Abstract:
We tested the effects of the flavonoid 3-methoxi-5,6,7,8-hydroxy-4'hydroxy flavone (NMHTV) isolated from shoots of non arbuscular mycorrhizal (AM) inoculated clover, and of the flavonoids 5,6,7,8-hydroxy-3-methoxy flavone (MH-1); 5,6,7,8-hydroxy-4'- hydroxy flavone (MH-2); and 5,7-hydroxy-3,4'-methoxy flavone (MH-3); isolated from AM clover (Trifolium repens) shoots, on spore germination, hyphal length, hyphal branches and the number of cluster of auxiliary cells or the number of secondary spores (Presymbiotic stage) and on the number of entry points and the percentage of AM colonized root of tomato (Lycopersicum esculentum) by the AM fungi Gigaspora rosea, Giaspora margarita, Glomus mosseae and Glomus intraradices (Symbiotic stage). Non significant effects of the flavonoids isolated from the shoot of mycorrhizal colonized clover on the presymbiotic and symbiotic stages of Gigaspora and Glomus endophytes were found. The flavonoid NMHTV isolated from non AM clover shoot, did not affect the percentage of germination of spores but significantly increased (P < 0.05) the other steps of the presymbiotic stage of Gi. margarita spores when 2 μM concentration was used. The symbiotic stage of Gi. margarita was also significantly increased when 2 μM of the flavonoid NMHTV was applied. This flavonoid had no effect on the presymbiotic development of G. mosseae, G. intraradices and Gi. rosea except when 8 μM concentration was used, which inhibited the hyphal length of Gi. rosea. These results suggest the possible implication of the flavonoid NMHTV in the susceptibility of tomato roots to the AM formation by Gi. margarita. The absence of stimulation of the AM presymbiotic and symbiotic stages in tomato by exogenous application of the newly synthesized flavonoids MH-1, MH-2, and MH-3, in clover shoots after AM colonization, indicated that the autorregulation of the AM symbiosis can be, at least partially, due to the disappearance of flavonoids in AM colonized plants that stimulated the AM symbiosis.
Registro:
Documento: |
Artículo
|
Título: | Development of arbuscular mycorrhizal fungi in the presence of different patterns of Trifolium repens shoot flavonoids |
Autor: | Scervino, J.M.; Ponce, M.A.; Monica, I.D.; Vierheilig, H.; Ocampo, J.A.; Godeas, A. |
Filiación: | Departamento de Biodiversidad y Biología Experimental, Universidad de Buenos Aires, Pabellón II, Buenos Aires, Argentina CIHIDECAR-CONICET, Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón II, Buenos Aires, Argentina Institut für Pflanzenschutz, Department für Angewandte Pflanzenwissenschaften und Pflanzenbiotechnologie, Universität für Bodenkultur Wien, Peter-Jordan-Strasse 82, 1190 Wien, Austria Departamento de Microbiología, Estación Experimental de Zaidín, CSIC, Prof. Albareda 1, E-18008 Granada, Spain
|
Palabras clave: | Arbuscular mycorrhizas; Flavonoids; Signal molecules; Symbiosis; Arbuscular; Bacteria (microorganisms); Fungi; Gigaspora; Gigaspora rosea; Glomus; Glomus intraradices; Glomus mosseae; Lycopersicon esculentum; Trifolium; Trifolium repens |
Año: | 2009
|
Volumen: | 9
|
Número: | 2
|
Página de inicio: | 102
|
Página de fin: | 115
|
DOI: |
http://dx.doi.org/10.4067/S0718-27912009000200002 |
Título revista: | Revista de la Ciencia del Suelo y Nutricion Vegetal
|
Título revista abreviado: | Rev. Cienc. Suelo Nutr. Vegetal
|
ISSN: | 0717635X
|
PDF: | https://bibliotecadigital.exactas.uba.ar/download/paper/paper_0717635X_v9_n2_p102_Scervino.pdf |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0717635X_v9_n2_p102_Scervino |
Referencias:
- Akiyama, K., Matsuoka, H., Hayashi, H., Isolation and identification of a phosphate deficiency-induced C-glycosylflavonoid that stimulates arbuscular mycorrhiza formation in melon roots (2002) Molecular Plant Microbe Interaction, 15, pp. 334-340
- Baptista, M.J., Siqueira, J.O., Efeito de flavonóides na germinação e no crescimento assimbiótico de fungos micorrízicos vesículoarbusculares (1994) Revista Brasileira de Fisiologia Vegetal, 6, pp. 127-134
- Bécard, G., Douds, D.D., Pfeffer, P.E., Extensive in vitro hyphal growth of vesiculararbuscular mycorrhizal fungi in the presence of CO2 and flavonols (1992) Applied and Environmental Microbiology, 58, pp. 821-825
- Bécard, G., Piche, Y., Fungal growth stimulation by CO2 and root exudates in vesicular-arbuscular mycorrhizal symbiosis (1989) Applied and Environmental Microbiology, 55, pp. 2320-2325
- Bentivenga, S.P., Morton, J.B., A monograph of the genus Gigaspora, incorporating developmental patterns of morphological characters (1995) Mycologia, 87, pp. 720-732
- Buee, M., Rossignol, M., Jauneau, A., Ranjeva, R., Becard, G., The pre-symbiotic growth of arbuscular mycorrhizal fungi is induced by a branching factor partially purified from plant root exudates (2000) Molecular Plant Microbe Interaction, 13, pp. 693-698
- Chabot, S., Bel-Rhlid, T., Chenevert, R., Piche, Y., Hyphae growth promotion in vitro of the VA mycorrhizal fungus, Gigaspora margarita Becker and Hall, by the activity of structurally specific flavonoid compounds under CO2 enriched conditions (1992) The New Phytologist., 122, pp. 461-467
- Fracchia, S., (2002) Hongos saprotrofos del suelo como microorganismos auxiliares de la micorrización, , Ph.D. Thesis, Universidad de Buenos Aires, Argentina
- Fracchia, S., Menendez, A., Godeas, A., Ocampo, J.A., A method to obtain monosporic cultures of arbuscular mycorrhizal fungi (2001) Soil Biology and Biochemistry, 33, pp. 1283-1285
- Gerdemann, J.W., Relation of a large soilborne spore to phycomycetous mycorrhizal infections (1955) Mycologia, 47, pp. 619-632
- Giovannetti, M., Mosse, B., An evaluation of techniques for measuring vesicular-arbuscular mycorrhizal infection in roots (1980) The New Phytologist, 84, pp. 489-500
- Giovannetti, M., Sbrana, C., Citernesi, A.S., Avio, L., Analysis of factor involved in fungal recognition responses to host-derived signals by arbuscular mycorrhizal fungi (1996) The New Phytologist, 133, pp. 65-71
- Harrison, M.J., Development of the arbuscular mycorrhizal symbiosis (1998) Current Opinion Plant Biology, 1, pp. 360-365
- Kape, R., Wex, K., Parniske, M., Görge, E., Wetzel, A., Werner, D., Legume root metabolites and VA-mycorrhiza development (1992) Journal Plant Physiologist, 141, pp. 54-60
- Larose, G., Chenevert, R., Moutoglis, P., Gagne, S., Piche, Y., Vierheilig, H., Flavonoid levels in roots of Medicago sativa are modulated by the developmental stage of the symbiosis and the root colonizing arbuscular mycorrhizal fungus (2002) Journal of Plant Physiology, 159, pp. 1329-1339
- Marsh, B.A.B., Measurement of length in random arrangements of lines (1971) Journal of Applied Ecology, 8, pp. 265-270
- Martin, J., Sampedro, I., Garcia-Romera, I., Garcia-Garrido, J.M., Ocampo, J.A., Arbuscular mycorrhizal colonization and growth of soybean (Glycine max) and lettuce (Lactuca sativa) and phytotoxic effects of olive mill residues (2002) Soil Biology and Biochemistry, 34, pp. 1769-1775
- Morandi, D., Occurrence of phytoalexins and phenolic compounds in endomycorrhizal interactions, and their potential role in biological control (1996) Plant and Soil, 185, pp. 241-251
- Morandi, D., Branzanti, B., Gianinazzi-Pearson, V., Effect of Some Plant Flavonoid on in Vitro Behavior of an Arbuscular Mycorrhizal Fungus (1992) Agronomie, 12, pp. 811-816
- Mosse, B., The establishment of vesicular arbuscular mycorrhiza under aseptic conditions (1962) Journal General Microbiology, 27, pp. 509-520
- Ocampo, J.A., Martin, J., Hayman, D.S., Influence of Plant Interactions on Vesicular-Arbuscular Mycorrhizal Infections.1. Host and Non-Host Plants Grown Together (1980) New Phytologist., 84, pp. 27-35
- Paula, M.A., Siqueira, J.O., Stimulation of hyphal growthof the VA mycorrhizal fungus Gigaspora margarita by suspension-cultured Pueraria phaseoloides (1990) New Phytologist, 115, pp. 69-75
- Phillips, D.A., Tsai, S.M., Flavonoids as plant signals to the rhizosphere microbes (1992) Mycorrhiza, 1, pp. 55-58
- Phillips, J.M., Hayman, D.S., Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection (1970) Transactions of the British Mycological Society, 55, pp. 158-161
- Ponce, M., Cervino, M., Erra-Balsells, R., Ocampo, J.A., Godeas, A., Flavonoids from shoots and roots of Trifolium repens (white clover) grown in presence or absence of the arbuscular mycorrhizal fungus Glomus intraradices (2004) Phytochemistry, 65, pp. 3131-3134
- Poulin, M.J., Simard, J., Catford, J.G., Labrie, F., Piche, Y., Response of symbiotic endomycorrhizal fungi to estrogens and antiestrogens (1997) Molecular Plant Microbe Interaction, 10, pp. 481-487
- Poulton, J.L., Koide, R.T., Stephenson, A.G., Effects of mycorrhizal infection and high soil phosphorous improve vegetative growth and the female and male functions in tomato (2002) The New Phytologist, 154, pp. 255-264
- Siqueira, J.O., Safir, G.R., Nair, M.G., Stimulation of vesicular-arbuscular mycorrhiza Formation and growth of white clover by flavonoid compounds (1991) The New Phytologist, 118, pp. 87-93
- Taylor, J., Harrier, L.A., Expression studies of plant genes differentially expressed in leaf and root tissues of tomato colonised by the arbuscular mycorrhizal fungus Glomus mosseae (2003) Plant Molecular Biology, 51, pp. 619-629
- Tsai, S.M., Phillips, D.A., Flavonoids released naturally from alfalfa promote development of symbiotic Glomus spores in vitro (1991) Applied and Environmental Microbiology, 57, pp. 1485-1488
- Vierheilig, H., Bago, B., Albrecht, C., Poulin, M.J., Piche, Y., Flavonoids and arbuscular mycorrhizal fungi (1998) Flavonoids in the Living System, 439, pp. 9-33
- Vierheilig, H., Bago, B., Lerat, S., Piché, Y., Shoot-produced, light-dependent factors are partially involved in the expression of the arbuscular mycorrhizal (AM) status of AM host and non-host plants (2002) Journal Plant Nutrition Soil Science, 165, pp. 21-25
- Vierheilig, H., Lerat, S., Piche, Y., Systemic inhibition of arbuscular mycorrhiza development by root exudates of cucumber plants colonized by Glomus mosseae (2003) Mycorrhiza, 13, pp. 167-170
- Vierheilig, H., Piche, Y., Signalling in arbuscular mycorrhiza: Facts and hypotheses (2002) Flavonoids in Cell Function, 505, pp. 23-39
Citas:
---------- APA ----------
Scervino, J.M., Ponce, M.A., Monica, I.D., Vierheilig, H., Ocampo, J.A. & Godeas, A.
(2009)
. Development of arbuscular mycorrhizal fungi in the presence of different patterns of Trifolium repens shoot flavonoids. Revista de la Ciencia del Suelo y Nutricion Vegetal, 9(2), 102-115.
http://dx.doi.org/10.4067/S0718-27912009000200002---------- CHICAGO ----------
Scervino, J.M., Ponce, M.A., Monica, I.D., Vierheilig, H., Ocampo, J.A., Godeas, A.
"Development of arbuscular mycorrhizal fungi in the presence of different patterns of Trifolium repens shoot flavonoids"
. Revista de la Ciencia del Suelo y Nutricion Vegetal 9, no. 2
(2009) : 102-115.
http://dx.doi.org/10.4067/S0718-27912009000200002---------- MLA ----------
Scervino, J.M., Ponce, M.A., Monica, I.D., Vierheilig, H., Ocampo, J.A., Godeas, A.
"Development of arbuscular mycorrhizal fungi in the presence of different patterns of Trifolium repens shoot flavonoids"
. Revista de la Ciencia del Suelo y Nutricion Vegetal, vol. 9, no. 2, 2009, pp. 102-115.
http://dx.doi.org/10.4067/S0718-27912009000200002---------- VANCOUVER ----------
Scervino, J.M., Ponce, M.A., Monica, I.D., Vierheilig, H., Ocampo, J.A., Godeas, A. Development of arbuscular mycorrhizal fungi in the presence of different patterns of Trifolium repens shoot flavonoids. Rev. Cienc. Suelo Nutr. Vegetal. 2009;9(2):102-115.
http://dx.doi.org/10.4067/S0718-27912009000200002