Abstract:
Treatment of shoot apices of the marubakaido apple rootstock with a fluoro derivative of 28-homoethylcastasterone (5F-HCTS) in the range 100-10,000 ng per shoot increased the apple rootstock's multiplication rate (MR). A dose of 500 ng 5F-HCTS in 5 μl ethanol, per shoot, resulted in a significant (P=0.05) 112% increase in MR compared to shoots treated with 5 μl of 95% ethanol when the shoots were grown in culture medium enriched with 2.2 μM N6-benzyladenine. This increase in MR was due to: (1) a 15% increase in the number of main branches; (2) a 238% increase in the number of primary lateral branches; (3) a 250% increase in the number of secondary lateral branches. These results show that shoot proliferation induced by the fluoro derivative of 28-homocastasterone is an effective method to enhance the in vitro multiplication rate in Malus prunifolia.
Registro:
Documento: |
Artículo
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Título: | Brassinosteroid-driven enhancement of the in vitro multiplication rate for the marubakaido apple rootstock [Malus prunifolia (Willd.) Borkh] |
Autor: | Schaefer, S.; Medeiro, S.; Ramírez, J.; Galagovsky, L.; Pereira-Netto, A. |
Filiación: | Departamento de Botanica-SCB, Centro Politecnico-UFPR, CP 19031, Curitiba, PR, Brazil Departamento de Química Orgánica, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón II, (1428) Buenos Aires, Argentina
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Palabras clave: | 28-Homoethylcastasterone; Brassinosteroid derivative; Shoot proliferation; Ethanol; Fluorine compounds; Plant cell culture; Rootstocks; Plants (botany); Malus; Malus prunifolia; Malus x domestica |
Año: | 2002
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Volumen: | 20
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Número: | 11
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Página de inicio: | 1093
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Página de fin: | 1097
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DOI: |
http://dx.doi.org/10.1007/s00299-002-0442-3 |
Título revista: | Plant Cell Reports
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Título revista abreviado: | Plant Cell Rep.
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ISSN: | 07217714
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CODEN: | PCRPD
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07217714_v20_n11_p1093_Schaefer |
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Citas:
---------- APA ----------
Schaefer, S., Medeiro, S., Ramírez, J., Galagovsky, L. & Pereira-Netto, A.
(2002)
. Brassinosteroid-driven enhancement of the in vitro multiplication rate for the marubakaido apple rootstock [Malus prunifolia (Willd.) Borkh]. Plant Cell Reports, 20(11), 1093-1097.
http://dx.doi.org/10.1007/s00299-002-0442-3---------- CHICAGO ----------
Schaefer, S., Medeiro, S., Ramírez, J., Galagovsky, L., Pereira-Netto, A.
"Brassinosteroid-driven enhancement of the in vitro multiplication rate for the marubakaido apple rootstock [Malus prunifolia (Willd.) Borkh]"
. Plant Cell Reports 20, no. 11
(2002) : 1093-1097.
http://dx.doi.org/10.1007/s00299-002-0442-3---------- MLA ----------
Schaefer, S., Medeiro, S., Ramírez, J., Galagovsky, L., Pereira-Netto, A.
"Brassinosteroid-driven enhancement of the in vitro multiplication rate for the marubakaido apple rootstock [Malus prunifolia (Willd.) Borkh]"
. Plant Cell Reports, vol. 20, no. 11, 2002, pp. 1093-1097.
http://dx.doi.org/10.1007/s00299-002-0442-3---------- VANCOUVER ----------
Schaefer, S., Medeiro, S., Ramírez, J., Galagovsky, L., Pereira-Netto, A. Brassinosteroid-driven enhancement of the in vitro multiplication rate for the marubakaido apple rootstock [Malus prunifolia (Willd.) Borkh]. Plant Cell Rep. 2002;20(11):1093-1097.
http://dx.doi.org/10.1007/s00299-002-0442-3