Abstract:
We designed a set of procedures for achieving the tracking of cell nuclei and the identification of cell divisions in live zebrafish embryos using 3D+time images acquired by confocal laser scanning microscopy (CLSM). Our strategy includes image signal enhancement with feature preserving denoising algorithm, automated identification of the nuclei position, extraction of the optical flow from 3D images sequences and tracking of nuclei. ©2007 IEEE.
Registro:
Documento: |
Conferencia
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Título: | Cells tracking in a live zebrafish embryo |
Autor: | Melani, C.; Campana, M.; Lombardot, B.; Rizzi, B.; Veronesi, F.; Zanella, C.; Bourgine, P.; Mikula, K.; Peyriéras, N.; Sarti, A. |
Ciudad: | Lyon |
Filiación: | Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina Dipartimento di Elettronica, Informatica e Sistemistica, Università di Bologna, Italy DEPSN, Centre National de la Recherche Scientifique (CNRS), Institut de Neurobiologie Alfred Fessard, Gif-sur-Yvette, France Dept. of Mathematics and Descriptive Geometry, Slovak University of Technology, Slovakia Dipar. di Elettronica, Informatica e Sistemistica, Università di Bologna, Italy Centre de Recherche en Epistémologie Appliquée, CNRS École Polytechnique, Paris, France
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Palabras clave: | Algorithms; Cells; Confocal microscopy; Nucleic acids; Three dimensional; Nuclei position; Zebrafish; Fish; algorithm; animal; animal embryo; article; artificial intelligence; automated pattern recognition; computer assisted diagnosis; confocal microscopy; cytology; evaluation study; fluorescence microscopy; histology; image enhancement; methodology; prenatal development; reproducibility; sensitivity and specificity; three dimensional imaging; zebra fish; Algorithms; Animals; Artificial Intelligence; Embryo, Nonmammalian; Image Enhancement; Image Interpretation, Computer-Assisted; Imaging, Three-Dimensional; Microscopy, Confocal; Microscopy, Fluorescence; Pattern Recognition, Automated; Reproducibility of Results; Sensitivity and Specificity; Zebrafish |
Año: | 2007
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Página de inicio: | 1631
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Página de fin: | 1634
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DOI: |
http://dx.doi.org/10.1109/IEMBS.2007.4352619 |
Título revista: | 29th Annual International Conference of IEEE-EMBS, Engineering in Medicine and Biology Society, EMBC'07
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Título revista abreviado: | Annu Int Conf IEEE Eng Med Biol Proc
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ISSN: | 05891019
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CODEN: | CEMBA
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_05891019_v_n_p1631_Melani |
Referencias:
- Megason, S.G., Fraser, S.E., Digitizing life at the level of the cell: High-performance laser-scanning microscopy and image analysis for in toto imaging of development (2003) Mech Dev, 120 (11), pp. 1407-1420. , November
- Cooper, M., D'Amico, L., Henry, C., Confocal microscopic analysis of morphogenetic movements (1999) Methods in Cell Biology, 59 (7), pp. 179-204
- Dooley, K., Zon, L., Zebrafish: A model system for the study of human disease (2000) Curr Opin Genet Dev, 10 (3), pp. 252-256. , Jun
- Kimmel, C., Ballard, W., Kimmel, S., Ullmann, B., Schilling, T., Stages of embryonic development of the zebrafish (1995) Devi. Dyn, 203, pp. 253-310
- Perona, P., Malik, J., Scale-space and edge detection using anisotropic diffusion (1990) IEEE Trans. Pattern Anal. Mach. Intell, 12 (7), pp. 629-639
- Duda, R.O., Hart, P.E., Use of the hough transformation to detect lines and curves in pictures (1972) Commun. ACM, 15 (1), pp. 11-15
- Canny, J., A computational approach to edge detection (1986) IEEE Trans. Pattern Anal. Mach. Intell, 8 (6), pp. 679-698. , November
- Horn, B.K., Schunck, B.G., (1980) Determining optical flow, , Cambridge, MA, USA, Tech. Rep
- Sethian, J., (1998) Level set methods and fast marching methods: Evolving interfaces in computational geometry
- Vemuri, B.C., Ye, J., Chen, Y., Leonard, C.M., A level-set based approach to image registration (2000) MMBIA '00: Proceedings of the IEEE Workshop on Mathematical Methods in Biomedical Image Analysis, p. 86. , Washington, DC, USA: IEEE Computer SocietyA4 - ACIES, Research Promotion and Management Consulting; EOARD, European Office of Aerospace R and D; Grand Lyon; NSF, National Science Foundation; Philips Research Europe; Philips Research North America
Citas:
---------- APA ----------
Melani, C., Campana, M., Lombardot, B., Rizzi, B., Veronesi, F., Zanella, C., Bourgine, P.,..., Sarti, A.
(2007)
. Cells tracking in a live zebrafish embryo. 29th Annual International Conference of IEEE-EMBS, Engineering in Medicine and Biology Society, EMBC'07, 1631-1634.
http://dx.doi.org/10.1109/IEMBS.2007.4352619---------- CHICAGO ----------
Melani, C., Campana, M., Lombardot, B., Rizzi, B., Veronesi, F., Zanella, C., et al.
"Cells tracking in a live zebrafish embryo"
. 29th Annual International Conference of IEEE-EMBS, Engineering in Medicine and Biology Society, EMBC'07
(2007) : 1631-1634.
http://dx.doi.org/10.1109/IEMBS.2007.4352619---------- MLA ----------
Melani, C., Campana, M., Lombardot, B., Rizzi, B., Veronesi, F., Zanella, C., et al.
"Cells tracking in a live zebrafish embryo"
. 29th Annual International Conference of IEEE-EMBS, Engineering in Medicine and Biology Society, EMBC'07, 2007, pp. 1631-1634.
http://dx.doi.org/10.1109/IEMBS.2007.4352619---------- VANCOUVER ----------
Melani, C., Campana, M., Lombardot, B., Rizzi, B., Veronesi, F., Zanella, C., et al. Cells tracking in a live zebrafish embryo. Annu Int Conf IEEE Eng Med Biol Proc. 2007:1631-1634.
http://dx.doi.org/10.1109/IEMBS.2007.4352619