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Abstract:

In order to saturate a sunflower genetic map and facilitate marker-assisted selection (MAS) breeding for stress response, it is necessary to enhance map saturation with molecular markers localized in linkage groups associated to genomic regions involved in these traits. This work describes the identification and characterization of 1,134 simple sequence repeat (SSR) containing expressed sequence tags (ESTs) from unigenes available databases. Twelve of these functional markers as well as 41 public SSR markers were successfully localized in linkage groups, thus contributing to the saturation of specific regions on a reference genetic-linkage-map derived from recombinant inbred lines (RIL) mapping population from the cross between PAC2 x RHA266 lines. The enriched map includes 547 markers (231 SSR, 9 EST-SSR, 3 insertions/deletions (InDels) and 304 amplified fragment length polymorphisms (AFLPs) distributed in 17 linkage groups (LG), spanning genetic size to 1,942.3 cM and improving its mean density to 3.6 cM per locus. As consequence, no gaps longer than 13.2 cM remain uncovered throughout the entire map, which increases the feasibility of detecting genes or traits of agronomic importance in sunflower. © 2010 by Pontificia Universidad Católica de Valparaíso, Chile.

Registro:

Documento: Artículo
Título:Genetic mapping of EST-SSRs, SSR and InDels to improve saturation of genomic regions in a previously developed sunflower map
Autor:Talia, P.; Nishinakamasu, V.; Hopp, H.E.; Heinz, R.A.; Paniego, N.
Filiación:Instituto de Biotecnología, CICVyA, CNIA, INTA Castelar, Buenos Aires, Argentina
Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:EST-SSR; InDels; Linkage map; SSR; Sunflower; EST-SSR; InDels; Linkage map; SSR; Sunflower; Plants (botany); Genes; agronomic trait; amplified fragment length polymorphism; article; controlled study; expressed sequence tag; gene deletion; gene insertion; gene locus; gene mapping; genetic database; genetic linkage; genetic recombination; genetic trait; inbred strain; linkage analysis; nonhuman; nucleotide sequence; plant gene; polymerase chain reaction; simple sequence repeat; sunflower; Helianthus
Año:2010
Volumen:13
Número:6
DOI: http://dx.doi.org/10.2225/vol13-issue6-fulltext-14
Título revista:Electronic Journal of Biotechnology
Título revista abreviado:Electron. J. Biotechnol.
ISSN:07173458
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07173458_v13_n6_p_Talia

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  • Talia, P., Nishinakamasu, V., Hopp, H.E., Heinz, R.A., Paniego, N., Genetic mapping of EST-SSRs, SSR and InDels to improve saturation of genomic regions in a previously developed sunflower map (2010) Electronic Journal of Biotechnology, 13 (6). , http://dx.doi.org/10.2225/vol13-issue6-fulltext-14

Citas:

---------- APA ----------
Talia, P., Nishinakamasu, V., Hopp, H.E., Heinz, R.A. & Paniego, N. (2010) . Genetic mapping of EST-SSRs, SSR and InDels to improve saturation of genomic regions in a previously developed sunflower map. Electronic Journal of Biotechnology, 13(6).
http://dx.doi.org/10.2225/vol13-issue6-fulltext-14
---------- CHICAGO ----------
Talia, P., Nishinakamasu, V., Hopp, H.E., Heinz, R.A., Paniego, N. "Genetic mapping of EST-SSRs, SSR and InDels to improve saturation of genomic regions in a previously developed sunflower map" . Electronic Journal of Biotechnology 13, no. 6 (2010).
http://dx.doi.org/10.2225/vol13-issue6-fulltext-14
---------- MLA ----------
Talia, P., Nishinakamasu, V., Hopp, H.E., Heinz, R.A., Paniego, N. "Genetic mapping of EST-SSRs, SSR and InDels to improve saturation of genomic regions in a previously developed sunflower map" . Electronic Journal of Biotechnology, vol. 13, no. 6, 2010.
http://dx.doi.org/10.2225/vol13-issue6-fulltext-14
---------- VANCOUVER ----------
Talia, P., Nishinakamasu, V., Hopp, H.E., Heinz, R.A., Paniego, N. Genetic mapping of EST-SSRs, SSR and InDels to improve saturation of genomic regions in a previously developed sunflower map. Electron. J. Biotechnol. 2010;13(6).
http://dx.doi.org/10.2225/vol13-issue6-fulltext-14