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Abstract:

The bacterioplankton assemblages of eight maritime Antarctic lakes with a wide range of trophic status and geographic span (six lakes from Hope Bay, Antarctic Peninsula and two from Potter Peninsula, King George Island) were described using denaturing gradient gel electrophoresis and band sequencing during two consecutive austral summers (2003-2004). Analyses of the gels identified a total of 230 bands spread across 57 different positions. Among those bands, 14 were shared between lakes from Hope Bay and Potter Peninsula, 17 were observed only in particular lakes, and 17 were registered both years in the same lake. We successfully reamplified and sequenced 43 bands located in 36 different positions belonging to Bacteroidetes, Actinobacteria, Betaproteobacteria and Cyanobacteria. The closest matches for 63% of the sequenced bands were from Antarctic or from other cold environment clones and sequences already in the databases, suggesting the widespread dominance of microbial communities adapted to cold habitats. The results of the multivariate analyses (Cluster Analysis and CCA) indicated that the nutrient status of the lake influences the bacterioplankton assemblages. © 2009 Springer-Verlag.

Registro:

Documento: Artículo
Título:Comparative analysis of bacterioplankton assemblages from maritime Antarctic freshwater lakes with contrasting trophic status
Autor:Schiaffino, M.R.; Unrein, F.; Gasol, J.M.; Farias, M.E.; Estevez, C.; Balagué, V.; Izaguirre, I.
Filiación:Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
CONICET (Consejo Nacional de Investigación Científica y Técnica), Buenos Aires, Argentina
IIB-INTECH, Chascomús, Argentina
Departament de Biología Marina i Oceanografia, Institut de Ciències Del Mar-CSIC, Barcelona, Catalonia, Spain
PROIMI-CONICET (Planta Piloto de Procesos Industriales Microbiológicos), Tucumán, Argentina
Palabras clave:16S rRNA gene; Bacterioplankton; Denaturing gradient gel electrophoresis; Hope Bay (Antarctic Peninsula); Maritime Antarctic lakes; Potter Peninsula (King George Island); bacterioplankton; bacterium; cluster analysis; comparative study; cyanobacterium; dominance; electrokinesis; gel; gene; microbial community; RNA; trophic status; Antarctic Peninsula; Antarctica; Hope Bay; King George Island; Potter Peninsula; South Shetland Islands; West Antarctica; Actinobacteria; Bacteroidetes; Betaproteobacteria; Cyanobacteria
Año:2009
Volumen:32
Número:6
Página de inicio:923
Página de fin:936
DOI: http://dx.doi.org/10.1007/s00300-009-0593-6
Título revista:Polar Biology
Título revista abreviado:Polar Biol.
ISSN:07224060
CODEN:POBID
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07224060_v32_n6_p923_Schiaffino

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Citas:

---------- APA ----------
Schiaffino, M.R., Unrein, F., Gasol, J.M., Farias, M.E., Estevez, C., Balagué, V. & Izaguirre, I. (2009) . Comparative analysis of bacterioplankton assemblages from maritime Antarctic freshwater lakes with contrasting trophic status. Polar Biology, 32(6), 923-936.
http://dx.doi.org/10.1007/s00300-009-0593-6
---------- CHICAGO ----------
Schiaffino, M.R., Unrein, F., Gasol, J.M., Farias, M.E., Estevez, C., Balagué, V., et al. "Comparative analysis of bacterioplankton assemblages from maritime Antarctic freshwater lakes with contrasting trophic status" . Polar Biology 32, no. 6 (2009) : 923-936.
http://dx.doi.org/10.1007/s00300-009-0593-6
---------- MLA ----------
Schiaffino, M.R., Unrein, F., Gasol, J.M., Farias, M.E., Estevez, C., Balagué, V., et al. "Comparative analysis of bacterioplankton assemblages from maritime Antarctic freshwater lakes with contrasting trophic status" . Polar Biology, vol. 32, no. 6, 2009, pp. 923-936.
http://dx.doi.org/10.1007/s00300-009-0593-6
---------- VANCOUVER ----------
Schiaffino, M.R., Unrein, F., Gasol, J.M., Farias, M.E., Estevez, C., Balagué, V., et al. Comparative analysis of bacterioplankton assemblages from maritime Antarctic freshwater lakes with contrasting trophic status. Polar Biol. 2009;32(6):923-936.
http://dx.doi.org/10.1007/s00300-009-0593-6