Artículo

Montanari, J.; Perez, A.P.; Di Salvo, F.; Diz, V.; Barnadas, R.; Dicelio, L.; Doctorovich, F.; Morilla, M.J.; Romero, E.L. "Photodynamic ultradeformable liposomes: Design and characterization" (2007) International Journal of Pharmaceutics. 330(1-2):183-194
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Abstract:

Hydrophobic ([tetrakis(2,4-dimetil-3-pentyloxi)-phthalocyaninate]zinc(II)) (ZnPc) and hydrophilic ([tetrakis(N,N,N-trimethylammoniumetoxi)-phthalocyaninate]zinc(II) tetraiodide) (ZnPcMet) phthalocyanines were synthesized and loaded in ultradeformable liposomes (UDL) of soybean phosphatidylcholine and sodium cholate (6:1, w/w, ratio), resulting 100 nm mean size vesicles of negative Zeta potential, with encapsulation efficiencies of 85 and 53%, enthalpy of phase transition of 5.33 and 158 J/mmol for ZnPc and ZnPcMet, respectively, indicating their deep and moderate partition into UD matrices. Matrix elasticity of UDL-phthalocyanines resulted 28-fold greater than that of non-UDL, leaking only 25% of its inner aqueous content after passage through a nanoporous barrier versus 100% leakage for non-UDL. UDL-ZnPc made ZnPc soluble in aqueous buffer while kept the monomeric state, rendering singlet oxygen quantum yield (ΦΔ) similar to that obtained in ethanol (0.61), whereas UDL-ZnPcMet had a four-fold higher ΦΔ than that of free ZnPcMet (0.21). Free phthalocyanines were non-toxic at 1 and 10 μM, both in dark or upon irradiation at 15 J/cm2 on Vero and J-774 cells (MTT assay). Only liposomal ZnPc at 10 μM was toxic for J-774 cells under both conditions. Aditionally, endo-lysosomal confinement of the HPTS dye was kept after irradiation at 15 J/cm2 in the presence of UDL-phtalocyanines. This could lead to improve effects of singlet oxygen against intra-vesicular pathogen targets inside the endo-lysosomal system. © 2006 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Photodynamic ultradeformable liposomes: Design and characterization
Autor:Montanari, J.; Perez, A.P.; Di Salvo, F.; Diz, V.; Barnadas, R.; Dicelio, L.; Doctorovich, F.; Morilla, M.J.; Romero, E.L.
Filiación:Laboratorio de Diseño de Estrategias de Targeting de Drogas (LDTD), Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Saenz Peña 180, Bernal, B1876BXD Buenos Aires, Argentina
Departamento de Química Inorgánica, Analítica y Química Física/INQUIMAE-CONICET, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Pabellon II, Argentina
Centre de Estudis Biofísics, Universitat Autónoma de Barcelona, 08193 Cerdanyola, Catalunya, Spain
Departament de Físicaquímica, Facultat de Farmácia, Universitat de Barcelona, Av. Joan XXIII, s/n, 08028 Barcelona, Catalunya, Spain
Palabras clave:Phthalocyanines; Ultradeformable liposomes; cholic acid; liposome; oxidoreductase; phosphatidylcholine; phthalocyanine derivative; tetrakis(2,4 dimethyl 3 pentyloxy)phthalocyaninate zinc; tetrakis(n,n,n trimethylammoniumethoxy)phthalocyaninate zinc tetraiodide; unclassified drug; absorption spectroscopy; animal cell; article; controlled study; differential scanning calorimetry; drug cytotoxicity; drug delivery system; drug structure; encapsulation; fluorescence microscopy; hydrophilicity; hydrophobicity; nonhuman; phase transition; photodynamics; priority journal; quantum yield; thermodynamics; transmission electron microscopy; Vero cell; zeta potential; Animals; Calorimetry, Differential Scanning; Cell Survival; Cercopithecus aethiops; Indoles; Leishmaniasis, Cutaneous; Liposomes; Macrophages; Mice; Microscopy, Electron, Transmission; Nanotechnology; Photochemotherapy; Radiation-Sensitizing Agents; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Vero Cells
Año:2007
Volumen:330
Número:1-2
Página de inicio:183
Página de fin:194
DOI: http://dx.doi.org/10.1016/j.ijpharm.2006.11.015
Título revista:International Journal of Pharmaceutics
Título revista abreviado:Int. J. Pharm.
ISSN:03785173
CODEN:IJPHD
CAS:cholic acid, 32500-01-9, 361-09-1, 81-25-4; oxidoreductase, 9035-73-8, 9035-82-9, 9037-80-3, 9055-15-6; phosphatidylcholine, 55128-59-1, 8002-43-5; Indoles; Liposomes; Radiation-Sensitizing Agents; phthalocyanine, 574-93-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03785173_v330_n1-2_p183_Montanari

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Citas:

---------- APA ----------
Montanari, J., Perez, A.P., Di Salvo, F., Diz, V., Barnadas, R., Dicelio, L., Doctorovich, F.,..., Romero, E.L. (2007) . Photodynamic ultradeformable liposomes: Design and characterization. International Journal of Pharmaceutics, 330(1-2), 183-194.
http://dx.doi.org/10.1016/j.ijpharm.2006.11.015
---------- CHICAGO ----------
Montanari, J., Perez, A.P., Di Salvo, F., Diz, V., Barnadas, R., Dicelio, L., et al. "Photodynamic ultradeformable liposomes: Design and characterization" . International Journal of Pharmaceutics 330, no. 1-2 (2007) : 183-194.
http://dx.doi.org/10.1016/j.ijpharm.2006.11.015
---------- MLA ----------
Montanari, J., Perez, A.P., Di Salvo, F., Diz, V., Barnadas, R., Dicelio, L., et al. "Photodynamic ultradeformable liposomes: Design and characterization" . International Journal of Pharmaceutics, vol. 330, no. 1-2, 2007, pp. 183-194.
http://dx.doi.org/10.1016/j.ijpharm.2006.11.015
---------- VANCOUVER ----------
Montanari, J., Perez, A.P., Di Salvo, F., Diz, V., Barnadas, R., Dicelio, L., et al. Photodynamic ultradeformable liposomes: Design and characterization. Int. J. Pharm. 2007;330(1-2):183-194.
http://dx.doi.org/10.1016/j.ijpharm.2006.11.015