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Abstract:

Treatment of shoot apices of the marubakaido apple rootstock with a fluoro derivative of 28-homoethylcastasterone (5F-HCTS) in the range 100-10,000 ng per shoot increased the apple rootstock's multiplication rate (MR). A dose of 500 ng 5F-HCTS in 5 μl ethanol, per shoot, resulted in a significant (P=0.05) 112% increase in MR compared to shoots treated with 5 μl of 95% ethanol when the shoots were grown in culture medium enriched with 2.2 μM N6-benzyladenine. This increase in MR was due to: (1) a 15% increase in the number of main branches; (2) a 238% increase in the number of primary lateral branches; (3) a 250% increase in the number of secondary lateral branches. These results show that shoot proliferation induced by the fluoro derivative of 28-homocastasterone is an effective method to enhance the in vitro multiplication rate in Malus prunifolia.

Registro:

Documento: Artículo
Título:Brassinosteroid-driven enhancement of the in vitro multiplication rate for the marubakaido apple rootstock [Malus prunifolia (Willd.) Borkh]
Autor:Schaefer, S.; Medeiro, S.; Ramírez, J.; Galagovsky, L.; Pereira-Netto, A.
Filiación:Departamento de Botanica-SCB, Centro Politecnico-UFPR, CP 19031, Curitiba, PR, Brazil
Departamento de Química Orgánica, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón II, (1428) Buenos Aires, Argentina
Palabras clave:28-Homoethylcastasterone; Brassinosteroid derivative; Shoot proliferation; Ethanol; Fluorine compounds; Plant cell culture; Rootstocks; Plants (botany); Malus; Malus prunifolia; Malus x domestica
Año:2002
Volumen:20
Número:11
Página de inicio:1093
Página de fin:1097
DOI: http://dx.doi.org/10.1007/s00299-002-0442-3
Título revista:Plant Cell Reports
Título revista abreviado:Plant Cell Rep.
ISSN:07217714
CODEN:PCRPD
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07217714_v20_n11_p1093_Schaefer

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Citas:

---------- APA ----------
Schaefer, S., Medeiro, S., Ramírez, J., Galagovsky, L. & Pereira-Netto, A. (2002) . Brassinosteroid-driven enhancement of the in vitro multiplication rate for the marubakaido apple rootstock [Malus prunifolia (Willd.) Borkh]. Plant Cell Reports, 20(11), 1093-1097.
http://dx.doi.org/10.1007/s00299-002-0442-3
---------- CHICAGO ----------
Schaefer, S., Medeiro, S., Ramírez, J., Galagovsky, L., Pereira-Netto, A. "Brassinosteroid-driven enhancement of the in vitro multiplication rate for the marubakaido apple rootstock [Malus prunifolia (Willd.) Borkh]" . Plant Cell Reports 20, no. 11 (2002) : 1093-1097.
http://dx.doi.org/10.1007/s00299-002-0442-3
---------- MLA ----------
Schaefer, S., Medeiro, S., Ramírez, J., Galagovsky, L., Pereira-Netto, A. "Brassinosteroid-driven enhancement of the in vitro multiplication rate for the marubakaido apple rootstock [Malus prunifolia (Willd.) Borkh]" . Plant Cell Reports, vol. 20, no. 11, 2002, pp. 1093-1097.
http://dx.doi.org/10.1007/s00299-002-0442-3
---------- VANCOUVER ----------
Schaefer, S., Medeiro, S., Ramírez, J., Galagovsky, L., Pereira-Netto, A. Brassinosteroid-driven enhancement of the in vitro multiplication rate for the marubakaido apple rootstock [Malus prunifolia (Willd.) Borkh]. Plant Cell Rep. 2002;20(11):1093-1097.
http://dx.doi.org/10.1007/s00299-002-0442-3