Abstract:
Tumour necrosis factor-alpha (TNF-α) is a monocyte (MO)-derived cytokine that plays an essential role in the immunological system. In the present study our aim was to evaluate the levels of TNF-α secreted by MO from cancer patients. Blood MO were obtained from 10 lung cancer patients (LCP), 10 colorectal cancer patients (CCP) and 10 healthy donors (HD). TNF-α levels in MO culture supernatants spontaneously (sp) secreted or after stimulation with LPS treatment were evaluated using a commercial ELISA kit (sensibility: 10-1000 pg/ml). Mean values, expressed as pg/ml were: LCP: sp= 452.6 ± 107.2, LPS= 589.5 ± 126.7); CCP: sp= 84.1 ± 25.0, LPS= 437.3 ± 93.2; HD: sp= 74.2 ± 21.5, LPS= 573.5 ± 87.1. We concluded that MO from LCP secrete high levels of TNF-α spontaneously (p< 0.003 versus HD) and it was also observed an absence of response to LPS treatment in the 33% of the cases in these patients.
Registro:
Documento: |
Artículo
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Título: | Tumor necrosis factor-alfa production by monocytes from lung and colorectal cancer patients |
Autor: | Trejo, Y.G.; Bordenave, R.H.; Beviacqua, M.; Zanoni, L.; Rumi, L.S. |
Filiación: | Inst. de Biologia y Medicina Exp., Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina
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Palabras clave: | Colon cancer; Lung cancer; Monocytes; Tumor necrosis factor alfa; lipopolysaccharide; tumor necrosis factor alpha; article; clinical article; colorectal cancer; controlled study; cytokine production; cytokine release; enzyme linked immunosorbent assay; human; human cell; lung cancer; monocyte; mononuclear cell; priority journal; tumor immunology; Adult; Aged; Aged, 80 and over; Cells, Cultured; Colorectal Neoplasms; Enzyme-Linked Immunosorbent Assay; Humans; Lung Neoplasms; Middle Aged; Monocytes; Reference Values; Tumor Necrosis Factor-alpha |
Año: | 2001
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Volumen: | 20
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Número: | 1
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Página de inicio: | 71
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Página de fin: | 73
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Título revista: | Journal of Experimental and Clinical Cancer Research
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Título revista abreviado: | J. Exp. Clin. Cancer Res.
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ISSN: | 03929078
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CODEN: | JECRD
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CAS: | Tumor Necrosis Factor-alpha
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03929078_v20_n1_p71_Trejo |
Referencias:
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Citas:
---------- APA ----------
Trejo, Y.G., Bordenave, R.H., Beviacqua, M., Zanoni, L. & Rumi, L.S.
(2001)
. Tumor necrosis factor-alfa production by monocytes from lung and colorectal cancer patients. Journal of Experimental and Clinical Cancer Research, 20(1), 71-73.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03929078_v20_n1_p71_Trejo [ ]
---------- CHICAGO ----------
Trejo, Y.G., Bordenave, R.H., Beviacqua, M., Zanoni, L., Rumi, L.S.
"Tumor necrosis factor-alfa production by monocytes from lung and colorectal cancer patients"
. Journal of Experimental and Clinical Cancer Research 20, no. 1
(2001) : 71-73.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03929078_v20_n1_p71_Trejo [ ]
---------- MLA ----------
Trejo, Y.G., Bordenave, R.H., Beviacqua, M., Zanoni, L., Rumi, L.S.
"Tumor necrosis factor-alfa production by monocytes from lung and colorectal cancer patients"
. Journal of Experimental and Clinical Cancer Research, vol. 20, no. 1, 2001, pp. 71-73.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03929078_v20_n1_p71_Trejo [ ]
---------- VANCOUVER ----------
Trejo, Y.G., Bordenave, R.H., Beviacqua, M., Zanoni, L., Rumi, L.S. Tumor necrosis factor-alfa production by monocytes from lung and colorectal cancer patients. J. Exp. Clin. Cancer Res. 2001;20(1):71-73.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03929078_v20_n1_p71_Trejo [ ]