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Abstract:

Purpose. The aim of the present work is to study the interaction of phosphate salts with trehalose and sucrose in freeze-dried matrices, particularly the effect of the salts on the glass transition temperature (T g) of the sugars. Methods. Freeze-dried trehalose and sucrose systems containing different amounts of sodium or potassium phosphate were analyzed by differential scanning calorimetry to determine the Tg and by Fourier-transform infrared spectroscopy (FTIR) analysis to evaluate the strength of the interaction between sugars and phosphate ions. Results. Sucrose-phosphate mixtures show an increase in Tg up to 40°C in a broad pH range (4-9) compared to that of pure sucrose. Sucrose-phosphate mixtures exhibit a higher Tg than pure sucrose while retaining higher water contents. Trehalose-phosphate mixtures (having a Tg of 135°C at a pH of 8.8) are a better option than pure trehalose for preservation of labile materials. The -OH stretching of the sugars in the presence of phosphates decreases with increase in pH, indicating an increase in the sugar-phosphate interaction. Conclusions. Sugar-phosphate mixtures exhibit several interesting features that make them useful for lyophilization of labile molecules; Tg values much higher than those observed for the pure sugars can be obtained upon the addition of phosphate. © 2004 Springer Science+Business Media, Inc.

Registro:

Documento: Artículo
Título:Effect of pH, counter ion, and phosphate concentration on the glass transition temperature of freeze-dried sugar-phosphate mixtures
Autor:Ohtake, S.; Schebor, C.; Palecek, S.P.; De Pablo, J.J.
Filiación:Chemical and Biological Engineering Department, University of Wisconsin, Madison, WI 53706, United States
Departamento de Industrias, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria, (1428) Cd. Auton. de Buenos Aires, Argentina
Palabras clave:Freeze-drying; Glass transition, sucrose; Phosphate; Trehalose; hydroxyl group; phosphate; potassium dihydrogen phosphate; sodium dihydrogen phosphate; sucrose; trehalose; water; acidity; alkalinity; analytic method; article; differential scanning calorimetry; freeze drying; glass transition temperature; infrared spectroscopy; molecular interaction; pH; priority journal; temperature dependence; chemistry; freeze drying; pH; temperature; thermodynamics; Calorimetry, Differential Scanning; Freeze Drying; Hydrogen-Ion Concentration; Phosphates; Spectroscopy, Fourier Transform Infrared; Sucrose; Temperature; Thermodynamics; Trehalose; Water
Año:2004
Volumen:21
Número:9
Página de inicio:1615
Página de fin:1621
DOI: http://dx.doi.org/10.1023/B:PHAM.0000041456.19377.87
Título revista:Pharmaceutical Research
Título revista abreviado:Pharm. Res.
ISSN:07248741
CODEN:PHREE
CAS:phosphate, 14066-19-4, 14265-44-2; potassium dihydrogen phosphate, 7778-77-0; sodium dihydrogen phosphate, 7558-80-7, 7632-05-5; sucrose, 122880-25-5, 57-50-1; trehalose, 99-20-7; water, 7732-18-5; Phosphates; Sucrose, 57-50-1; Trehalose, 99-20-7; Water, 7732-18-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07248741_v21_n9_p1615_Ohtake

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Citas:

---------- APA ----------
Ohtake, S., Schebor, C., Palecek, S.P. & De Pablo, J.J. (2004) . Effect of pH, counter ion, and phosphate concentration on the glass transition temperature of freeze-dried sugar-phosphate mixtures. Pharmaceutical Research, 21(9), 1615-1621.
http://dx.doi.org/10.1023/B:PHAM.0000041456.19377.87
---------- CHICAGO ----------
Ohtake, S., Schebor, C., Palecek, S.P., De Pablo, J.J. "Effect of pH, counter ion, and phosphate concentration on the glass transition temperature of freeze-dried sugar-phosphate mixtures" . Pharmaceutical Research 21, no. 9 (2004) : 1615-1621.
http://dx.doi.org/10.1023/B:PHAM.0000041456.19377.87
---------- MLA ----------
Ohtake, S., Schebor, C., Palecek, S.P., De Pablo, J.J. "Effect of pH, counter ion, and phosphate concentration on the glass transition temperature of freeze-dried sugar-phosphate mixtures" . Pharmaceutical Research, vol. 21, no. 9, 2004, pp. 1615-1621.
http://dx.doi.org/10.1023/B:PHAM.0000041456.19377.87
---------- VANCOUVER ----------
Ohtake, S., Schebor, C., Palecek, S.P., De Pablo, J.J. Effect of pH, counter ion, and phosphate concentration on the glass transition temperature of freeze-dried sugar-phosphate mixtures. Pharm. Res. 2004;21(9):1615-1621.
http://dx.doi.org/10.1023/B:PHAM.0000041456.19377.87