Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

The aim of the present study was to compare the effectiveness of the concentration techniques of flotation-centrifugation with zinc chloride (FZn) (δ = 1.45) with the spontaneous sedimentation (SSed) and the sucrose flotation-centrifugation (FSuc) (δ = 1.2) to recuperate gastrointestinal parasites from camelid fecal samples. The technique with more positive results for the detection of Nematodirus sp., Trichuris sp., strongyle-type eggs and Eimeria macusaniensis oocysts was the FZn. For Trichuris sp. and Eimeria macusaniensis, the higher coverglass counts were detected by FZn procedure. No significant differences were registered among centrifugation flotation techniques for Nematodirus spp. Coverglass count for strongyle-type eggs was significantly higher for FSuc than FZn (p = 0.0005) or SSed (p = 0.0005), being also significantly higher for FZn than for SSed (p = 0.008). FZn is a sensitive technique that allows the recovery of parasite elements with high density and it exerts low osmotic pressure avoiding parasite deformation. © 2014, Versita Warsaw and Springer-Verlag Wien.

Registro:

Documento: Artículo
Título:Effectiveness of coproscopic concentration techniques
Autor:Taglioretti, V.; Sardella, N.H.; Fugassa, M.H.
Filiación:Laboratorio de Paleoparasitología y Arqueología Contextual, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Funes 3350, Mar del Plata, Buenos Aires, Argentina
Palabras clave:camelid; Concentration techniques; parasite; zinc chloride
Año:2014
Volumen:51
Número:3
Página de inicio:210
Página de fin:214
DOI: http://dx.doi.org/10.2478/s11687-014-0231-x
Título revista:Helminthologia (Poland)
Título revista abreviado:Helminthologia
ISSN:04406605
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_04406605_v51_n3_p210_Taglioretti

Referencias:

  • Beldoménico, P.M., Uhart, M., Bono, M.F., Marull, C., Baldi, R., Peralta, J.L., Internal parasites of free-ranging guanacos from Patagonia (2003) Vet. Parasitol., 118, pp. 71-77. , PID: 14651877
  • Bowman, D.D., Lynn, R.C., Eberhard, M.L., Diagnostic parasitology (2003) Georgi’s parasitology for veterinarians, pp. 286-358. , Bowman D D, Lynn R C, Eberhard M L, (eds), WB Saunders Co, St Louis:
  • Cafrune, M.M., Aguirre, D.H., Richard, L.G., First report of Lamanema chavezi (Nematoda: Trichostrongyloidea) in llamas (Lama glama) from Argentina (2001) Vet. Parasitol., 97, pp. 165-168. , PID: 11358633, COI: 1:STN:280:DC%2BD3M3mvVWmug%3D%3D
  • Cafrune, M.M., Marín, R.E., Rigalt, F.A., Romero, S.R., Aguirre, D.H., Prevalence of Eimeria macusaniensis and Eimeria ivitaensis in South American camelids of Northwest Argentina (2009) Vet. Parasitol., 162, pp. 338-341. , PID: 19349120, COI: 1:STN:280:DC%2BD1MzlsFSlsw%3D%3D
  • Cebra, C.K., Stang, B.V., Comparison of methods to detect gastrointestinal parasites in llamas and alpacas (2008) J. Am. Vet. Med. Assoc., 232, pp. 733-741. , PID: 18312182
  • Cringoli, G., Rinaldi, L., Veneziano, V., Capelli, G., Scala, A., The influence of flotation solution, sample dilution and the choice of McMaster slide area (volume) on the reliability of the McMaster technique in estimating the faecal egg counts of gastrointestinal strongyles and Dicrocoelium dendriticum in sheep (2004) Vet. Parasitol., 123, pp. 121-131. , PID: 15265576, COI: 1:STN:280:DC%2BD2czmtlaktA%3D%3D
  • Cringoli, G., Rinaldi, L., Maurelli, M.L., Utzinger, J., FLOTAC: new multivalent techniques for qualitative and quantitative copromicroscopic diagnosis of parasites in animals and humans (2010) Nature Protocols, 5, pp. 503-515. , PID: 20203667, COI: 1:CAS:528:DC%2BC3cXislOmtrs%3D
  • Gordis, L., Assessing the validity and reliability of diagnostic and screening tests (2000) Epidemiology, pp. 63-81. , W.B. Saunders, Pennsylvania:
  • Gray, J., Extraction techniques (1965) Handbook of Paleontological Techniques, pp. 530-587. , Bernhard K, Raup D, (eds), Freeman, San Francisco & London:
  • Jarvinen, J.A., Prevalence of Eimeria macusaniensis (Apicomplexa: Eimeriidae) in Midwestern Lama spp (1999) J. Parasitol., 85, pp. 373-376. , PID: 10219324, COI: 1:STN:280:DyaK1M3js1Sltg%3D%3D
  • Kaminsky, R.G., (2003) Manual de parasitología. Métodos para laboratorios de atención primaria de salud
  • Leguía, G.P., The epidemiology and economic impact of llama parasites (1991) Parasitol. Today, 7, pp. 54-56. , PID: 15463422
  • Leguía, G.P., Casas, E., (1999) Enfermedades parasitarias y atlas parasitológico de camélidos sudamericanos, p. 191
  • Leguía, G.P., Casas, E., Eimeria ivitaensis n. sp. (Protozoa: Eimeriidae) en alpacas (Lama pacos) (1998) Rev. Per. Parasitol., 13, pp. 59-61
  • O’Grady, M.R., Slocombe, J.O.D., An investigation of variables in a fecal flotation technique (1980) Can. J. Comp. Med., 44, pp. 148-154. , PID: 7190861
  • Orr, R.J., Griffith, B.A., Champion, R.A., Cook, J.E., Defaecation and urination behaviour in beef cattle grazing semi-natural grassland (2012) Appl. Anim. Behav. Sci., 139, pp. 18-25
  • Quinn, R., Smith, H.V., Bruce, R.G., Girdwood, R.W.A., Studies on the incidence of Toxocara and Toxascaris spp. ova in the environment. 1. A comparison of flotation procedures for recovering Toxocara spp. ova from soil (1980) J. Hyg. Cambridge, 84, pp. 83-89. , COI: 1:STN:280:DyaL3c%2FpsFCisA%3D%3D
  • Rickard, L., Bishop, J., Redescription of Trichuris tenuis, Chandler, 1930, from llamas (Lama glama) in Oregon with a key to the species of Trichuris present in North American ruminants (1991) J. Parasitol., 77, pp. 70-75. , PID: 1992097, COI: 1:STN:280:DyaK3M7isVaisw%3D%3D
  • Sánchez-Rojas, G., Gallina, S., Mule deer (Odocoileus hemionus) density in a landscape element of the Chihuahuan Desert, Mexico (2000) J. Arid. Environ., 44, pp. 357-368
  • Taglioretti, V., Fugassa, M.H., Mayo Iñiguez, A., Sardella, N.H., Evaluación de la eficacia de la flotación con cloruro de zinc como método de enriquecimiento para la recuperación de huevos de enteroparásitos (2012) Rev. Arg. Parasitol., 1, p. 164
  • Zajac, A.M., Johnson, J., King, S.E., Evaluation of the importance of centrifugation as a component of zinc sulfate fecal flotation examinations (2002) J. Am. Anim. Hosp. Assoc., 38, pp. 221-224. , PID: 12022406

Citas:

---------- APA ----------
Taglioretti, V., Sardella, N.H. & Fugassa, M.H. (2014) . Effectiveness of coproscopic concentration techniques. Helminthologia (Poland), 51(3), 210-214.
http://dx.doi.org/10.2478/s11687-014-0231-x
---------- CHICAGO ----------
Taglioretti, V., Sardella, N.H., Fugassa, M.H. "Effectiveness of coproscopic concentration techniques" . Helminthologia (Poland) 51, no. 3 (2014) : 210-214.
http://dx.doi.org/10.2478/s11687-014-0231-x
---------- MLA ----------
Taglioretti, V., Sardella, N.H., Fugassa, M.H. "Effectiveness of coproscopic concentration techniques" . Helminthologia (Poland), vol. 51, no. 3, 2014, pp. 210-214.
http://dx.doi.org/10.2478/s11687-014-0231-x
---------- VANCOUVER ----------
Taglioretti, V., Sardella, N.H., Fugassa, M.H. Effectiveness of coproscopic concentration techniques. Helminthologia. 2014;51(3):210-214.
http://dx.doi.org/10.2478/s11687-014-0231-x