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Abstract:

The native cholinergic receptor that mediates synaptic transmission between olivocochlear fibers and outer hair cells of the cochlea is permeable to Ca2+ and is thought to be composed of both the α9 and the α10 cholinergic nicotinic subunits. The aim of the present work was to study the permeability of the recombinant α9α10 nicotinic acetylcholine receptor to Ca2+, Ba2+ and Mg2+ and its modulation by these divalent cations. Experiments were performed, by the two-electrode voltage-clamp technique, in Xenopus laevis oocytes injected with α9 and α10 cRNA. The relative divalent to monovalent cation permeability was high (∼10) for Ca2+, Ba2+ and Mg2+. Currents evoked by acetylcholine (ACh) were potentiated by either Ca2+ or Ba2+ up to 500 μM but were blocked by higher concentrations of these cations. Potentiation by Ca2+ was voltage-independent, whereas blockage was stronger at hyperpolarized than at depolarized potentials. Mg2+ did not potentiate but it blocked ACh-evoked currents (IC50=0.38 mM). In the absence of Ca2+, the EC50 for ACh was higher (48 μM) than that obtained with 1.8 mM Ca2+ (14.3 μM), suggesting that potentiation by Ca2+ involves changes in the apparent affinity of the α9α10 receptor for ACh. © 2002 Elsevier Science B.V. All rights reserved.

Registro:

Documento: Artículo
Título:The α9α10 nicotinic acetylcholine receptor is permeable to and is modulated by divalent cations
Autor:Weisstaub, N.; Vetter, D.E.; Belén Elgoyhen, A.; Katz, E.
Filiación:Instituto De Investigaciones En Ingeniería Genética Y Biología Molecular, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Tufts University, School of Medicine, Boston, MA, United States
Departamento De Biología, FCEyN, UBA, Buenos Aires, Argentina
Palabras clave:Ca2+ permeability; Ligand-gated channel; Neurotransmitter receptor channel; Nicotinic receptor; Olivocochlear efferent synapse; Outer hair cell; Voltage-dependent blockage; acetylcholine; barium ion; calcium ion; complementary RNA; divalent cation; magnesium ion; monovalent cation; nicotinic receptor; receptor subunit; recombinant receptor; calcium; Chrna9 protein, rat; divalent cation; magnesium; protein subunit; recombinant protein; article; concentration response; controlled study; depolarization; electric potential; electrode; evoked response; hyperpolarization; IC 50; ion current; ion permeability; modulation; nonhuman; oocyte; priority journal; rat; receptor affinity; receptor blocking; regulatory mechanism; voltage clamp; Xenopus laevis; animal; chemistry; drug effect; electrophysiology; female; genetics; hair cell; in vitro study; metabolism; permeability; synaptic transmission; Animals; Calcium; Cations, Divalent; Electrophysiology; Female; Hair Cells, Outer; Magnesium; Oocytes; Permeability; Protein Subunits; Rats; Receptors, Nicotinic; Recombinant Proteins; Synaptic Transmission; Xenopus laevis
Año:2002
Volumen:167
Número:1-2
Página de inicio:122
Página de fin:135
DOI: http://dx.doi.org/10.1016/S0378-5955(02)00380-5
Título revista:Hearing Research
Título revista abreviado:Hear. Res.
ISSN:03785955
CODEN:HERED
CAS:Calcium, 7440-70-2; Cations, Divalent; Chrna9 protein, rat; Magnesium, 7439-95-4; Protein Subunits; Receptors, Nicotinic; Recombinant Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03785955_v167_n1-2_p122_Weisstaub

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Citas:

---------- APA ----------
Weisstaub, N., Vetter, D.E., Belén Elgoyhen, A. & Katz, E. (2002) . The α9α10 nicotinic acetylcholine receptor is permeable to and is modulated by divalent cations. Hearing Research, 167(1-2), 122-135.
http://dx.doi.org/10.1016/S0378-5955(02)00380-5
---------- CHICAGO ----------
Weisstaub, N., Vetter, D.E., Belén Elgoyhen, A., Katz, E. "The α9α10 nicotinic acetylcholine receptor is permeable to and is modulated by divalent cations" . Hearing Research 167, no. 1-2 (2002) : 122-135.
http://dx.doi.org/10.1016/S0378-5955(02)00380-5
---------- MLA ----------
Weisstaub, N., Vetter, D.E., Belén Elgoyhen, A., Katz, E. "The α9α10 nicotinic acetylcholine receptor is permeable to and is modulated by divalent cations" . Hearing Research, vol. 167, no. 1-2, 2002, pp. 122-135.
http://dx.doi.org/10.1016/S0378-5955(02)00380-5
---------- VANCOUVER ----------
Weisstaub, N., Vetter, D.E., Belén Elgoyhen, A., Katz, E. The α9α10 nicotinic acetylcholine receptor is permeable to and is modulated by divalent cations. Hear. Res. 2002;167(1-2):122-135.
http://dx.doi.org/10.1016/S0378-5955(02)00380-5