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Abstract:

We tested the effects of the flavonoid 3-methoxi-5,6,7,8-hydroxy-4'hydroxy flavone (NMHTV) isolated from shoots of non arbuscular mycorrhizal (AM) inoculated clover, and of the flavonoids 5,6,7,8-hydroxy-3-methoxy flavone (MH-1); 5,6,7,8-hydroxy-4'- hydroxy flavone (MH-2); and 5,7-hydroxy-3,4'-methoxy flavone (MH-3); isolated from AM clover (Trifolium repens) shoots, on spore germination, hyphal length, hyphal branches and the number of cluster of auxiliary cells or the number of secondary spores (Presymbiotic stage) and on the number of entry points and the percentage of AM colonized root of tomato (Lycopersicum esculentum) by the AM fungi Gigaspora rosea, Giaspora margarita, Glomus mosseae and Glomus intraradices (Symbiotic stage). Non significant effects of the flavonoids isolated from the shoot of mycorrhizal colonized clover on the presymbiotic and symbiotic stages of Gigaspora and Glomus endophytes were found. The flavonoid NMHTV isolated from non AM clover shoot, did not affect the percentage of germination of spores but significantly increased (P < 0.05) the other steps of the presymbiotic stage of Gi. margarita spores when 2 μM concentration was used. The symbiotic stage of Gi. margarita was also significantly increased when 2 μM of the flavonoid NMHTV was applied. This flavonoid had no effect on the presymbiotic development of G. mosseae, G. intraradices and Gi. rosea except when 8 μM concentration was used, which inhibited the hyphal length of Gi. rosea. These results suggest the possible implication of the flavonoid NMHTV in the susceptibility of tomato roots to the AM formation by Gi. margarita. The absence of stimulation of the AM presymbiotic and symbiotic stages in tomato by exogenous application of the newly synthesized flavonoids MH-1, MH-2, and MH-3, in clover shoots after AM colonization, indicated that the autorregulation of the AM symbiosis can be, at least partially, due to the disappearance of flavonoids in AM colonized plants that stimulated the AM symbiosis.

Registro:

Documento: Artículo
Título:Development of arbuscular mycorrhizal fungi in the presence of different patterns of Trifolium repens shoot flavonoids
Autor:Scervino, J.M.; Ponce, M.A.; Monica, I.D.; Vierheilig, H.; Ocampo, J.A.; Godeas, A.
Filiación:Departamento de Biodiversidad y Biología Experimental, Universidad de Buenos Aires, Pabellón II, Buenos Aires, Argentina
CIHIDECAR-CONICET, Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón II, Buenos Aires, Argentina
Institut für Pflanzenschutz, Department für Angewandte Pflanzenwissenschaften und Pflanzenbiotechnologie, Universität für Bodenkultur Wien, Peter-Jordan-Strasse 82, 1190 Wien, Austria
Departamento de Microbiología, Estación Experimental de Zaidín, CSIC, Prof. Albareda 1, E-18008 Granada, Spain
Palabras clave:Arbuscular mycorrhizas; Flavonoids; Signal molecules; Symbiosis; Arbuscular; Bacteria (microorganisms); Fungi; Gigaspora; Gigaspora rosea; Glomus; Glomus intraradices; Glomus mosseae; Lycopersicon esculentum; Trifolium; Trifolium repens
Año:2009
Volumen:9
Número:2
Página de inicio:102
Página de fin:115
DOI: http://dx.doi.org/10.4067/S0718-27912009000200002
Título revista:Revista de la Ciencia del Suelo y Nutricion Vegetal
Título revista abreviado:Rev. Cienc. Suelo Nutr. Vegetal
ISSN:0717635X
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_0717635X_v9_n2_p102_Scervino.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0717635X_v9_n2_p102_Scervino

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Citas:

---------- APA ----------
Scervino, J.M., Ponce, M.A., Monica, I.D., Vierheilig, H., Ocampo, J.A. & Godeas, A. (2009) . Development of arbuscular mycorrhizal fungi in the presence of different patterns of Trifolium repens shoot flavonoids. Revista de la Ciencia del Suelo y Nutricion Vegetal, 9(2), 102-115.
http://dx.doi.org/10.4067/S0718-27912009000200002
---------- CHICAGO ----------
Scervino, J.M., Ponce, M.A., Monica, I.D., Vierheilig, H., Ocampo, J.A., Godeas, A. "Development of arbuscular mycorrhizal fungi in the presence of different patterns of Trifolium repens shoot flavonoids" . Revista de la Ciencia del Suelo y Nutricion Vegetal 9, no. 2 (2009) : 102-115.
http://dx.doi.org/10.4067/S0718-27912009000200002
---------- MLA ----------
Scervino, J.M., Ponce, M.A., Monica, I.D., Vierheilig, H., Ocampo, J.A., Godeas, A. "Development of arbuscular mycorrhizal fungi in the presence of different patterns of Trifolium repens shoot flavonoids" . Revista de la Ciencia del Suelo y Nutricion Vegetal, vol. 9, no. 2, 2009, pp. 102-115.
http://dx.doi.org/10.4067/S0718-27912009000200002
---------- VANCOUVER ----------
Scervino, J.M., Ponce, M.A., Monica, I.D., Vierheilig, H., Ocampo, J.A., Godeas, A. Development of arbuscular mycorrhizal fungi in the presence of different patterns of Trifolium repens shoot flavonoids. Rev. Cienc. Suelo Nutr. Vegetal. 2009;9(2):102-115.
http://dx.doi.org/10.4067/S0718-27912009000200002