Artículo

Viana da Costa, T.; Yoshii-Oshiro, L.M.; López-Greco, L.S.; Melo, E.P.; Antunes de Mattos, L.; Bambozzi-Fernandes, A. "Determination of adequate voltage and animal size for the extraction of spermatophores in the freshwater prawn Macrobrachium acanthurus " (2016) Latin American Journal of Aquatic Research. 44(2):422-428
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Abstract:

So far Macrobrachium acanthurus reproduction studies are poorly advanced being important if there is the possibility of cultivation. The aim of this study was to identify the most suitable voltage for the extraction of spermatophores and the smaller size at which these prawns produce them, as a prior step to develop in vitro fertilization techniques. Voltages of 4.5 and 6.0 were used, performing sperm count based on survival of sperm smears, colored with eosin-nigrosine. In the study of size, prawns were separated into class intervals and analyzed for production of spermatophore and sperm survival. The best voltage for the extraction of seminal material due to lower mortality was 6.0 volts and from 5 g of body weight, and 18 mm of length prawns could be used for the extraction of spermatophores. © 2016, Escuela de Ciencias del Mar. All rights reserved.

Registro:

Documento: Artículo
Título:Determination of adequate voltage and animal size for the extraction of spermatophores in the freshwater prawn Macrobrachium acanthurus
Autor:Viana da Costa, T.; Yoshii-Oshiro, L.M.; López-Greco, L.S.; Melo, E.P.; Antunes de Mattos, L.; Bambozzi-Fernandes, A.
Filiación:Instituto de Ciências Sociais, Educação e Zootecnia, Universidade Federal do Amazonas, Parintins, AM 9.152-240, Brazil
Estação de Biologia Marinha, Universidade Federal Rural do Rio de Janeiro, Mangaratiba, RJ 23.860-020, Brazil
Departamento de Biodiversidad y Biología Experimental, FCEN e IBBEA, CONICET-UBA Universidad de Buenos Aires, Argentina
Palabras clave:Caridea; Macrobrachium acanthurus; Reproduction; Sperm; Sperm survival
Año:2016
Volumen:44
Número:2
Página de inicio:422
Página de fin:428
DOI: http://dx.doi.org/10.3856/vol44-issue2-fulltext-24
Título revista:Latin American Journal of Aquatic Research
Título revista abreviado:Lat. Am. J. Aquatic Res.
ISSN:0718560X
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0718560X_v44_n2_p422_VianadaCosta

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Citas:

---------- APA ----------
Viana da Costa, T., Yoshii-Oshiro, L.M., López-Greco, L.S., Melo, E.P., Antunes de Mattos, L. & Bambozzi-Fernandes, A. (2016) . Determination of adequate voltage and animal size for the extraction of spermatophores in the freshwater prawn Macrobrachium acanthurus . Latin American Journal of Aquatic Research, 44(2), 422-428.
http://dx.doi.org/10.3856/vol44-issue2-fulltext-24
---------- CHICAGO ----------
Viana da Costa, T., Yoshii-Oshiro, L.M., López-Greco, L.S., Melo, E.P., Antunes de Mattos, L., Bambozzi-Fernandes, A. "Determination of adequate voltage and animal size for the extraction of spermatophores in the freshwater prawn Macrobrachium acanthurus " . Latin American Journal of Aquatic Research 44, no. 2 (2016) : 422-428.
http://dx.doi.org/10.3856/vol44-issue2-fulltext-24
---------- MLA ----------
Viana da Costa, T., Yoshii-Oshiro, L.M., López-Greco, L.S., Melo, E.P., Antunes de Mattos, L., Bambozzi-Fernandes, A. "Determination of adequate voltage and animal size for the extraction of spermatophores in the freshwater prawn Macrobrachium acanthurus " . Latin American Journal of Aquatic Research, vol. 44, no. 2, 2016, pp. 422-428.
http://dx.doi.org/10.3856/vol44-issue2-fulltext-24
---------- VANCOUVER ----------
Viana da Costa, T., Yoshii-Oshiro, L.M., López-Greco, L.S., Melo, E.P., Antunes de Mattos, L., Bambozzi-Fernandes, A. Determination of adequate voltage and animal size for the extraction of spermatophores in the freshwater prawn Macrobrachium acanthurus . Lat. Am. J. Aquatic Res. 2016;44(2):422-428.
http://dx.doi.org/10.3856/vol44-issue2-fulltext-24