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Abstract:

A novel biospecific affinity chromatographic procedure was developed for the purification of the sex steroid binding protein from Bufo arenarum. A charcoal column connected in series to the affinity column allows the removal of any ligand non-covalently bound to the matrix or released during its storage, thus avoiding the need for exhaustive and prolonged washing procedures. In addition, it is not necessary to remove the endogenous ligand from the starting material and the binding to the affinity column can be monitored to determine the time required to achieve the maximum yield. The advantages are the charcoal adsorption of the ligand "washed" from the affinity column by the protein to be purified and the amplification provided by the cyclic use of the system. The procedure improves the yield from less than 1% (by conventional procedures) to more than 50%. With minor modifications this procedure can be useful for the purification of binding proteins and receptors. © 1987.

Registro:

Documento: Artículo
Título:Cyclic biospecific affinity chromatographic method for the purification of the sex steroid binding protein (SBP): Application to the purification of SBP from toad
Autor:Santa-Coloma, T.A.; Garraffo, H.M.; Gros, E.G.; Charreau, E.H.
Filiación:Instituto de Biologia y Medicina Experimental, Obligado 2490, Buenos Aires, 1428, Argentina
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pab. 2, Buenos Aires, 1428, Argentina
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pab. 2, Buenos Aires, 1428, Argentina
Instituto de Biologia y Medicina Experimental, Obligado 2490, Buenos Aires, 1428, Argentina
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pab. 2, Buenos Aires, 1428, Argentina
Palabras clave:sex hormone binding protein; affinity chromatography; animal cell; methodology; nonhuman; priority journal; protein purification; toad; Ammonium Sulfate; Androstane-3,17-diol; Animal; Bufo arenarum; Carrier Proteins; Chromatography, Affinity; Electrophoresis, Polyacrylamide Gel; Indicators and Reagents; Protein Binding; Sex Hormone-Binding Globulin; Stanolone
Año:1987
Volumen:415
Número:C
Página de inicio:297
Página de fin:304
DOI: http://dx.doi.org/10.1016/S0378-4347(00)83221-1
Título revista:Journal of Chromatography B: Biomedical Sciences and Applications
Título revista abreviado:J. Chromatogr. B Biomed. Sci. Appl.
ISSN:03784347
CODEN:JCBAD
CAS:Ammonium Sulfate, 7783-20-2; Androstane-3,17-diol, 25126-76-5; Carrier Proteins; Indicators and Reagents; Sex Hormone-Binding Globulin; Stanolone, 521-18-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03784347_v415_nC_p297_SantaColoma

Referencias:

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  • Rosenbaum, Christy, Kelly, (1966) J. Clin. Endocrinol. Metab., 26, p. 1399
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Citas:

---------- APA ----------
Santa-Coloma, T.A., Garraffo, H.M., Gros, E.G. & Charreau, E.H. (1987) . Cyclic biospecific affinity chromatographic method for the purification of the sex steroid binding protein (SBP): Application to the purification of SBP from toad. Journal of Chromatography B: Biomedical Sciences and Applications, 415(C), 297-304.
http://dx.doi.org/10.1016/S0378-4347(00)83221-1
---------- CHICAGO ----------
Santa-Coloma, T.A., Garraffo, H.M., Gros, E.G., Charreau, E.H. "Cyclic biospecific affinity chromatographic method for the purification of the sex steroid binding protein (SBP): Application to the purification of SBP from toad" . Journal of Chromatography B: Biomedical Sciences and Applications 415, no. C (1987) : 297-304.
http://dx.doi.org/10.1016/S0378-4347(00)83221-1
---------- MLA ----------
Santa-Coloma, T.A., Garraffo, H.M., Gros, E.G., Charreau, E.H. "Cyclic biospecific affinity chromatographic method for the purification of the sex steroid binding protein (SBP): Application to the purification of SBP from toad" . Journal of Chromatography B: Biomedical Sciences and Applications, vol. 415, no. C, 1987, pp. 297-304.
http://dx.doi.org/10.1016/S0378-4347(00)83221-1
---------- VANCOUVER ----------
Santa-Coloma, T.A., Garraffo, H.M., Gros, E.G., Charreau, E.H. Cyclic biospecific affinity chromatographic method for the purification of the sex steroid binding protein (SBP): Application to the purification of SBP from toad. J. Chromatogr. B Biomed. Sci. Appl. 1987;415(C):297-304.
http://dx.doi.org/10.1016/S0378-4347(00)83221-1