Artículo
Abstract:
Fruit softening has been largely associated with cell wall degradation by a number of loosening proteins. Firmness is a major quality attribute of fresh cherries and also an important factor affecting the susceptibility to postharvest rots. By analyzing the solubilization, depolymerization and composition of pectins and hemicelluloses in cultivars with contrasting firmness we found that the pattern and extent of their wall disassembly was quite similar. No marked pectin or hemicellulose depolymerization was observed and a similar reduction in tightlybound pectins and hemicellulose was detected in both cultivars during ripening. However, firm cherries presented pectic polymers with lower proportion of neutral sugars compared to uronic acids, suggesting that the variation in total wall polysaccharide and the branching of pectins assembled early in development or the proportion of homogalacturonan (HG) to type rhamnogalacturonan-I (RG-I) may contribute to the differences in firmness between cultivars. Dismantling of the cell wall by the action of relatively "well known" loosening agents is involved in the progressive softening occurring during ripening. Two of these proteins include polygalacturonases (PG) which are known to hydrolyze homogalacturonans and expansins (Exp) believed to participate in the relaxation of the cell wall by reducing hydrogen bonding between cellulose microfibrils and xyloglucan. We investigated the in vivo roles of these wall-disassembling proteins, by overexpressing PG and Exp1 both alone and in combination in a non-ripening rin tomato background. The simultaneous overexpression of PG and Exp1 in rin fruit restored softening in these non-ripening fruit. Unexpectedly, PG overexpression resulted in higher hemicellulose depolymerization while increased levels of Exp1 accelerated pectin turnover. This shows that besides their "well known" in vitro functions these proteins act in muro by facilitating the degradation of non-directly targeted wall components, likely by increasing the accessibility of pre-existing wall-degrading proteins to their polysaccharide substrates.
Registro:
| Documento: | Artículo |
| Título: | Role of "well known" proteins on cell wall degradation and softening |
| Autor: | Vicente, A.R.; Rodoni, L.M.; Salato, G.S.; Basanta, M.F.; Ponce, N.M.A.; Stortz, C.A.; Raffo, M.D.; Powell, A.L.T.; Bennett, A.B.; Labavitch, J.M.; Toivonen P.M.; Kalaitzis P.; Manganaris G.A. |
| Filiación: | CIDCA, Facultad de Ciencias Exactas, CONICET-UNLP, 47 and 116 y LIPA, Calle 60 y 119, La Plata, 1900, Argentina Departamento de Química Orgánica-CIHIDECAR-CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, Buenos Aires, 1428, Argentina Instituto Nacional de Tecnología Agropecuaria (INTA), EEA Alto Valle de Río Negro, Ruta Nac. 22, Km. 1190, Allen, 8332, Argentina Department of Plant Sciences, University of California, United States |
| Palabras clave: | Cell wall; Cherry; Expansin; Polygalacturonase; Softening; Tomato; Lycopersicon esculentum |
| Año: | 2015 |
| Volumen: | 1079 |
| Página de inicio: | 61 |
| Página de fin: | 69 |
| DOI: | http://dx.doi.org/10.17660/ActaHortic.2015.1079.5 |
| Título revista: | Acta Horticulturae |
| Título revista abreviado: | Acta Hortic. |
| ISSN: | 05677572 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_05677572_v1079_n_p61_Vicente |
Referencias:
- Barrett, D.M., Gonzalez, C., Activity of softening enzymes during cherry maturation (1994) J. Food Sci., 59, pp. 574-577
- Batisse, C., Fils-Lycaon, B., Buret, M., Pectin changes in ripening cherry fruit (1994) J. Food Sci., 59, pp. 389-393
- Batisse, C., Buret, M., Coulomb, P.J., Biochemical differences in cell wall of cherry fruit between soft and crisp fruit (1996) J. Agric. Food Chem., 44, pp. 453-457
- Batisse, C., Buret, M., Coulomb, P.J., Coulomb, C., Ultrastructure des parois de cerises Bigarreau Burlat de textures differentes au cours de la maturation (1996) Can. J. Bot., 74, pp. 1974-1981
- Brummell, D.A., Cell wall disassembly in ripening fruit (2006) Funct. Plant Biol., 33, pp. 103-119
- Brummell, D.A., Harpster, M.H., Cell wall metabolism in fruit softening and quality and its manipulation in transgenic plants (2001) Plant Mol. Biol., 47, pp. 311-340
- Cantu, D., Vicente, A.R., Dewey Bennett, A.B., Labavitch, J.M., Powell, A.L.T., The simultaneous suppression of tomato polygalacturonase and expansin reduces the susceptibility of ripe fruits to Botrytis cinerea (2008) Proc. Nat. Acad. Sci. USA, 105, pp. 859-864
- Carpita, N.C., McCann, M.C., The plant cell wall (2000) Biochemistry & Molecular Biology of Plants, pp. 52-108. , B. Buchanan, W. Gruissem and R. Jones eds., American Society of Plant Physiologists, Rockville, MD
- Choi, C., Toivonen, P., Wiersma, P.A., Kappel, F., Differences in levels of pectic substances and firmness in fruit from six sweet cherry genotypes (2002) J. Am. Pomol. Soc., 56, pp. 197-201
- Choi, C., Wiersma, P.A., Toivonen, P., Kappel, F., Fruit growth, firmness and cell wall hydrolytic enzyme activity during development of sweet cherry fruit treated with gibberellic acid (GA3) (2002) J. Hortic. Sci. Biotechnol., 77, pp. 615-621
- Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., Smith, F., Colorimetric method for determination of sugars and related substances (1956) Anal. Chem., 28, pp. 350-356
- Filisetti-Cozzi, T.M.C.C., Carpita, N.C., Measurement of uronic acids without interference from neutral sugars (1991) Anal. Biochem., 197, pp. 157-162
- Raffo, M.D., Ponce, N.M.A., Sozzi, G.O., Vicente, A.R., Stortz, C.A., Compositional changes in 'Bartlett' pear (Pyrus communis L.) cell wall polysaccharides as affected by sunlight conditions (2011) J. Agric. Food Chem., 59, pp. 12155-12162
- Vicente, A.R., Ortugno, C., Rosli, H., Powell, A.L.T., Greve, C.L., Labavitch, J.M., The temporal sequence of cell wall disassembly events in developing fruits: 2. Analysis of blueberry (Vaccinium sp.) (2007) J. Agric. Food Chem., 55, pp. 4119-4124
- Vicente, A.R., Ortugno, C., Powell, A.L.T., Greve, C.L., Labavitch, J.M., The temporal sequence of cell wall disassembly events in developing fruits: 1. Analysis of raspberry (Rubus idaeus) (2007) J. Agric. Food Chem., 55, pp. 4125-4130
- Vicente, A.R., Saladie, M., Rose, J., Labavitch, J., The linkage between cell wall metabolism and fruit softening: Looking to the future (2007) J. Sci. Food Agric., 87, pp. 1435-1448
Citas:
---------- APA ----------
Vicente, A.R., Rodoni, L.M., Salato, G.S., Basanta, M.F., Ponce, N.M.A., Stortz, C.A., Raffo, M.D.,..., Manganaris G.A. (2015) . Role of "well known" proteins on cell wall degradation and softening. Acta Horticulturae, 1079, 61-69.http://dx.doi.org/10.17660/ActaHortic.2015.1079.5
---------- CHICAGO ----------
Vicente, A.R., Rodoni, L.M., Salato, G.S., Basanta, M.F., Ponce, N.M.A., Stortz, C.A., et al. "Role of "well known" proteins on cell wall degradation and softening" . Acta Horticulturae 1079 (2015) : 61-69.http://dx.doi.org/10.17660/ActaHortic.2015.1079.5
---------- MLA ----------
Vicente, A.R., Rodoni, L.M., Salato, G.S., Basanta, M.F., Ponce, N.M.A., Stortz, C.A., et al. "Role of "well known" proteins on cell wall degradation and softening" . Acta Horticulturae, vol. 1079, 2015, pp. 61-69.http://dx.doi.org/10.17660/ActaHortic.2015.1079.5
---------- VANCOUVER ----------
Vicente, A.R., Rodoni, L.M., Salato, G.S., Basanta, M.F., Ponce, N.M.A., Stortz, C.A., et al. Role of "well known" proteins on cell wall degradation and softening. Acta Hortic. 2015;1079:61-69.http://dx.doi.org/10.17660/ActaHortic.2015.1079.5
