Artículo

Beracochea, V.C.; Almasia, N.I.; Peluffo, L.; Nahirñak, V.; Hopp, E.H.; Paniego, N.; Heinz, R.A.; Vazquez-Rovere, C.; Lia, V.V. "Sunflower germin-like protein HaGLP1 promotes ROS accumulation and enhances protection against fungal pathogens in transgenic Arabidopsis thaliana" (2015) Plant Cell Reports. 34(10):1717-1733
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Abstract:

Key message: The novel sunflower gene HaGLP1 is the first germin-like protein characterized from the family Asteraceae. It alters the host redox status and confers protection againstSclerotiniasclerotiorumandRhizoctonia solani. Abstract: Germin-like proteins (GLPs) are a large, diverse and ubiquitous family of plant glycoproteins belonging to the Cupin super family. These proteins have been widely studied because of their diverse roles in important plant processes, including defence. The novel sunflower gene HaGLP1 encodes the first germin-like protein characterized from the family Asteraceae. To analyse whether constitutive in vivo expression of the HaGLP1 gene may lead to disease tolerance, we developed transgenic Arabidopsis plants that were molecularly characterized and biologically assessed after inoculation with Sclerotinia sclerotiorum or Rhizoctonia solani.HaGLP1 expression in Arabidopsis plants conferred tolerance to S. sclerotiorum at the first stages of disease and interfered with R. solani infection, thus giving rise to significant protection against the latter. Furthermore, HaGLP1 expression in Arabidopsis plants elevated endogenous ROS levels. HaGLP1-induced tolerance does not appear to be related to a constitutive induction of the plant defence or the ROS-related genes examined here. In conclusion, our data suggest that HaGLP1 is an interesting candidate for the engineering of plants with increased fungal tolerance and that this gene could also be useful for the selection of naturally overexpressing sunflower genotypes for conventional breeding purposes. © 2015, Springer-Verlag Berlin Heidelberg.

Registro:

Documento: Artículo
Título:Sunflower germin-like protein HaGLP1 promotes ROS accumulation and enhances protection against fungal pathogens in transgenic Arabidopsis thaliana
Autor:Beracochea, V.C.; Almasia, N.I.; Peluffo, L.; Nahirñak, V.; Hopp, E.H.; Paniego, N.; Heinz, R.A.; Vazquez-Rovere, C.; Lia, V.V.
Filiación:Instituto de Biotecnología, CICVyA, Instituto Nacional de Tecnología Agropecuaria (INTA)-Castelar, Dr. Nicolás Repetto y De Los Reseros S/Nº (B1686IGC) Hurlingham, Buenos Aires, Provincia De Buenos Aires, Argentina
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, (EHA1428), Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917 (C1033AAJ), Ciudad Autónoma De Buenos Aires, Buenos Aires, Argentina
Palabras clave:Arabidopsis thaliana; Germin-like protein; Rhizoctonia solani; ROS accumulation; Sclerotinia sclerotiorum; Sunflower; germin; glycoprotein; reactive oxygen metabolite; vegetable protein; Arabidopsis; Ascomycetes; gene expression regulation; genetics; metabolism; microbiology; physiology; plant disease; sunflower; transgenic plant; Arabidopsis; Ascomycota; Gene Expression Regulation, Plant; Glycoproteins; Helianthus; Plant Diseases; Plant Proteins; Plants, Genetically Modified; Reactive Oxygen Species
Año:2015
Volumen:34
Número:10
Página de inicio:1717
Página de fin:1733
DOI: http://dx.doi.org/10.1007/s00299-015-1819-4
Título revista:Plant Cell Reports
Título revista abreviado:Plant Cell Rep.
ISSN:07217714
CODEN:PCRPD
CAS:germin; Glycoproteins; Plant Proteins; Reactive Oxygen Species
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07217714_v34_n10_p1717_Beracochea

Referencias:

  • Almasia, N.I., Estudio genómico y funcional del péptido antimicrobiano SNAKIN-1 (2009) Ph.D, , Dissertation: Universidad de Buenos Aires
  • Almasia, N.I., Bazzini, A., Hopp, H.E., Vazquez-Rovere, C., Overexpression of snakin-1 gene enhances resistance to Rhizoctonia solani and Erwinia carotovora in transgenic potato plants (2008) Mol Plant Pathol, 9, pp. 329-338. , COI: 1:CAS:528:DC%2BD1cXmvFSiu7w%3D, PID: 18705874
  • Bernier, F., Berna, A., Germins and germin-like proteins: plant do-all proteins. But what do they do exactly? (2001) Plant Physiol Biochem, 39, pp. 545-554. , COI: 1:CAS:528:DC%2BD3MXlslGls74%3D
  • Blom, N., Gammeltoft, S., Brunak, S., Sequence and structure-based prediction of eukaryotic protein phosphorylation sites (1999) J Mol Biol, 294, pp. 1351-1362. , COI: 1:CAS:528:DyaK1MXnvFaqsrg%3D, PID: 10600390
  • Boland, G.J., Hall, R., Index of plant hosts of Sclerotinia sclerotiorum (1994) Can J Plant Pathol, 16, pp. 93-108
  • Canut, H., Carrasco, A., Galaud, J.P., Cassan, C., Bouyssou, H., Vita, N., Ferrara, P., Pont-Lezica, R., High affinity RGD-binding sites at the plasma membrane of Arabidopsis thaliana links the cell wall (1998) Plant J, 16, pp. 63-71. , COI: 1:CAS:528:DyaK1cXntFOnsLc%3D, PID: 9807828
  • Carter, C., Thornburg, R.W., Germin-like proteins : structure, phylogeny, and function (1999) J Plant Biol, 42, pp. 97-108. , COI: 1:CAS:528:DyaK1MXltVKhsLk%3D
  • Carter, C., Thornburg, R.W., Tobacco nectarin I. Purification and characterization as a germin-like, manganese superoxide dismutase implicated in the defense of floral reproductive tissues (2000) J Biol Chem, 275, pp. 36726-36733. , COI: 1:CAS:528:DC%2BD3cXoslWmu7k%3D, PID: 10952990
  • Carter, C., Graham, R.A., Thornburg, R.W., Arabidopsis thaliana contains a large family of germin-like proteins: characterization of cDNA and genomic sequences encoding 12 unique family members (1998) Plant Mol Biol, 38, pp. 929-943. , COI: 1:CAS:528:DyaK1MXksVaqtw%3D%3D, PID: 9869400
  • Cessna, S.G., Sears, V.E., Dickman, M.B., Low, P.S., Oxalic acid, a pathogenicity factor for Sclerotinia sclerotiorum, suppresses the oxidative burst of the host plant (2000) Plant Cell, 12, pp. 2191-2200. , COI: 1:CAS:528:DC%2BD3MXos1Kn, PID: 11090218
  • Chauhan, J.S., Rao, A., Raghava, G.P.S., In silico platform for prediction of N-, O- and C-glycosites in eukaryotic protein sequences (2013) PLoS ONE, 8, p. e67008. , COI: 1:CAS:528:DC%2BC3sXhtFahsrnN, PID: 23840574
  • Christensen, A.B., Thordal-Christensen, H., Zimmermann, G., Gjetting, T., Lyngkjaer, M.F., Dudler, R., Schweizer, P., The germin-like protein GLP4 exhibits superoxide dismutase activity and is an important component of quantitative resistance in wheat and barley (2004) Mol Plant Microbe Interact, 17, pp. 109-117. , COI: 1:CAS:528:DC%2BD2cXivVSlt74%3D, PID: 14714874
  • Custers, J.H.H.V., Harrison, S.J., Sela-Buurlage, M.B., van Deventer, E., Lageweg, W., Howe, P.W., van der Meijs, P.J., Stuiver, M.H., Isolation and characterisation of a class of carbohydrate oxidases from higher plants, with a role in active defence (2004) Plant J, 39, pp. 147-160. , COI: 1:CAS:528:DC%2BD2cXntFGrtrY%3D, PID: 15225281
  • Dai, F., Xu, T., Wolf, G.A., He, Z., Physiological and Molecular Features of the Pathosystem Arabidopsis thaliana L. -Sclerotinia sclerotiorum Libert (2006) J Integr Plant Biol, 48, pp. 44-52. , COI: 1:CAS:528:DC%2BD28Xpt12itg%3D%3D
  • Davidson, R.M., Reeves, P.A., Manosalva, P.M., Leach, J.E., Germins: a diverse protein family important for crop improvement (2009) Plant Sci, 177, pp. 499-510. , COI: 1:CAS:528:DC%2BD1MXht1OrurrK
  • Dezar, C.A., Gago, G.M., Gonzalez, D.H., Chan, R.L., Hahb-4, a sunflower homeobox-leucine zipper gene, is a developmental regulator and confers drought tolerance to Arabidopsis thaliana plants (2005) Transgenic Res, 14, pp. 429-440. , COI: 1:CAS:528:DC%2BD2MXmvVWqtb4%3D, PID: 16201409
  • Dezar, C.A., Giacomelli, J.I., Manavella, P.A., Ré, D.A., Alves-Ferreira, M., Baldwin, I.T., Bonaventure, G., Chan, R.L., HAHB10, a sunflower HD-Zip II transcription factor, participates in the induction of flowering and in the control of phytohormone-mediated responses to biotic stress (2011) J Exp Bot, 62, pp. 1061-1076. , COI: 1:CAS:528:DC%2BC3MXhtVektLk%3D, PID: 21030388
  • Di Rienzo, J.A., (2009) fgstatistics version 2009, , Accessed November 2014
  • Di Rienzo, J.A., Guzman, A.W., Casanoves, F., A multiple-comparisons method based on the distribution of the root node distance of a binary tree (2002) J Agric Biol Environ Stat, 7, pp. 129-142
  • (2013) Infostat version 2013, , http://www.infostat.com.ar/Accessed, October 2013
  • Donaldson, P.A., Anderson, T., Lane, B.G., Davidson, A.L., Simmonds, D.H., Soybean plants expressing an active oligomeric oxalate oxidase from the wheat gf-2.8 (germin) gene are resistant to the oxalate-secreting pathogen Sclerotina sclerotiorum (2001) Physiol Mol Plant Pathol, 59, pp. 297-307. , COI: 1:CAS:528:DC%2BD38Xht1Gns74%3D
  • Dong, X., Ji, R., Guo, X., Foster, S.J., Chen, H., Dong, C., Liu, Y., Liu, S., Expressing a gene encoding wheat oxalate oxidase enhances resistance to Sclerotinia sclerotiorum in oilseed rape (Brassica napus) (2008) Planta, 228, pp. 331-340. , COI: 1:CAS:528:DC%2BD1cXmvV2ru70%3D, PID: 18446363
  • Druka, A., Kudrna, D., Kannangara, C.G., von Wettstein, D., Kleinhofs, A., Physical and genetic mapping of barley (Hordeum vulgare) germin-like cDNAs (2002) Proc Natl Acad Sci U S A, 99, pp. 850-855. , COI: 1:CAS:528:DC%2BD38Xht1Wis7Y%3D, PID: 11792854
  • Dunwell, J.M., Cupins: a new superfamily of functionally diverse proteins that include germins and plant storage proteins (1998) Biotechnol Genet Eng Rev, 15, pp. 1-32. , COI: 1:CAS:528:DyaK1cXksFemsrk%3D, PID: 9573603
  • Dunwell, J.M., Gibbings, J.G., Mahmood, T., Saqlan Naqvi, S.M., Germin and germin-like proteins: evolution, structure, and function (2008) CRC Crit Rev Plant Sci, 27, pp. 342-375. , COI: 1:CAS:528:DC%2BD1cXhtFKmurnP
  • Ehrenbolger, G.F., Beracochea, V., Pelufo, L., Almasia, N.I., Vazquez Rovere, C., Hopp, H.E., Paniego, N.B., Lia, V.V., Sunflower germin-like proteins: evolution, gene structure and functional characterization (2012) Proc. 18th Int. Sunflower Conf. ASAGIR, pp. 586-592. , Mar del Plata, Argentina
  • Fernández, P., Paniego, N., Lew, S., Hopp, H.E., Heinz, R.A., Differential representation of sunflower ESTs in enriched organ-specific cDNA libraries in a small scale sequencing project (2003) BMC Genom, 4, p. 40
  • Foley, R.C., Gleason, C.A., Anderson, J.P., Hamann, T., Singh, K.B., Genetic and Genomic Analysis of Rhizoctonia solani Interactions with Arabidopsis; Evidence of Resistance Mediated through NADPH Oxidases (2013) PLoS One, 8, p. e56814. , COI: 1:CAS:528:DC%2BC3sXjslyksLg%3D, PID: 23451091
  • Frohman, M.A., Dush, M.K., Martin, G.R., Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer (1988) Proc Natl Acad Sci U S A, 85, pp. 8998-9002. , COI: 1:CAS:528:DyaL1MXntVCmtQ%3D%3D, PID: 2461560
  • Gee, E.P.S., Ingber, D.E., Stultz, C.M., Fibronectin unfolding revisited: modeling cell traction-mediated unfolding of the tenth type-III repeat (2008) PLoS One, 3, p. e2373. , PID: 19020673
  • Godfrey, D., Able, A.J., Dry, I.B., Induction of a grapevine germin-like protein (VvGLP3)gene is closely linked to the site of Erysiphe necator infection: a possible role in defense? (2007) Mol Plant Microbe Interact, 20, pp. 1112-1125. , COI: 1:CAS:528:DC%2BD2sXpsVGkur8%3D, PID: 17849714
  • Godoy, G., Steadman, J.R., Dickman, M.B., Dam, R., Use of mutants to demonstrate the role of oxalic acid in pathogenicity of Sclerotinia sclerotiorum on Phaseolus vulgaris (1990) Physiol Mol Plant Pathol, 37, pp. 179-191. , COI: 1:CAS:528:DyaK3MXhtFylu7Y%3D
  • Guan, C., Ji, J., Jin, C., Wang, G., Li, X., Guan, W., Expression of cholera toxin B subunit lumbrokinase in edible sunflower seeds-the use of transmucosal carrier to enhance its fusion protein’s effect on protection of rats and mice against thrombosis (2014) Biotechnol Prog, 30, pp. 1029-1039. , COI: 1:CAS:528:DC%2BC2cXhslSrsr3K, PID: 25080206
  • Gucciardo, S., Wisniewski, J.-P., Brewin, N.J., Bornemann, S., A germin-like protein with superoxide dismutase activity in pea nodules with high protein sequence identity to a putative rhicadhesin receptor (2007) J Exp Bot, 58, pp. 1161-1171. , COI: 1:CAS:528:DC%2BD2sXktlWjsLY%3D, PID: 17244628
  • Guo, X., Stotz, H.U., Defense against Sclerotinia sclerotiorum in Arabidopsis is dependent on jasmonic acid, salicylic acid, and ethylene signaling (2007) Mol Plant Microbe Interact, 20, pp. 1384-1395. , COI: 1:CAS:528:DC%2BD2sXht1WksbzF, PID: 17977150
  • Hall, T.A., BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT (1999) Nucleic Acids Symp Ser, 41, pp. 95-98. , COI: 1:CAS:528:DC%2BD3cXhtVyjs7Y%3D
  • Hu, X., Bidney, D.L., Yalpani, N., Duvick, J.P., Crasta, O., Folkerts, O., Lu, G., Overexpression of a gene encoding hydrogen peroxide- generating oxalate oxidase evokes defense responses in sunflower 1 (2003) Plant Physiol, 133, pp. 170-181. , COI: 1:CAS:528:DC%2BD3sXntlaitLc%3D, PID: 12970484
  • Hu, Y., Dong, Q., Yu, D., Arabidopsis WRKY46 coordinates with WRKY70 and WRKY53 in basal resistance against pathogen Pseudomonas syringae (2012) Plant Sci, 185-186, pp. 288-297. , PID: 22325892
  • Karimi, M., Inzé, D., Depicker, A., GATEWAY vectors for Agrobacterium-mediated plant transformation (2002) Trends Plant Sci, 7, pp. 193-195. , COI: 1:CAS:528:DC%2BD38Xjtl2ktrk%3D, PID: 11992820
  • Knecht, K., Seyffarth, M., Desel, C., Thurau, T., Sherameti, I., Lou, B., Oelmüller, R., Cai, D., Expression of BvGLP-1 encoding a germin-like protein from sugar beet in Arabidopsis thaliana leads to resistance against phytopathogenic fungi (2010) Mol Plant Microbe Interact, 23, pp. 446-457. , COI: 1:CAS:528:DC%2BC3cXjslemu7k%3D, PID: 20192832
  • Kyte, J., Doolittle, R.F., A simple method for displaying the hydropathic character of a protein (1982) J Mol Biol, 157, pp. 105-132. , COI: 1:CAS:528:DyaL38Xks1yjtro%3D, PID: 7108955
  • Lane, B.G., Oxalate, germin, and the extracellular matrix of higher plants (1994) FASEB J, 8, pp. 294-301. , COI: 1:CAS:528:DyaK2cXis1GksLY%3D, PID: 8143935
  • Legendre, L., Rueter, S., Heinstein, P.F., Low, P.S., Characterization of the Oligogalacturonide-Induced Oxidative Burst in Cultured Soybean (Glycine max) Cells (1993) Plant Physiol, 102, pp. 233-240. , COI: 1:CAS:528:DyaK3sXks1yhs78%3D, PID: 12231814
  • León-Galván, F., de Jesús, J.-R.A., Torres-Pacheco, I., Barba de la Rosa, A.P., Guevara-Olvera, L., González-Chavira, M.M., Ocampo-Velazquez, R.V., Guevara-González, R.G., A germin-like protein gene (CchGLP) of Capsicum chinense Jacq. Is induced during incompatible interactions and displays Mn-superoxide dismutase activity (2011) Int J Mol Sci, 12, pp. 7301-7313. , PID: 22174599
  • Liu, B., Zhang, S., Zhu, X., Yang, Q., Wu, S., Mei, M., Mauleon, R., Leung, H., Candidate defense genes as predictors of quantitative blast resistance in rice (2004) Mol Plant Microbe Interact, 17, pp. 1146-1152. , COI: 1:CAS:528:DC%2BD2cXnvVaru70%3D, PID: 15497407
  • Livingstone, D.M., Hampton, J.L., Phipps, P.M., Grabau, E.A., Enhancing resistance to Sclerotinia minor in peanut by expressing a barley oxalate oxidase gene (2005) Plant Physiol, 137, pp. 1354-1362. , COI: 1:CAS:528:DC%2BD2MXjslaqu74%3D, PID: 15778458
  • Manavella, P.A., Dezar, C.A., Bonaventure, G., Baldwin, I.T., Chan, R.L., HAHB4, a sunflower HD-Zip protein, integrates signals from the jasmonic acid and ethylene pathways during wounding and biotic stress responses (2008) Plant J, 56, pp. 376-388. , COI: 1:CAS:528:DC%2BD1cXhsVGmtbrJ, PID: 18643970
  • Manavella, P.A., Chan, R.L., Transient transformation of sunflower leaf discs via an Agrobacterium-mediated method: applications for gene expression and silencing studies (2009) Nat Protoc, 4, pp. 1699-1707. , COI: 1:CAS:528:DC%2BD1MXhtlOqsLrK, PID: 19876029
  • Manavella, P.A., Arce, A.L., Dezar, C.A., Bitton, F., Renou, J.-P., Crespi, M., Chan, R.L., Cross-talk between ethylene and drought signalling pathways is mediated by the sunflower Hahb-4 transcription factor (2006) Plant J, 48, pp. 125-137. , COI: 1:CAS:528:DC%2BD28XhtFalsb7M, PID: 16972869
  • Manning, V.A., Hamilton, S.M., Karplus, P.A., Ciuffetti, L.M., The Arg-Gly-Asp-containing, solvent-exposed loop of Ptr ToxA is required for internalization (2008) Mol Plant Microbe Interact, 21, pp. 315-325. , COI: 1:CAS:528:DC%2BD1cXitlygur4%3D, PID: 18257681
  • Manosalva, P.M., Davidson, R.M., Liu, B., Zhu, X., Hulbert, S.H., Leung, H., Leach, J.E., A germin-like protein gene family functions as a complex quantitative trait locus conferring broad-spectrum disease resistance in rice (2009) Plant Physiol, 149, pp. 286-296. , COI: 1:CAS:528:DC%2BD1MXjt1Wqtrk%3D, PID: 19011003
  • Marino, D., Dunand, C., Puppo, A., Pauly, N., A burst of plant NADPH oxidases (2012) Trends Plant Sci, 17, pp. 9-15. , COI: 1:CAS:528:DC%2BC38XptVSnsQ%3D%3D, PID: 22037416
  • Moretti, S., Armougom, F., Wallace, I.M., Higgins, D.G., Jongeneel, C.V., Notredame, C., The M-Coffee web server: a meta-method for computing multiple sequence alignments by combining alternative alignment methods (2007) Nucleic Acids Res, pp. W645-W648
  • Pan, H.-Y., Whittaker, M.M., Bouveret, R., Berna, A., Bernier, F., Whittaker, J.W., Characterization of wheat germin (oxalate oxidase) expressed by Pichia pastoris (2007) Biochem Biophys Res Commun, 356, pp. 925-929. , COI: 1:CAS:528:DC%2BD2sXjvFOmt74%3D, PID: 17399681
  • Patnaik, B.B., Kim, D.H., Oh, S.H., Song, Y.S., Chanh, N.D.M., Kim, J.S., Jung, W.J., Han, Y.S., Molecular cloning and characterization of novel Morus alba germin-like protein gene which encodes for a silkworm gut digestion-resistant antimicrobial protein (2012) PLoS One, 7, p. e50900. , COI: 1:CAS:528:DC%2BC3sXktVSltA%3D%3D, PID: 23284650
  • Peleg-Grossman, S., Melamed-Book, N., Cohen, G., Levine, A., Cytoplasmic H2O2 prevents translocation of NPR1 to the nucleus and inhibits the induction of PR genes in Arabidopsis (2010) Plant Signal Behav, 5, pp. 1401-1406. , COI: 1:CAS:528:DC%2BC3MXhs1Gqtr3F, PID: 21051935
  • Peluffo, L., Caracterizacion de los mecanismos de defensa a Sclerotinia sclerotiorum agente causal de la podredumbre humeda de capitulo de girasol a traves del estudio de perfiles metabolicos y transcripcionales (2010) Ph.D, , Dissertation: Universidad de Buenos Aires
  • Perchepied, L., Balagué, C., Riou, C., Claudel-Renard, C., Rivière, N., Grezes-Besset, B., Roby, D., Lumière, F., Nitric oxide participates in the complex interplay of defense-related signaling pathways controlling disease resistance to Sclerotinia sclerotiorum in Arabidopsis thaliana (2010) Mol Plant Microbe Interact, 23, pp. 846-860. , COI: 1:CAS:528:DC%2BC3cXnvVart74%3D, PID: 20521948
  • Personat, J.-M., Tejedor-Cano, J., Prieto-Dapena, P., Almoguera, C., Jordano, J., Cooverexpression of two Heat Shock Factors results in enhanced seed longevity and in synergistic effects on seedling tolerance to severe dehydration and oxidative stress (2014) BMC Plant Biol, 14, p. 56. , PID: 24593798
  • Petersen, T.N., Brunak, S., von Heijne, G., Nielsen, H., SignalP 4.0: discriminating signal peptides from transmembrane regions (2011) Nat Methods, 8, pp. 785-786. , COI: 1:CAS:528:DC%2BC3MXht1CrtrbL, PID: 21959131
  • Pfaffl, M.W., A new mathematical model for relative quantification in real-time RT-PCR (2001) Nucleic Acids Res, 29 (9), p. e45. , COI: 1:STN:280:DC%2BD38nis12jtw%3D%3D, PID: 11328886
  • Radonic, L.M., Zimmermann, J.M., Zavallo, D., Lopez, N., Lopez Bilbao, M., Rooting in Km selective media as efficient in vitro selection method for sunflower genetic transformation (2006) Electron J Biotechnol
  • Ramakers, C., Ruijter, J.M., Deprez, R.H.L., Moorman, A.F.M., Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data (2003) Neurosci Lett, 339, pp. 62-66. , COI: 1:CAS:528:DC%2BD3sXhs1Kks70%3D, PID: 12618301
  • Rietz, S., Bernsdorff, F.E.M., Cai, D., Members of the germin-like protein family in Brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis of Sclerotinia sclerotiorum (2012) J Exp Bot, 63, pp. 5507-5519. , COI: 1:CAS:528:DC%2BC38XhsVSmsrvF, PID: 22888126
  • Schwartz, M.A., Transmembrane signalling by integrins (1992) Trends Cell Biol, 2, pp. 304-308. , COI: 1:CAS:528:DyaK3sXitV2rsrs%3D, PID: 14731926
  • Shetty, N.P., Mehrabi, R., Lütken, H., Haldrup, A., Kema, G.H.J., Collinge, D.B., Jørgensen, H.J.L., Role of hydrogen peroxide during the interaction between the hemibiotrophic fungal pathogen Septoria tritici and wheat (2007) New Phytol, 174, pp. 637-647. , COI: 1:CAS:528:DC%2BD2sXmsFOktbo%3D, PID: 17447918
  • Shetty, N.P., Jørgensen, H.J.L., Jensen, J.D., Collinge, D.B., Shetty, H.S., Roles of reactive oxygen species in interactions between plants and pathogens (2008) Eur J Plant Pathol, 121, pp. 267-280. , COI: 1:CAS:528:DC%2BD1cXmtValt74%3D
  • Sujatha, M., Vijay, S., Vasavi, S., Veera Reddy, P., Chander Rao, S., Agrobacterium-mediated transformation of cotyledons of mature seeds of multiple genotypes of sunflower (Helianthus annuus L.) (2012) Plant Cell, Tissue Organ Cult, 110, pp. 275-287
  • Walz, A., Zingen-Sell, I., Loeffler, M., Sauer, M., Expression of an oxalate oxidase gene in tomato and severity of disease caused by Botrytis cinerea and Sclerotinia sclerotiorum (2008) Plant Pathol, 57, pp. 453-458. , COI: 1:CAS:528:DC%2BD1cXnvVOksbc%3D
  • Wang, Z., Tan, X., Zhang, Z., Gu, S., Li, G., Shi, H., Defense to Sclerotinia sclerotiorum in oilseed rape is associated with the sequential activations of salicylic acid signaling and jasmonic acid signaling (2012) Plant Sci, 184, pp. 75-82. , COI: 1:CAS:528:DC%2BC38XhsFSisLo%3D, PID: 22284712
  • Wang, T., Chen, X., Zhu, F., Li, H., Li, L., Yang, Q., Chi, X., Liang, X., Characterization of peanut germin-like proteins, AhGLPs in plant development and defense (2013) PLoS ONE, 8, p. e61722. , COI: 1:CAS:528:DC%2BC3sXntVKrtLs%3D, PID: 23626720
  • Wei, Y., Zhang, Z., Andersen, C.H., Schmelzer, E., Gregersen, P.L., Collinge, D.B., Smedegaard-Petersen, V., Thordal-Christensen, H., An epidermis/papilla-specific oxalate oxidase like protein in the defence response of barley attacked by the powdery mildew fungus (1998) Plant Mol Biol, 36, pp. 101-112. , COI: 1:CAS:528:DyaK1cXht1akt70%3D, PID: 9484466
  • Weigel, D., Glazebrook, J., (2006) In planta transformation of Arabidopsis, , CSH Protoc 006:pdb.prot4668–
  • Williams, B., Kabbage, M., Kim, H.J., Britt, R., Dickman, M.B., Tipping the balance: sclerotinia sclerotiorum secreted oxalic acid suppresses host defenses by manipulating the host redox environment (2011) PLoS Pathog, 7, p. e1002107. , COI: 1:CAS:528:DC%2BC3MXovVygsbc%3D, PID: 21738471
  • Wohlgemuth, H., Mittelstrass, K., Kschieschan, S., Bender, J., Weigel, H.-J., Overmyer, K., Kangasjarvi, J., Langebartels, C., Activation of an oxidative burst is a general feature of sensitive plants exposed to the air pollutant ozone (2002) Plant Cell Environ, 25, pp. 717-726. , COI: 1:CAS:528:DC%2BD38XkvFSju7g%3D
  • Yamahara, T., Shiono, T., Suzuki, T., Tanaka, K., Takio, S., Sato, K., Yamazaki, S., Satoh, T., Isolation of a Germin-like Protein with Manganese Superoxide Dismutase activity from cells of a moss, Barbula unguiculata (1999) J Biol Chem, 274, pp. 33274-33278. , COI: 1:CAS:528:DyaK1MXns1aqtbc%3D, PID: 10559202
  • Zhou, F., Zhang, Z., Gregersen, P.L., Mikkelsen, J.D., De, N.E., Collinge, D.B., Thordal-Christensen, H., Molecular characterization of the oxalate oxidase involved in the response of barley to the powdery mildew fungus 1 (1998) Plant Physiol, 117, pp. 33-41. , COI: 1:CAS:528:DyaK1cXjsFSksLs%3D, PID: 9576772
  • Zimmermann, G., Bäumlein, H., Mock, H.-P., Himmelbach, A., Schweizer, P., The multigene family encoding germin-like proteins of barley. Regulation and function in Basal host resistance (2006) Plant Physiol, 142, pp. 181-192. , COI: 1:CAS:528:DC%2BD28XpvVKhsrg%3D, PID: 16844832
  • Zubrzycki, J., Estudio de la resistencia a Sclerotinia sclerotiorum en líneas endocriadas de girasol (2015) Ph.D, , Dissertation: Universidad de Buenos Aires

Citas:

---------- APA ----------
Beracochea, V.C., Almasia, N.I., Peluffo, L., Nahirñak, V., Hopp, E.H., Paniego, N., Heinz, R.A.,..., Lia, V.V. (2015) . Sunflower germin-like protein HaGLP1 promotes ROS accumulation and enhances protection against fungal pathogens in transgenic Arabidopsis thaliana. Plant Cell Reports, 34(10), 1717-1733.
http://dx.doi.org/10.1007/s00299-015-1819-4
---------- CHICAGO ----------
Beracochea, V.C., Almasia, N.I., Peluffo, L., Nahirñak, V., Hopp, E.H., Paniego, N., et al. "Sunflower germin-like protein HaGLP1 promotes ROS accumulation and enhances protection against fungal pathogens in transgenic Arabidopsis thaliana" . Plant Cell Reports 34, no. 10 (2015) : 1717-1733.
http://dx.doi.org/10.1007/s00299-015-1819-4
---------- MLA ----------
Beracochea, V.C., Almasia, N.I., Peluffo, L., Nahirñak, V., Hopp, E.H., Paniego, N., et al. "Sunflower germin-like protein HaGLP1 promotes ROS accumulation and enhances protection against fungal pathogens in transgenic Arabidopsis thaliana" . Plant Cell Reports, vol. 34, no. 10, 2015, pp. 1717-1733.
http://dx.doi.org/10.1007/s00299-015-1819-4
---------- VANCOUVER ----------
Beracochea, V.C., Almasia, N.I., Peluffo, L., Nahirñak, V., Hopp, E.H., Paniego, N., et al. Sunflower germin-like protein HaGLP1 promotes ROS accumulation and enhances protection against fungal pathogens in transgenic Arabidopsis thaliana. Plant Cell Rep. 2015;34(10):1717-1733.
http://dx.doi.org/10.1007/s00299-015-1819-4