Artículo
Abstract:
A peptide from hindguts of the Triatoma infestans, the hematophagous Chagus' insect vector, activates adenylyl cyclase activity in Trypanosoma cruzi epimastigote membranes and stimulates the in vitro differentiation of epimastigotes (proliferative and non-infectious forms) to metacyclic trypomastigotes (non-proliferative and infectious forms). The peptide was purified from hindguts of insects fed two days before with chicken blood. After purification, the peptide showed upon SDS-PAGE a single band of about 10 kDa. The sequence for 20 residues of the amino terminus of this peptide was: H2N-Met-Leu-Thr-Ala-Glu-Asp-Lys-Lys-Leu-Ile-Gln-Gln-Ala-Trp-Glu-Lys-A la-Ala-Ser-His. This sequence corresponds to the amino terminus of chicken α(D)-globin. A synthetic peptide with the sequence of the 40 amino acids corresponding to the amino terminus of α(D)-globin, also stimulated T. cruzi adenylyl cyclase activity and promoted metacyclogenesis.
Registro:
| Documento: | Artículo |
| Título: | An α(D)-globin fragment from Triatoma infestans hindgut stimulates Trypanosoma cruzi adenylyl cyclase and promotes metacyclogenesis |
| Autor: | Fraidenraich, D.; Pena, C.; Isola, E.L.; Lammel, E.M.; Coso, O.; Diaz Anel, A.; Baralle, F.; Torres, H.N.; Flawia, M.M. |
| Filiación: | Instituto de Investigaciones en, Ingenieria Genetica/Biol. Molecular, Obligado 2490, 1428 Buenos Aires, Argentina |
| Palabras clave: | adenylate cyclase; alpha globin; peptide; animal tissue; conference paper; enzyme activation; epimastigote; insect; intestine; nonhuman; trypanosoma cruzi; Adenylate Cyclase; Amino Acid Sequence; Animal; Chickens; Digestive Physiology; Globins; Mice; Molecular Sequence Data; Peptide Fragments; Support, Non-U.S. Gov't; Triatoma; Trypanosoma cruzi |
| Año: | 1993 |
| Volumen: | 26 |
| Número: | 1-2 |
| Página de inicio: | 279 |
| Página de fin: | 283 |
| Título revista: | Biological Research |
| Título revista abreviado: | BIOL. RES. |
| ISSN: | 07169760 |
| CODEN: | BESEE |
| CAS: | adenylate cyclase, 9012-42-4; Adenylate Cyclase, EC 4.6.1.1; Globins, 9004-22-2; Peptide Fragments |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07169760_v26_n1-2_p279_Fraidenraich |
Citas:
---------- APA ----------
Fraidenraich, D., Pena, C., Isola, E.L., Lammel, E.M., Coso, O., Diaz Anel, A., Baralle, F.,..., Flawia, M.M. (1993) . An α(D)-globin fragment from Triatoma infestans hindgut stimulates Trypanosoma cruzi adenylyl cyclase and promotes metacyclogenesis. Biological Research, 26(1-2), 279-283.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07169760_v26_n1-2_p279_Fraidenraich [ ]
---------- CHICAGO ----------
Fraidenraich, D., Pena, C., Isola, E.L., Lammel, E.M., Coso, O., Diaz Anel, A., et al. "An α(D)-globin fragment from Triatoma infestans hindgut stimulates Trypanosoma cruzi adenylyl cyclase and promotes metacyclogenesis" . Biological Research 26, no. 1-2 (1993) : 279-283.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07169760_v26_n1-2_p279_Fraidenraich [ ]
---------- MLA ----------
Fraidenraich, D., Pena, C., Isola, E.L., Lammel, E.M., Coso, O., Diaz Anel, A., et al. "An α(D)-globin fragment from Triatoma infestans hindgut stimulates Trypanosoma cruzi adenylyl cyclase and promotes metacyclogenesis" . Biological Research, vol. 26, no. 1-2, 1993, pp. 279-283.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07169760_v26_n1-2_p279_Fraidenraich [ ]
---------- VANCOUVER ----------
Fraidenraich, D., Pena, C., Isola, E.L., Lammel, E.M., Coso, O., Diaz Anel, A., et al. An α(D)-globin fragment from Triatoma infestans hindgut stimulates Trypanosoma cruzi adenylyl cyclase and promotes metacyclogenesis. BIOL. RES. 1993;26(1-2):279-283.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07169760_v26_n1-2_p279_Fraidenraich [ ]
