Artículo

Ramirez, M.C.; Bourguignon, N.S.; Bonaventura, M.M.; Lux-Lantos, V.; Libertun, C.; Becu-Villalobos, D. "Neonatal xenoestrogen exposure alters growth hormone-dependent liver proteins and genes in adult female rats" (2012) Toxicology Letters. 213(3):325-331
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Abstract:

The hypothalamic-growth hormone (GH)-liver axis represents a new concept in endocrine regulation of drug toxicity. Preponderant sex differences are found in liver gene expression, mostly dependent on the sexually dimorphic pattern of GH secretion which is set during the neonatal period by gonadal steroids. We tested if GH-dependent sexually dimorphic liver enzymes and proteins was perturbed by neonatal Bisphenol A (BPA) treatment in female rats. Female rats were sc injected with BPA (50 or 500μg/50μl) or castor oil vehicle from postnatal day 1 to 10. At five months serum prolactin, pituitary GH, and serum and liver insulin growth factor-I (IGF-I) were measured by RIA. Major urinary proteins (MUPs) were determined by electrophoresis. Liver Cyp2c11, Cyp2c12, Adh1, Hnf6, and Prlr mRNA levels were determined by real time PCR. Pituitary GH content and liver IGF-I concentration were increased by neonatal BPA treatment, indicating partial masculinization of the GH axis in treated females. GH-dependent female predominant liver enzyme genes (Cyp2c12 and Adh1) and a transcription factor (Hnf6) were downregulated or defeminized, while there were no changes in a male predominant gene (Cyp2c11) or protein (MUP). Our findings indicate that perinatal exposure to BPA may compromise the sexually dimorphic capacity of the liver to metabolize drugs and steroids. © 2012 Elsevier Ireland Ltd.

Registro:

Documento: Artículo
Título:Neonatal xenoestrogen exposure alters growth hormone-dependent liver proteins and genes in adult female rats
Autor:Ramirez, M.C.; Bourguignon, N.S.; Bonaventura, M.M.; Lux-Lantos, V.; Libertun, C.; Becu-Villalobos, D.
Filiación:Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:Bisphenol; Cyps; Growth hormone; Liver; Prolactin; Sexual dimorphism; 4,4' isopropylidenediphenol; alcohol dehydrogenase; castor oil; complementary DNA; cytochrome P450 2C11; cytochrome P450 2C12; endocrine disruptor; glyceraldehyde 3 phosphate dehydrogenase; growth hormone; liver enzyme; messenger RNA; prolactin; prolactin receptor; RNA; somatomedin C; unclassified drug; xenoestrogen; animal experiment; animal tissue; article; controlled study; detoxification; electrophoresis; environmental exposure; gene expression; growth hormone release; hormone blood level; hypothalamus; liver; liver metabolism; mouse; newborn; nonhuman; perinatal period; polyacrylamide gel electrophoresis; priority journal; protein urine level; radioimmunoassay; real time polymerase chain reaction; Age Factors; Aging; Alcohol Dehydrogenase; Animals; Animals, Newborn; Aryl Hydrocarbon Hydroxylases; Drug Administration Schedule; Electrophoresis, Polyacrylamide Gel; Endocrine Disruptors; Estrogens, Non-Steroidal; Female; Gene Expression Regulation, Developmental; Gene Expression Regulation, Enzymologic; Growth Hormone; Hepatocyte Nuclear Factor 6; Injections, Subcutaneous; Insulin-Like Growth Factor I; Liver; Male; Phenols; Pituitary Gland; Prolactin; Rats; Rats, Sprague-Dawley; Real-Time Polymerase Chain Reaction; Receptors, Prolactin; RNA, Messenger; Sex Characteristics; Sex Factors; Steroid 16-alpha-Hydroxylase; Steroid Hydroxylases; Rattus
Año:2012
Volumen:213
Número:3
Página de inicio:325
Página de fin:331
DOI: http://dx.doi.org/10.1016/j.toxlet.2012.07.015
Título revista:Toxicology Letters
Título revista abreviado:Toxicol. Lett.
ISSN:03784274
CODEN:TOLED
CAS:4,4' isopropylidenediphenol, 80-05-7; RNA, 63231-63-0; alcohol dehydrogenase, 9031-72-5; castor oil, 8001-79-4; glyceraldehyde 3 phosphate dehydrogenase, 9001-50-7; growth hormone, 36992-73-1, 37267-05-3, 66419-50-9, 9002-72-6; prolactin, 12585-34-1, 50647-00-2, 9002-62-4; somatomedin C, 67763-96-6; Alcohol Dehydrogenase, 1.1.1.1; Aryl Hydrocarbon Hydroxylases, 1.14.14.1; CYP2C11 protein, rat, 1.14.14.1; Endocrine Disruptors; Estrogens, Non-Steroidal; Growth Hormone, 9002-72-6; Hepatocyte Nuclear Factor 6; Insulin-Like Growth Factor I, 67763-96-6; Onecut1 protein, rat; Phenols; Prolactin, 9002-62-4; RNA, Messenger; Receptors, Prolactin; Steroid 16-alpha-Hydroxylase, 1.14.14.1; Steroid Hydroxylases, 1.14.-; bisphenol A, 80-05-7; insulin-like growth factor-1, rat; steroid 15-beta-hydroxylase, 1.14.14.1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03784274_v213_n3_p325_Ramirez

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Citas:

---------- APA ----------
Ramirez, M.C., Bourguignon, N.S., Bonaventura, M.M., Lux-Lantos, V., Libertun, C. & Becu-Villalobos, D. (2012) . Neonatal xenoestrogen exposure alters growth hormone-dependent liver proteins and genes in adult female rats. Toxicology Letters, 213(3), 325-331.
http://dx.doi.org/10.1016/j.toxlet.2012.07.015
---------- CHICAGO ----------
Ramirez, M.C., Bourguignon, N.S., Bonaventura, M.M., Lux-Lantos, V., Libertun, C., Becu-Villalobos, D. "Neonatal xenoestrogen exposure alters growth hormone-dependent liver proteins and genes in adult female rats" . Toxicology Letters 213, no. 3 (2012) : 325-331.
http://dx.doi.org/10.1016/j.toxlet.2012.07.015
---------- MLA ----------
Ramirez, M.C., Bourguignon, N.S., Bonaventura, M.M., Lux-Lantos, V., Libertun, C., Becu-Villalobos, D. "Neonatal xenoestrogen exposure alters growth hormone-dependent liver proteins and genes in adult female rats" . Toxicology Letters, vol. 213, no. 3, 2012, pp. 325-331.
http://dx.doi.org/10.1016/j.toxlet.2012.07.015
---------- VANCOUVER ----------
Ramirez, M.C., Bourguignon, N.S., Bonaventura, M.M., Lux-Lantos, V., Libertun, C., Becu-Villalobos, D. Neonatal xenoestrogen exposure alters growth hormone-dependent liver proteins and genes in adult female rats. Toxicol. Lett. 2012;213(3):325-331.
http://dx.doi.org/10.1016/j.toxlet.2012.07.015