Artículo

Burrieza, H.P.; López-Fernández, M.P.; Chiquieri, T.B.; Silveira, V.; Maldonado, S. "Accumulation pattern of dehydrins during sugarcane (var. SP80.3280) somatic embryogenesis" (2012) Plant Cell Reports. 31(12):2139-2149
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Abstract:

The objective of the present study was to determine dehydrin protein levels in sugarcane var. SP80-3280 during somatic embryogenesis. Dehydrins from embryogenic and non-embryogenic cell cultures were analyzed using western blot and in situ immunolocalization microscopy. Both techniques employ antibodies raised against a highly conserved lysine-rich 15-amino acid sequence termed the K-domain, which is extensively used to recognize proteins immunologically related to the dehydrin family. In embryogenic cultures, western blot analysis of the heat-stable protein fraction revealed eleven major bands ranging from 52 to 17 kDa. They were already visible on the first days, gradually increasing until reaching peak values around day 14, when organogenesis begins, to later decrease in concurrence with the appearance of green plantlets (around day 28). These fluctuations indicate that this pattern of accumulation is under developmental control. Dehydrins were mainly immunolocalized in the nuclei. A phosphatase treatment of protein extracts caused a mobility shift of the 52, 49, and 43 kDa dehydrin bands suggesting a putative modulation mechanism based on protein phosphorylation. In sugarcane embryogenic cultures, presence of dehydrins is a novel finding. Dehydrins were absent in non-embryogenic cultures. The novel findings regarding accumulation, nuclear localization, and phosphorylation of dehydrins provide a starting point for further research on the role of these proteins in the induction and/or maintenance of embryogenesis. Key message The novel findings regarding accumulation, nuclear localization, and phosphorylation of dehydrins provide a starting point for further research on the role of these proteins in the induction and/or maintenance of embryogenesis. © 2012 Springer-Verlag.

Registro:

Documento: Artículo
Título:Accumulation pattern of dehydrins during sugarcane (var. SP80.3280) somatic embryogenesis
Autor:Burrieza, H.P.; López-Fernández, M.P.; Chiquieri, T.B.; Silveira, V.; Maldonado, S.
Filiación:Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2 Ciudad Universitaria, C1428EGA Buenos Aires, Argentina
Laboratório de Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, RJ, Brazil
Palabras clave:Dehydrin in situ immunolocalization; Dehydrin nuclear localization; Dehydrin phosphorylation; Dehydrin western blot; Dehydrins; Embryogenic competence; Somatic embryogenesis; Sugarcane var. SP80-3280; dehydrin proteins, plant; lysine; vegetable protein; amino acid sequence; article; cell nucleus; growth, development and aging; histology; immunohistochemistry; meristem; metabolism; methodology; phosphorylation; plant seed; protein stability; protein tertiary structure; somatic embryogenesis; sugarcane; transmission electron microscopy; ultrastructure; Western blotting; Amino Acid Sequence; Blotting, Western; Cell Nucleus; Immunohistochemistry; Lysine; Meristem; Microscopy, Electron, Transmission; Phosphorylation; Plant Proteins; Plant Somatic Embryogenesis Techniques; Protein Stability; Protein Structure, Tertiary; Saccharum; Seeds
Año:2012
Volumen:31
Número:12
Página de inicio:2139
Página de fin:2149
DOI: http://dx.doi.org/10.1007/s00299-012-1323-z
Título revista:Plant Cell Reports
Título revista abreviado:Plant Cell Rep.
ISSN:07217714
CODEN:PCRPD
CAS:lysine, 56-87-1, 6899-06-5, 70-54-2; Lysine, 56-87-1; Plant Proteins; dehydrin proteins, plant, 134711-03-8
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07217714_v31_n12_p2139_Burrieza

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Citas:

---------- APA ----------
Burrieza, H.P., López-Fernández, M.P., Chiquieri, T.B., Silveira, V. & Maldonado, S. (2012) . Accumulation pattern of dehydrins during sugarcane (var. SP80.3280) somatic embryogenesis. Plant Cell Reports, 31(12), 2139-2149.
http://dx.doi.org/10.1007/s00299-012-1323-z
---------- CHICAGO ----------
Burrieza, H.P., López-Fernández, M.P., Chiquieri, T.B., Silveira, V., Maldonado, S. "Accumulation pattern of dehydrins during sugarcane (var. SP80.3280) somatic embryogenesis" . Plant Cell Reports 31, no. 12 (2012) : 2139-2149.
http://dx.doi.org/10.1007/s00299-012-1323-z
---------- MLA ----------
Burrieza, H.P., López-Fernández, M.P., Chiquieri, T.B., Silveira, V., Maldonado, S. "Accumulation pattern of dehydrins during sugarcane (var. SP80.3280) somatic embryogenesis" . Plant Cell Reports, vol. 31, no. 12, 2012, pp. 2139-2149.
http://dx.doi.org/10.1007/s00299-012-1323-z
---------- VANCOUVER ----------
Burrieza, H.P., López-Fernández, M.P., Chiquieri, T.B., Silveira, V., Maldonado, S. Accumulation pattern of dehydrins during sugarcane (var. SP80.3280) somatic embryogenesis. Plant Cell Rep. 2012;31(12):2139-2149.
http://dx.doi.org/10.1007/s00299-012-1323-z