Artículo

Gritti, M.F.L.; Beviacqua, M.; Bordenave, R.H.; Rumi, L.S. "Interferon-alpha 2b modulation of doxorubicin sensitivity in a multidrug resistant cell line" (2001) Journal of Experimental and Clinical Cancer Research. 20(3):393-400
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Abstract:

We evaluated the effect of interferon-alpha2b as a chemosensitiser on HCT-15 cell line in treatment with doxorubicin. Chemosensitivity was determined by [3H]-thymidine incorporation and tetrazolium assays. The levels of expression of P-glycoprotein, Bcl-2 oncoprotein and HLA-ABC complex, and cell cycle/apoptosis analysis were determined by flow cytometry. Dox 50 ng/ml - IFN α 2b 500 IU/ml treatment inhibited cell proliferation (47.2 ± 1.4%, p< 0.0001; MTT assay: 40.6 ± 1.2%, p < 0.0001) and augmented the expression of P-170, Bcl-2 and HLA-ABC, while it didn't exert apoptosis, producing a slight G2/M arrest. A concentration of IFN-α2b, that by itself is not cytotoxic, can potentiate the efficacy of the anticancer drug. This effect is not due to a down-modulation of P-170. The absence of apoptosis and augmented levels of Bcl-2 expression suggests that this could be one of the mechanisms of drug resistance exerted by these cells.

Registro:

Documento: Artículo
Título:Interferon-alpha 2b modulation of doxorubicin sensitivity in a multidrug resistant cell line
Autor:Gritti, M.F.L.; Beviacqua, M.; Bordenave, R.H.; Rumi, L.S.
Filiación:Inst. Biologia/Medicina Experimental, Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina
Palabras clave:Apoptosis; Bcl-2; HLA-ABC; Interferon-alpha 2b; P-170; alpha2b interferon; doxorubicin; glycoprotein P; HLA antigen; protein bcl 2; tetrazolium; thymidine; tritium; apoptosis; article; cancer cell culture; cell cycle G2 phase; cell viability; concentration response; controlled study; drug potentiation; flow cytometry; gene expression; human; human cell; multidrug resistance; priority journal; Adenocarcinoma; Antineoplastic Agents; Apoptosis; Cell Cycle; Cell Division; Cell Survival; Colonic Neoplasms; Doxorubicin; Drug Resistance, Multiple; Drug Synergism; G2 Phase; Histocompatibility Antigens Class I; HLA Antigens; HLA-A Antigens; HLA-C Antigens; Humans; Interferon Alfa-2b; Membrane Proteins; Mitosis; Oncogene Protein v-cbl; P-Glycoprotein; Retroviridae Proteins, Oncogenic; Tumor Cells, Cultured
Año:2001
Volumen:20
Número:3
Página de inicio:393
Página de fin:400
Título revista:Journal of Experimental and Clinical Cancer Research
Título revista abreviado:J. Exp. Clin. Cancer Res.
ISSN:03929078
CODEN:JECRD
CAS:Antineoplastic Agents; Doxorubicin, 23214-92-8; HFE protein, human; Histocompatibility Antigens Class I; HLA Antigens; HLA-A Antigens; HLA-C Antigens; Interferon Alfa-2b, 99210-65-8; Membrane Proteins; Oncogene Protein v-cbl; P-Glycoprotein; Retroviridae Proteins, Oncogenic
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03929078_v20_n3_p393_Gritti

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Citas:

---------- APA ----------
Gritti, M.F.L., Beviacqua, M., Bordenave, R.H. & Rumi, L.S. (2001) . Interferon-alpha 2b modulation of doxorubicin sensitivity in a multidrug resistant cell line. Journal of Experimental and Clinical Cancer Research, 20(3), 393-400.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03929078_v20_n3_p393_Gritti [ ]
---------- CHICAGO ----------
Gritti, M.F.L., Beviacqua, M., Bordenave, R.H., Rumi, L.S. "Interferon-alpha 2b modulation of doxorubicin sensitivity in a multidrug resistant cell line" . Journal of Experimental and Clinical Cancer Research 20, no. 3 (2001) : 393-400.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03929078_v20_n3_p393_Gritti [ ]
---------- MLA ----------
Gritti, M.F.L., Beviacqua, M., Bordenave, R.H., Rumi, L.S. "Interferon-alpha 2b modulation of doxorubicin sensitivity in a multidrug resistant cell line" . Journal of Experimental and Clinical Cancer Research, vol. 20, no. 3, 2001, pp. 393-400.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03929078_v20_n3_p393_Gritti [ ]
---------- VANCOUVER ----------
Gritti, M.F.L., Beviacqua, M., Bordenave, R.H., Rumi, L.S. Interferon-alpha 2b modulation of doxorubicin sensitivity in a multidrug resistant cell line. J. Exp. Clin. Cancer Res. 2001;20(3):393-400.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03929078_v20_n3_p393_Gritti [ ]