Artículo

Sisti, F.; Fernández, J.; Higgins, S.C.; Casabuono, A.; Couto, A.; Mills, K.H.G.; Hozbor, D. "A deep rough type structure in Bordetella bronchiseptica lipopolysaccharide modulates host immune responses" (2011) Microbiology and Immunology. 55(12):847-854
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Abstract:

The present authors have previously obtained the Bordetella bronchiseptica mutant BbLP39, which contains a deep-rough lipopolysaccharide (LPS) instead the wild type smooth LPS with O antigen. This mutant was found to be altered in the expression of some proteins and in its ability to colonize mouse lungs. Particularly, in BbLP39 the expression of pertactin is decreased. To differentiate the contribution of each bacterial component to the observed phenotype, here mice defective in the LPS sensing receptor TLR4 (TLR4-defective mice) were used. In contrast to wild-type mice, infection of TLR4-defective mice with BbLP39 resulted in lung infection, which persisted for more than 10 days post-challenge. Comparative analysis of the immune responses induced by purified mutant and wild type LPSs showed that the mutant LPS induced significantly higher degrees of expression of TNF-α and IL-10 mRNA than did the wild type. UV matrix-assisted laser desorption/ionization time-of-flight (MALDI TOF) mass spectrometry analysis revealed that both LPSs had the same penta-acylated lipid A structure. However, the lipid A from BbLP39 contained pyrophosphate instead of phosphate at position 1. This structural difference, in addition to the lack of O-antigen in BbLP39, may explain the functional differences between BbLP39 and wild type strains. © 2011 The Societies and Blackwell Publishing Asia Pty Ltd.

Registro:

Documento: Artículo
Título:A deep rough type structure in Bordetella bronchiseptica lipopolysaccharide modulates host immune responses
Autor:Sisti, F.; Fernández, J.; Higgins, S.C.; Casabuono, A.; Couto, A.; Mills, K.H.G.; Hozbor, D.
Filiación:VacSal Laboratory, Biotechnology and Molecular Biology Institute, Department of Biological Sciences, Faculty of Sciences, National University of La Plata, National Council of Scientific and Technical Research (CONICET), Calles 47 y 115, 1900 La Plata, Argentina
Center for Carbohydrate Research, Department of Organic Chemistry, Faculty of Exact and Natural Science, (1428) University of Buenos Aires, Buenos Aires, Argentina
Immune Regulation Research Group, School of Biochemistry and Immunology, Trinity College, Dublin 2, Ireland
Palabras clave:Bordetella bronchiseptica; Deep rough mutant; LPS; bacterium lipopolysaccharide; interleukin 10; lipid A; messenger RNA; tumor necrosis factor alpha; animal cell; animal experiment; animal model; article; bacterial strain; Bordetella bronchiseptica; Bordetella bronchiseptica infection; carbohydrate analysis; controlled study; female; gene expression; immune response; immunomodulation; in vitro study; in vivo study; lung infection; matrix assisted laser desorption ionization time of flight mass spectrometry; mouse; nonhuman; strain difference; structure analysis; wild type; Animals; Bordetella bronchiseptica; Bordetella Infections; Cytokines; Female; Lipid A; Lipopolysaccharides; Lung; Mice; Mice, Inbred BALB C; Mice, Inbred C3H; Mutation; O Antigens; Respiratory Tract Infections; Toll-Like Receptor 4; Bacteria (microorganisms); Bordetella bronchiseptica; Mus
Año:2011
Volumen:55
Número:12
Página de inicio:847
Página de fin:854
DOI: http://dx.doi.org/10.1111/j.1348-0421.2011.00395.x
Título revista:Microbiology and Immunology
Título revista abreviado:Microbiol. Immunol.
ISSN:03855600
CODEN:MIIMD
CAS:lipid A, 95991-05-2; Cytokines; Lipid A; Lipopolysaccharides; O Antigens; Toll-Like Receptor 4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03855600_v55_n12_p847_Sisti

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Citas:

---------- APA ----------
Sisti, F., Fernández, J., Higgins, S.C., Casabuono, A., Couto, A., Mills, K.H.G. & Hozbor, D. (2011) . A deep rough type structure in Bordetella bronchiseptica lipopolysaccharide modulates host immune responses. Microbiology and Immunology, 55(12), 847-854.
http://dx.doi.org/10.1111/j.1348-0421.2011.00395.x
---------- CHICAGO ----------
Sisti, F., Fernández, J., Higgins, S.C., Casabuono, A., Couto, A., Mills, K.H.G., et al. "A deep rough type structure in Bordetella bronchiseptica lipopolysaccharide modulates host immune responses" . Microbiology and Immunology 55, no. 12 (2011) : 847-854.
http://dx.doi.org/10.1111/j.1348-0421.2011.00395.x
---------- MLA ----------
Sisti, F., Fernández, J., Higgins, S.C., Casabuono, A., Couto, A., Mills, K.H.G., et al. "A deep rough type structure in Bordetella bronchiseptica lipopolysaccharide modulates host immune responses" . Microbiology and Immunology, vol. 55, no. 12, 2011, pp. 847-854.
http://dx.doi.org/10.1111/j.1348-0421.2011.00395.x
---------- VANCOUVER ----------
Sisti, F., Fernández, J., Higgins, S.C., Casabuono, A., Couto, A., Mills, K.H.G., et al. A deep rough type structure in Bordetella bronchiseptica lipopolysaccharide modulates host immune responses. Microbiol. Immunol. 2011;55(12):847-854.
http://dx.doi.org/10.1111/j.1348-0421.2011.00395.x