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Abstract:

Gonadotropin-releasing hormone (GnRH) is the hypothalamic hormone that regulates the reproductive system by stimulating release of gonadotropins from the anterior pituitary gland. The molecular variants of the reproductive neuropeptide GnRH were characterized from brain tissue of three perciform species from Antarctic waters: Pseudochaenichthys georgianus, Chaenocephalus aceratus, and Notothenia rossi. The study involved reverse phase high-performance liquid chromatography (RP-HPLC) followed by radioimmunoassay (RIA) with two antisera that recognize all GnRH variants already identified: PBL 45 and PBL 49. The results showed that brain extracts of P. georgianus, C. aceratus, and N: rossi contain, like those of other perciform fish, three forms of GnRH likely to be: sbGnRH (seabream GnRH), cGnRH-II (chicken GnRH II) and sGnRH (salmon GnRH). They also showed evidence for the presence of a fourth GnRH variant, chromatographically and immunologically different from the other known forms of the vertebrate hormone. Although final conclusions will require isolation, purification, and sequencing of these molecules, these results offer encouraging possibilities of further advances in the characterization of a multiplicity of GnRH molecular variants.

Registro:

Documento: Artículo
Título:Characterization of brain gonadotropin-releasing hormone (GnRH) molecular variants in brain extracts from different perciform fishes from Antarctic waters
Autor:Miranda, L.A.; Montaner, A.D.; Ansaldo, M.; Affanni, J.M.; Somoza, G.M.
Filiación:Inst. Neurociencia (INEUCI-CONICET), Depto. de Ciencias Biológicas, Universidad de Buenos Aires, Pabellón II, 1428 Buenos Aires, Argentina
Instituto Antártico Argentino, Cerrito 477, Buenos Aires, Argentina
Palabras clave:GnRH fish; Perciformes; Reproduction; Reverse phase HPLC Radioimmunoassay; Chaenocephalus aceratus; chromatography; gonadorelin; high performance liquid chromatography; Notothenia rossi; protein analysis; Pseudochaenichthys georgianus; radioimmunoassay
Año:1999
Volumen:21
Número:2
Página de inicio:122
Página de fin:127
DOI: http://dx.doi.org/10.1007/s003000050342
Título revista:Polar Biology
Título revista abreviado:Polar Biol.
ISSN:07224060
CODEN:POBID
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07224060_v21_n2_p122_Miranda

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Citas:

---------- APA ----------
Miranda, L.A., Montaner, A.D., Ansaldo, M., Affanni, J.M. & Somoza, G.M. (1999) . Characterization of brain gonadotropin-releasing hormone (GnRH) molecular variants in brain extracts from different perciform fishes from Antarctic waters. Polar Biology, 21(2), 122-127.
http://dx.doi.org/10.1007/s003000050342
---------- CHICAGO ----------
Miranda, L.A., Montaner, A.D., Ansaldo, M., Affanni, J.M., Somoza, G.M. "Characterization of brain gonadotropin-releasing hormone (GnRH) molecular variants in brain extracts from different perciform fishes from Antarctic waters" . Polar Biology 21, no. 2 (1999) : 122-127.
http://dx.doi.org/10.1007/s003000050342
---------- MLA ----------
Miranda, L.A., Montaner, A.D., Ansaldo, M., Affanni, J.M., Somoza, G.M. "Characterization of brain gonadotropin-releasing hormone (GnRH) molecular variants in brain extracts from different perciform fishes from Antarctic waters" . Polar Biology, vol. 21, no. 2, 1999, pp. 122-127.
http://dx.doi.org/10.1007/s003000050342
---------- VANCOUVER ----------
Miranda, L.A., Montaner, A.D., Ansaldo, M., Affanni, J.M., Somoza, G.M. Characterization of brain gonadotropin-releasing hormone (GnRH) molecular variants in brain extracts from different perciform fishes from Antarctic waters. Polar Biol. 1999;21(2):122-127.
http://dx.doi.org/10.1007/s003000050342