Artículo

La versión final de este artículo es de uso interno. El editor solo permite incluir en el repositorio el artículo en su versión post-print. Por favor, si usted la posee enviela a
Consulte la política de Acceso Abierto del editor

Abstract:

Two Antarctic marine bacterial strains were isolated, characterised and exposed to solar radiation during summer at Potter Cove (King George Island, South Shetland Islands, Antarctica). The effect of photosynthetically available radiation (PAR), ultraviolet-A (UV-A) and ultraviolet-B radiation (UV-B) on viability was studied using petri dishes covered with cut-off filters and providing the following treatments: dark, PAR, PAR + UV-A, PAR + UV-A + UV-B. UV-A and UV-B radiation have similar negative effects on the viability of the two studied strains under high UV irradiance but different sensitivities were observed under low UV irradiance. The regression analysis showed a logarithmic relationship between loss of viability and UV-B dose, with a threshold effect at 4.2 kJ m-2 (unweighted integrated dose) beyond which there was a > 90% mortality. Hydrogen peroxide in Antarctic seawater under ambient UV radiation reached concentrations up to 1.45 μM. However, assays with one of the bacterial strains showed that there was no detectable effect of hydrogen peroxide over the range 0.5-50 μM, indicating that production of this reactive oxygen species was not directly involved in the observed loss of viability.

Registro:

Documento: Artículo
Título:Effect of solar radiation on two Antarctic marine bacterial strains
Autor:Hernandez, E.; Ferreyra, G.A.; MacCormack, W.P.
Filiación:Instituto Antártico Argentino, Cerrito 1248, Buenos Aires (1010), Argentina
Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:bacterium; photosynthetically active radiation; regression analysis; solar radiation; ultraviolet radiation; Antarctica; Bacteria (microorganisms); Rickettsia sp. PAR
Año:2002
Volumen:25
Número:6
Página de inicio:453
Página de fin:459
Título revista:Polar Biology
Título revista abreviado:Polar Biol.
ISSN:07224060
CODEN:POBID
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07224060_v25_n6_p453_Hernandez

Referencias:

  • Aas, P., Lyons, M.M., Pledger, R., Mitchell, D.L., Jeffrey, W.H., Inhibition of bacterial activities by solar radiation in nearshore waters and the Gulf of Mexico (1996) Aquat Microb Ecol, 11, pp. 229-238
  • Abele-Oeschger, D., Rottgers, R., Tug, H., Dynamics of UV-driven hydrogen peroxide formation on an intertidal sandflat (1997) Limnol Oceanogr, 42, pp. 1406-1415
  • Abele, D., Ferreyra, G.A., Schloss, I., H2O2 accumulation from photochemical production and atmospheric wet deposition in Antarctic coastal and off-shore waters of Potter Cove, King George Island, South Shetland Islands (1999) Antarct Sci, 11, pp. 131-139
  • Anderson, J.G., Toohey, D.W., Brune, W.H., Free radicals within the Antarctic vortex: The role of CFCs in Antarctic ozone loss (1991) Science, 251, pp. 39-46
  • Arana, I., Muela, A., Iriberri, J., Egea, L., Barcina, I., Role of hydrogen peroxide in loss of culturability mediated by visible light in Escherichia coli in a freshwater ecosystem (1992) Appl Environ Microbiol, 58, pp. 3903-3907
  • Arrage, A.A., Phelps, T.J., Benoit, R.E., White, D.C., Survival of subsurface microorganisms exposed to UV radiation and hydrogen peroxide (1993) Appl Environ Microbiol, 59, pp. 3545-3550
  • Arrieta, J.M., Weinbauer, M.G., Herndl, G.J., Interspecific variability in sensitivity to UV radiation and subsequent recovery in selected isolates of marine bacteria (2000) Appl Environ Microbiol, 66, pp. 1468-1473
  • Bader, H., Sturzenegger, V., Hoigné, J., Photometric method for the determination of low concentrations of hydrogen peroxide by the peroxidase catalyzed oxidation of N,N-diethyl-p-phenylenediamine (DPD) (1988) Water Res, 22, pp. 1109-1115
  • Booth, C.R., Morrow, J.H., Coohill, T.P., Cullen, J.J., Frederick, J.E., Häder, D.-P., Holm-Hansen, O., Worrest, R.C., Impacts of solar UVR on aquatic microorganisms (1997) Photochem Photobiol, 65, pp. 252-269
  • Cabrera, S., Lopez, M., Tartarotti, B., Phytoplankton and zooplankton response to ultraviolet radiation in a high altitude Andean lake: Short- versus long term effects (1997) J Plankton Res, 19, pp. 1565-1582
  • Cho, B.C., Azam, F., Major role of bacteria in biogeochemical fluxes in the ocean's interior (1988) Nature, 332, pp. 441-443
  • Cho, B.C., Azam, F., Biogeochemical significance of bacterial biomass in the ocean's euphoric zone (1990) Mar Ecol Prog Res, 63, pp. 253-259
  • Davidson, A.T., The impact of UV-B radiation on marine plankton (1998) Mutation Res, 422, pp. 119-129
  • Davidson, A.T., Marchant, H.J., De la Mare, W.K., Natural UVB exposure changes the species composition of Antarctic phytoplankton in mixed culture (1996) Aquat Microb Ecol, 10, pp. 299-305
  • Delille, D., Bouvy, M., Cahet, G., Short-term variation of bacterioplankton in Antarctic zone: Terre Adelie Area (1988) Microb Ecol, 15, pp. 293-309
  • Doney, S.C., Najjar, R.G., Stewar, S., Photochemistry, mixing and diurnal cycles in the upper ocean (1995) J Mar Res, 53, pp. 341-369
  • Elasri, M.O., Miller, R.V., Study of the response of a biofilm bacterial community to UV radiation (1999) Appl Environ Microbiol, 65, pp. 2025-2031
  • Fernández, R.O., Pizarro, R.A., Lethal effect induced in Pseudomonas aeruginosa exposed to ultraviolet-A radiation (1996) Photochem Photobiol, 64, pp. 334-339
  • Frederick, J.E., Lubin, D., The budget of biologically active ultraviolet radiation in the earth-atmosphere system (1988) J Geophys Res, 93, pp. 3825-3832
  • Helbling, E.W., Marguet, E.R., Villafañe, V.E., Holm-Hansen, O., Bacterioplankton viability in Antarctic waters as affected by solar ultraviolet radiation (1995) Mar Ecol Prog Ser, 166, pp. 293-298
  • Herndl, J.G., Muller-Niklas, G., Frick, J., Major role of ultraviolet-B in controlling bacterioplankton growth in surface layer of the ocean (1993) Nature, 361, pp. 717-719
  • Herndl, G.J., Brugger, A., Hager, S., Kaiser, E., Obernosterer, I., Reitner, B., Slezak, D., Role of ultraviolet-B radiation on bacterioplankton and the availability of dissolved organic matter (1997) Plant Ecol, 128, pp. 42-51
  • Herndl, G.J., Arrieta, J.M., Kaiser, E., Obernosterer, I., Pausz, C., Reitner, B., Role of ultraviolet radiation in aquatic systems: Interaction between mixing processes, photochemistry and microbial activity (2000) Proceedings of the 8th International Symposium on Microbial Ecology, pp. 209-219. , Bell CR, Brylinsky M, Johnson-Green P (eds) Microbial biosystems: New frontiers. Atlantic Canada Society for Microbial Ecology, Halifax, Canada
  • Herrmann, R., The daily changing pattern of hydrogen peroxide in New Zealand surface waters (1996) Environ Toxicol Chem, 15, pp. 652-662
  • Huot, Y., Jeffrey, W.H., Davis, R.F., Cullen, J.J., Damage to DNA in bacterioplankton: A model of damage by ultraviolet radiation and its repair as influenced by vertical mixing (2000) Photochem Photobiol, 72, pp. 62-74
  • Jeffrey, W.H., Pledger, R.J., Aas, P., Hager, S., Coffin, R.B., Von Haven, R., Mitchell, D.L., Diel and depth profiles of DNA photo-damage in bacterioplankton exposed to ambient solar ultraviolet radiation (1996) Mar Ecol Prog Set, 137, pp. 283-291
  • Karentz, D., Lutze, L.H., Evaluation of biologically harmful ultraviolet radiation in Antarctica with a biological dosimeter designed for aquatic environments (1990) Limnol Oceanogr, 35, pp. 549-561
  • Karl, D.M., Microbial processes in the southern oceans (1993) Antarctic Microbiology, pp. 1-63. , Friedman EI (ed). Wiley, New York
  • Kieber, R.J., McDaniel, J., Mopper, K., Photochemical source of biological substrates in sea water: Implication of carbon cycling (1989) Nature, 341, pp. 637-639
  • Kim, D.S., Watanabe, Y., The effect of long wave ultraviolet radiation (UV-A) on the photosynthetic activity of natural populations of freshwater phytoplankton (1993) Ecol Res, 8, pp. 225-234
  • Kim, D.S., Watanabe, Y., Inhibition of growth and photosynthesis of freshwater phytoplankton by ultraviolet A (UV-A) radiation and subsequent recovery from stress (1994) J Plankton Res, 16, pp. 1645-1654
  • Miller, R.V., Jeffrey, W., Mitchell, D., Elasri, M., Bacterial responses to ultraviolet light (1999) ASM News, 65, pp. 535-541
  • Milot-Roy, V., Vincent, W.F., UV radiation effects on photosynthesis: The importance of near-surface thermocline in a subarctic lake (1994) Arch Hydrobiol Beih Ergebn Limnol, 43, pp. 171-184
  • Müller-Niklas, G., Heissenberger, A., Puškaric, S., Herndl, G.J., Ultraviolet-B radiation and bacterial metabolism in coastal waters (1995) Aquat Microb Ecol, 9, pp. 11-116
  • Pirt, S.J., (1985) Death of Cells in Growing Cultures. Principles of Microbe and Cell Cultivation, , Blackwell, London
  • Schloss, I.R., (1997) Escalas Temporo-Espaciales de Variabilidad del Fitoplancton Costero Antátrtico, , PhD Thesis, University of Buenos Aires
  • Sommaruga, R., Oberleiter, A., Psenner, R., Effect of UV radiation on the bacterivory of a heterotrophic nanoflagellate (1996) Appl Environ Microbiol, 62, pp. 4395-4400
  • Sommaruga, R., Oberleiter, A., Herndl, G.J., Psenner, R., Inhibitory effect of solar radiation on thymidine and leucine incorporation by freshwater and marine bacterioplankton (1997) Appl Environ Microbiol, 63, pp. 4178-4184
  • Staehelin, J., Harris, N.R.P., Appenzeller, C., Ebenhard, J., Ozone trends: A review (2001) Rev Geophys, 39, pp. 231-290
  • Tanner, A., The role of bacteria in the cycling of nutrients within the maritime Antarctic environment (1985) Antarctic Nutrient Cycles and Food Webs, pp. 123-127. , Siegfried WR, Condy PR, Laws RM (eds). Springer, Berlin Heidelberg New York
  • Vincent, W.F., Neale, P.J., Mechanisms of UV damage to aquatic organisms (2000) The Effects of UV Radiation in the Marine Environment, pp. 149-156. , Mora SJ de, Demers S, Vernet M (eds). Cambridge University Press, Cambridge
  • Winter, C., Moesender, M.M., Herndl, G.J., Impact of UV radiation on bacterioplankton community composition (2001) Appl Environ Microbiol, 67, pp. 665-672

Citas:

---------- APA ----------
Hernandez, E., Ferreyra, G.A. & MacCormack, W.P. (2002) . Effect of solar radiation on two Antarctic marine bacterial strains. Polar Biology, 25(6), 453-459.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07224060_v25_n6_p453_Hernandez [ ]
---------- CHICAGO ----------
Hernandez, E., Ferreyra, G.A., MacCormack, W.P. "Effect of solar radiation on two Antarctic marine bacterial strains" . Polar Biology 25, no. 6 (2002) : 453-459.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07224060_v25_n6_p453_Hernandez [ ]
---------- MLA ----------
Hernandez, E., Ferreyra, G.A., MacCormack, W.P. "Effect of solar radiation on two Antarctic marine bacterial strains" . Polar Biology, vol. 25, no. 6, 2002, pp. 453-459.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07224060_v25_n6_p453_Hernandez [ ]
---------- VANCOUVER ----------
Hernandez, E., Ferreyra, G.A., MacCormack, W.P. Effect of solar radiation on two Antarctic marine bacterial strains. Polar Biol. 2002;25(6):453-459.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07224060_v25_n6_p453_Hernandez [ ]