Artículo

Rodriguez Ferreiro, G.; Cancino Badóas, L.; Lopez-Nigro, M.; Palermo, A.; Mudry, M.; González Elio, P.; Carballo, M.A. "DNA single strand breaks in peripheral blood lymphocytes induced by three nitroimidazole derivatives" (2002) Toxicology Letters. 132(2):109-115
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Abstract:

Tinidazole (TNZ), ornidazole (ONZ) and metronidazole (MTZ) are antiparasitic drugs (nitroimidazole derivatives) that have proven to be effective against Trichomonas vaginalis, Entoamoeba histolytica, Giardia lamblia and Helicobacter pylori. The reduction of the nitro group and the generation of short-lived reactive intermediates are the basis of its parasiticidal activity. This reduction is associated with its mutagenic activity in bacteria, although in mammalian cells DNA damage seems to be related to the production of reactive oxygen species (ROS). Using alkaline single cell electrophoresis, a significant increase in single strand breaks and alkali labile sites in human peripheral blood lymphocytes (PBL) exposed to MTZ, ONZ and TNZ at 10, 100 and 500 μg/ml is observed. MTZ causes less damage, especially at higher concentrations, when compared with TNZ, the most harmful of the drugs tested. These findings suggest that primary damage is induced under aerobic conditions and confirms that these nitroimidazoles are DNA damaging agents. © 2002 Elsevier Science Ireland Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:DNA single strand breaks in peripheral blood lymphocytes induced by three nitroimidazole derivatives
Autor:Rodriguez Ferreiro, G.; Cancino Badóas, L.; Lopez-Nigro, M.; Palermo, A.; Mudry, M.; González Elio, P.; Carballo, M.A.
Filiación:Grupo De Genética Toxicologóa Y Salud Ambiental, Centro De Investigaciones Biomeédicas, Instituto Superior De Ciencias Medicas De La Habana, Havana, Cuba
Citogenética Y Genética Toxicológica (CIGETOX), Depto. De Bioquímica Clínica, 1113-Ciudad De Buenos Aries, Junín 956, Buenos Aires, Argentina
Grupo De Investigaciones En Biología Evolutiva (GIBE), Facultad De Cs. Exactas Y Naturales, UBA, Buenos Aires, Argentina
CITEFA (Instituto De Investigaciones Científicas Y Teécnicas De Las FFAA), Buenos Aires, Argentina
Palabras clave:Comet assay; DNA damage; Metronidazole; Nitroimidazoles; Ornidazole; Tinidazole; DNA; metronidazole; nitroimidazole derivative; ornidazole; reactive oxygen metabolite; tinidazole; aerobic metabolism; antimicrobial activity; article; bacterial cell; comet assay; concentration response; controlled study; DNA damage; DNA strand breakage; drug cytotoxicity; human; human cell; lymphocyte; mammal cell; mutagenic activity; priority journal; Adult; Antitrichomonal Agents; Cells, Cultured; Comet Assay; DNA Damage; DNA, Single-Stranded; Dose-Response Relationship, Drug; Humans; Lymphocytes; Male; Metronidazole; Nitroimidazoles; Ornidazole; Tinidazole; Giardia; Giardia intestinalis; Helicobacter; Helicobacter pylori; Mammalia; Trichomonas vaginalis
Año:2002
Volumen:132
Número:2
Página de inicio:109
Página de fin:115
DOI: http://dx.doi.org/10.1016/S0378-4274(02)00039-5
Título revista:Toxicology Letters
Título revista abreviado:Toxicol. Lett.
ISSN:03784274
CAS:Antitrichomonal Agents; DNA, Single-Stranded; Metronidazole, 443-48-1; Nitroimidazoles; Ornidazole, 16773-42-5; Tinidazole, 19387-91-8
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03784274_v132_n2_p109_RodriguezFerreiro

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Citas:

---------- APA ----------
Rodriguez Ferreiro, G., Cancino Badóas, L., Lopez-Nigro, M., Palermo, A., Mudry, M., González Elio, P. & Carballo, M.A. (2002) . DNA single strand breaks in peripheral blood lymphocytes induced by three nitroimidazole derivatives. Toxicology Letters, 132(2), 109-115.
http://dx.doi.org/10.1016/S0378-4274(02)00039-5
---------- CHICAGO ----------
Rodriguez Ferreiro, G., Cancino Badóas, L., Lopez-Nigro, M., Palermo, A., Mudry, M., González Elio, P., et al. "DNA single strand breaks in peripheral blood lymphocytes induced by three nitroimidazole derivatives" . Toxicology Letters 132, no. 2 (2002) : 109-115.
http://dx.doi.org/10.1016/S0378-4274(02)00039-5
---------- MLA ----------
Rodriguez Ferreiro, G., Cancino Badóas, L., Lopez-Nigro, M., Palermo, A., Mudry, M., González Elio, P., et al. "DNA single strand breaks in peripheral blood lymphocytes induced by three nitroimidazole derivatives" . Toxicology Letters, vol. 132, no. 2, 2002, pp. 109-115.
http://dx.doi.org/10.1016/S0378-4274(02)00039-5
---------- VANCOUVER ----------
Rodriguez Ferreiro, G., Cancino Badóas, L., Lopez-Nigro, M., Palermo, A., Mudry, M., González Elio, P., et al. DNA single strand breaks in peripheral blood lymphocytes induced by three nitroimidazole derivatives. Toxicol. Lett. 2002;132(2):109-115.
http://dx.doi.org/10.1016/S0378-4274(02)00039-5