R.L., Nesse, A.B., Vittori, D.C. "Oxidative stress due to aluminum exposure induces eryptosis which is prevented by erythropoietin" . Journal of Cellular Biochemistry, vol. 113, no. 5, 2012, pp. 1581-1589.
http://dx.doi.org/10.1002/jcb.24026
---------- VANCOUVER ----------
Vota, D.M., Crisp, R.L., Nesse, A.B., Vittori, D.C. Oxidative stress due to aluminum exposure induces eryptosis which is prevented by erythropoietin. J. Cell. Biochem. 2012;113(5):1581-1589.
http://dx.doi.org/10.1002/jcb.24026