Artículo
Fiszman, G.L.; Karara, A.L.; Finocchiaro, L.M.E.; Glikin, G.C. "A laboratory scale device for microencapsulation of genetically engineered cells into alginate beads" (2002) Electronic Journal of Biotechnology. 5(3):86-90
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Abstract:
The microencapsulation of recombinant cells, widely used for in vitro high-density cell culture, is a novel and potentially cost-effective method of in vivo heterologous protein delivery, where the protein producing cells are immunologically protected from tissue rejection. We report here a simple, reliable and inexpensive laboratory method to generate calcium alginate microcapsules containing genetically engineered, interleukin-2 expressing, Chinese hamster ovary (CHO) cells.
Registro:
| Documento: | Artículo |
| Título: | A laboratory scale device for microencapsulation of genetically engineered cells into alginate beads |
| Autor: | Fiszman, G.L.; Karara, A.L.; Finocchiaro, L.M.E.; Glikin, G.C. |
| Filiación: | Unidad de Transferencia Genetica, Inst. de Oncologia A.H. Roffo - UBA, Av. San Martín 5481, 1417 Buenos Aires, Argentina INGEBI-CONICET/FCEN-UBA, Argentina |
| Palabras clave: | CHO cells; Interleukin-2; Protein delivery; calcium alginate; interleukin 2; animal cell; article; CHO cell; generator; microcapsule; microencapsulation; nonhuman; technique; Animalia; Cricetinae; Cricetulus griseus |
| Año: | 2002 |
| Volumen: | 5 |
| Número: | 3 |
| Página de inicio: | 86 |
| Página de fin: | 90 |
| DOI: | http://dx.doi.org/10.2225/vol5-issue3-fulltext-5 |
| Título revista: | Electronic Journal of Biotechnology |
| Título revista abreviado: | Electron. J. Biotechnol. |
| ISSN: | 07173458 |
| CAS: | calcium alginate, 9005-35-0; interleukin 2, 85898-30-2 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07173458_v5_n3_p86_Fiszman |
Referencias:
- Chang, P.L., Van Raamsdonk, J.M., Hortelano, G., Barsoum, S.C., McDonald, N.C., Stockley, T.L., The in vivo delivery of heterologous proteins by microencapsulated recombinant cells (1999) Trends in Biotechnology, 17, pp. 78-83. , February
- Ross, C.J.D., Ralph, M., Chang, P.L., Delivery of Recombinant gene products to the central nervous system with non autologous cells in alginate microcapsules (1999) Human Gene Therapy, 10, pp. 49-59
- Sun, A.M., Microencapsulation of pancreatic islet cells: A bioartificial endocrine pancreas (1988) Methods in Enzymology, 137, pp. 575-580
Citas:
---------- APA ----------
Fiszman, G.L., Karara, A.L., Finocchiaro, L.M.E. & Glikin, G.C. (2002) . A laboratory scale device for microencapsulation of genetically engineered cells into alginate beads. Electronic Journal of Biotechnology, 5(3), 86-90.http://dx.doi.org/10.2225/vol5-issue3-fulltext-5
---------- CHICAGO ----------
Fiszman, G.L., Karara, A.L., Finocchiaro, L.M.E., Glikin, G.C. "A laboratory scale device for microencapsulation of genetically engineered cells into alginate beads" . Electronic Journal of Biotechnology 5, no. 3 (2002) : 86-90.http://dx.doi.org/10.2225/vol5-issue3-fulltext-5
---------- MLA ----------
Fiszman, G.L., Karara, A.L., Finocchiaro, L.M.E., Glikin, G.C. "A laboratory scale device for microencapsulation of genetically engineered cells into alginate beads" . Electronic Journal of Biotechnology, vol. 5, no. 3, 2002, pp. 86-90.http://dx.doi.org/10.2225/vol5-issue3-fulltext-5
---------- VANCOUVER ----------
Fiszman, G.L., Karara, A.L., Finocchiaro, L.M.E., Glikin, G.C. A laboratory scale device for microencapsulation of genetically engineered cells into alginate beads. Electron. J. Biotechnol. 2002;5(3):86-90.http://dx.doi.org/10.2225/vol5-issue3-fulltext-5
