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Abstract:

The estuarine crustaceans are exposed to frequent and abrupt environmental salinity changes that involve adjustments at different levels in muscle tissue. The histochemical composition of fiber types of the claw closer muscle of Cyrtograpsus angulatus and Neohelice granulata (Dana, 1851) under hyper regulatory conditions was analyzed. Cryosections of the muscle were treated with myosin-adenosine triphosphatase (m-ATPase), succinic dehydrogenase (SDH), periodic acid Schiff (PAS) and Sudan Black B. The mean diameters, the relative area and the proportion of each muscle fiber type were calculated. Types I and IV would belong to ‘extreme’ groups, whereas types II and III would be considered ‘intermediate’. Type I fibers were large and exhibited a weak reaction to all techniques; type IV fibers were small and reacted strongly to histochemical tests. Types II and III prevailed in C. angulatus whereas type II predominated in N. granulata. Type IV fibers were absent in C. angulatus and scarce in N. granulata. The claw closer muscle of N. granulata and C. angulatus exhibited differential responses under reduced salinity at a histochemical level. Therefore, the existence of different adjustment mechanisms facing salinity stress is suggested. © 2017, Universidad de la Frontera. All rights reserved.

Registro:

Documento: Artículo
Título:Influence of reduced salinity on the histochemical fiber type composition of the claw closer muscle of two estuarine crab species, Cyrtograpsus angulatus and Neohelice granulata (Decapoda: Varunidae)
Autor:Longo, M.V.; Díaz, A.O.
Filiación:Instituto de Investigaciones Marinas y Costeras (IIMyC), FCEyN, Universidad Nacional de Mar del Plata, CONICET, Funes 3250 3° piso, Mar del Plata, Buenos Aires, Argentina
Palabras clave:Euryhaline crabs; Histochemistry; Hyper regulation; Muscle fibers; Salinity stress
Año:2017
Volumen:35
Número:2
Página de inicio:533
Página de fin:540
DOI: http://dx.doi.org/10.4067/S0717-95022017000200025
Título revista:International Journal of Morphology
Título revista abreviado:Int. J. Morphol.
ISSN:07179367
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07179367_v35_n2_p533_Longo

Referencias:

  • Alberti, J., Escapa, M., Iribarne, O., Silliman, B., Bertness, M., Crab herbivory regulates plant facilitative and competitive processes in Argentinean marshes (2008) Ecology, 89 (1), pp. 155-164
  • Bianchini, A., Lauer, M.M., Nery, L.E., Colares, E.P., Monserrat, J.M., Dos Santos Filho, E.A., Biochemical and physiological adaptations in the estuarine crab Neohelice granulata during salinity acclimation (2008) Comp. Biochem. Physiol. a Mol. Integr. Physiol, 151 (3), pp. 423-436
  • Freire, C.A., Onken, H., McNamara, J.C., A structure-function analysis of ion transport in crustacean gills and excretory organs (2008) Comp. Biochem. Physiol. a Mol. Integr. Physiol, 151 (3), pp. 272-304
  • Gruhn, M., Rathmayer, W., Phenotype plasticity in postural muscles of the crayfish Orconectes limosus Raf.: Correlation of myofibrillar ATPasebased fiber typing with electrophysiological fiber properties and the effect of chronic nerve stimulation (2002) J. Exp. Zool, 293 (2), pp. 127-140
  • Günzel, D., Galler, S., Rathmayer, W., Fibre heterogeneity in the closer and opener muscles of crayfish walking legs (1993) J. Exp. Biol, 175, pp. 267-282
  • Hardy, K.M., Dillaman, R.M., Locke, B.R., Kinsey, S., Skeletal muscle model of extreme hypertrophic growth reveals the influence of diffusion on cellular design (2009) Am. J. Physiol. Regul. Integr. Comp. Physiol, 296 (6), pp. R1855-R1867
  • Hirayama, Y., Sutoh, K., Watabe, S., Structure-function relationships of the two surface loops of myosin heavy chain isoforms from thermally acclimated carp (2000) Biochem. Biophys. Res. Commun, 269 (1), pp. 237-241
  • Hooper, S.L., Thuma, J.B., Invertebrate muscles: Muscle specific genes and proteins (2005) Physiol. Rev, 85 (3), pp. 1001-1060
  • Johnson, P.T., Histology of the Blue Crab, Callinectes Sapidus (1980) A Model for the Decapoda, , New York, Praeger Publishers
  • Kirschner, L.B., The mechanism of sodium chloride uptake in hyperregulating aquatic animals (2004) J. Exp. Biol, 207, pp. 1439-1452
  • Li, E., Chen, L., Zeng, C., Yu, N., Xiong, Z., Chen, X., Qin, J.G., Comparison of digestive and antioxidant enzymes activities, haemolymph oxyhemocyanin contents and hepatopancreas histology of white shrimp, Litopenaeus vannamei, at various salinities (2008) Aquaculture, 274 (1), pp. 80-86
  • Longo, M.V., Goldemberg, A.L., Díaz, A.O., The claw closer muscle of Neohelice granulata (Grapsoidea, Varunidae): A morphological and histochemical study (2011) Acta Zool, 92 (2), pp. 126-133
  • Longo, M.V., Díaz, A.O., The claw closer muscle of two estuarine crab species, Cyrtograpsus angulatus and Neohelice granulata (Grapsoidea, Varunidae): Histochemical fibre type composition (2013) Acta Zool, 94 (2), pp. 233-239
  • Longo, M.V., Díaz, A.O., Morphology of the claw closer muscle in two estuarine crab species (Crustacea, Varunidae): An ultrastructural study (2013) Zoolog. Sci, 30 (8), pp. 663-669
  • Longo, M.V., Díaz, A.O., Histological and histochemical study of the hepatopancreas of two estuarine crab species, Cyrtograpsus angulatus and Neohelice granulata (Grapsoidea, Varunidae) (2015) Influence of Environmental Salinity. Zool. Sci, 32 (2), pp. 163-170
  • López-Albors, O., Ayala, M.D., Gil, F., García-Alcázar, A., Abellán, E., Latorre, R., Ramírez-Zarzosa, G., Vázquez, J.M., Early temperature effects on muscle growth dynamics and histochemical profile of muscle fibres of sea bass Dicentrarchus labrax L., during larval and juvenile stages (2003) Aquaculture, 220 (1), pp. 385-406
  • Luvizotto-Santos, R., Lee, J.T., Branco, Z.P., Bianchini, A., Nery, L.E., Lipids as energy source during salinity acclimation in the euryhaline crab Chasmagnathus granulata dana, 1851 (Crustacea-grapsidae) (2003) J. Exp. Zool. a Comp. Exp. Biol, 295 (2), pp. 200-205
  • Maier, L., Rathmayer, W., Pette, D., PH lability of myosin ATPase activity permits discrimination of different muscle fibre types in crustaceans (1984) Histochemistry, 81 (1), pp. 75-77
  • Martins, T.L., Chittó, A.L., Rossetti, C.L., Brondani, C.K., Kucharski, L.C., Da, S., R. S. Effects of hypo-or hyperosmotic stress on lipid synthesis and gluconeogenic activity in tissues of the crab Neohelice granulata (2011) Comp. Biochem. Physiol. a Mol. Integr. Physiol, 158 (4), pp. 400-405
  • McManus, J.F., Histological and histochemical uses of periodic acid (1948) Stain Technol, 23 (3), pp. 99-108
  • McNamara, J.C., Faria, S.C., Evolution of osmoregulatory patterns and gill ion transport mechanisms in the decapod Crustacea: A review (2012) J. Comp. Physiol. B, 182 (8), pp. 997-1014
  • Michiels, M.S., Del Valle, J.C., López Mananes, A.A., Effect of environmental salinity and dopamine injections on key digestive enzymes in hepatopancreas of the euryhaline crab Cyrtograpsus angulatus (Decapoda: Brachyura: Varunidae (2013) Sci. Mar, 77 (1), pp. 129-136
  • Moriyasu, M., Mallet, P., Molt Stages of the Snow Crab Chionoecetes opilio by Observation of Morphogenesis of Setae on the Maxilla (1986) J. Crustacean Biol, 6 (4), pp. 709-718
  • Neil, D.M., Fowler, W.S., Tobasnick, G., Myofibrillar protein composition correlates with histochemistry in fibres of the abdominal flexor muscles of the Norway lobster Nephrops norvegicus (1993) J. Exp. Biol, 183, pp. 185-202
  • Nery, L.E.M., Santos, E.A., Carbohydrate metabolism during osmoregulation in Chasmagnathus granulata Dana, 1851 (Crustacea-Decapoda) (1993) Comp. Biochem. Physiol. B, 106, pp. 747-753
  • Olivier, S.R., Escofet, A., Penchaszadeh, P.E., Oresanz, J.M., Estudios ecológicos en la región estuarial de Mar Chiquita (Buenos Aires, Argentina). II. Relaciones tróficas interespecíficas (1972) An. Com. Investig. Cient, 1972, pp. 89-104
  • Perry, M.J., Tait, J., Hu, J., White, S.C., Medler, S., Skeletal muscle fiber types in the ghost crab, Ocypode quadrata: Implications for running performance (2009) J. Exp. Biol, 212, pp. 673-683
  • Pinoni, S.A., López Mañanes, A.A., Partial characterization and response under hyperregulating conditions of Na+-K+ ATPase and levamisolesensitive alkaline phosphatase activities in chela muscle of the euryhaline crab Cyrtograpsus angulatus. Sci (2008) Mar, 72 (1), pp. 15-24
  • Pinoni, S.A., López Mañanes, A.A., Na+ ATPase activities in chela muscle of the euryhaline crab Neohelice granulata: Differential response to environmental salinity (2009) J. Exp. Mar. Biol. Ecol, 372 (1), pp. 91-97
  • Pinoni, S.A., Michiels, M.S., López Mañanes, A.A., Phenotypic flexibility in response to environmental salinity in the euryhaline crab Neohelice granulata from the mudflat and the saltmarsh of a SW coastal lagoon (2013) Mar. Biol, 160, pp. 2647-2661
  • Rock, J., Magnay, J.L., Beech, S., El Haj, A.J., Goldspink, G., Lunt, D.H., Whiteley, N.M., Linking functional molecular variation with environmental gradients: Myosin gene diversity in a crustacean broadly distributed across variable thermal environments (2009) Gene, 437 (1-2), pp. 60-70
  • Romano, N., Zeng, C., Osmoregulation in decapod crustaceans: Implications to aquaculture productivity, methods for potential improvement and interactions with elevated ammonia exposure (2012) Aquaculture, , 334-337:12-23
  • Schein, V., Chittó, A.L.F., Etges, R., Kucharski, L.C., Van Wormhoudt, A., Da Silva, R.S.M., Effects of hypo-or hyperosmotic stress on gluconeogenesis, phosphoenolpyruvate carboxykinase activity, and gene expression in jaw muscle of the crab Chasmagnathus granulata: Seasonal differences (2005) J. Exp. Mar. Biol. Ecol, 316 (2), pp. 203-212
  • Spivak, E.D., Cangrejos estuariales del Atlántico sudoccidental (25°-41°S) (Crustacea: Decapoda: Brachyura) (1997) Invest. Mar, 25, pp. 105-120
  • Vinagre, A.S., Nunes Do Amaral, A.P., Ribarcki, F.P., Fraga Da Silveira, E., Périco, E., Seasonal variation of energy metabolism in ghost crab Ocypode quadrata at Siriú Beach (Brazil) (2007) Comp. Biochem. Physiol. a Mol. Integr. Physiol, 146 (4), pp. 514-519
  • Zar, J.H., (2010) Biostatistical Analysis, , 5thed. New Jersey, Pearson Prentice Hall

Citas:

---------- APA ----------
Longo, M.V. & Díaz, A.O. (2017) . Influence of reduced salinity on the histochemical fiber type composition of the claw closer muscle of two estuarine crab species, Cyrtograpsus angulatus and Neohelice granulata (Decapoda: Varunidae) . International Journal of Morphology, 35(2), 533-540.
http://dx.doi.org/10.4067/S0717-95022017000200025
---------- CHICAGO ----------
Longo, M.V., Díaz, A.O. "Influence of reduced salinity on the histochemical fiber type composition of the claw closer muscle of two estuarine crab species, Cyrtograpsus angulatus and Neohelice granulata (Decapoda: Varunidae) " . International Journal of Morphology 35, no. 2 (2017) : 533-540.
http://dx.doi.org/10.4067/S0717-95022017000200025
---------- MLA ----------
Longo, M.V., Díaz, A.O. "Influence of reduced salinity on the histochemical fiber type composition of the claw closer muscle of two estuarine crab species, Cyrtograpsus angulatus and Neohelice granulata (Decapoda: Varunidae) " . International Journal of Morphology, vol. 35, no. 2, 2017, pp. 533-540.
http://dx.doi.org/10.4067/S0717-95022017000200025
---------- VANCOUVER ----------
Longo, M.V., Díaz, A.O. Influence of reduced salinity on the histochemical fiber type composition of the claw closer muscle of two estuarine crab species, Cyrtograpsus angulatus and Neohelice granulata (Decapoda: Varunidae) . Int. J. Morphol. 2017;35(2):533-540.
http://dx.doi.org/10.4067/S0717-95022017000200025