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Abstract:

Interleukin 1 (IL-1) is possibly one factor produced by the embryo that might have a role in the maternal immunological recognition of pregnancy. The purpose of this study was to identify an embryo-related factor suitable for prediction of pregnancy during IVF procedures. For this purpose, IL-1β levels were measured in 21 samples of human embryo culture-conditioned media. The average number of embryos per sample was 5 ± 1. Simultaneously, 16 cell culture media containing 10% autologous serum but no embryos were tested as controls. IL-1β levels were measured using the ELISA technique, and the biological activity of IL-1 was measured by means of a C3H/HeJ mice thymocyte proliferation assay. The average IL-1β level ± S.E.M. was 49 ± 7 pg/ml in embryo culture-conditioned media and 12 ± 2 pg/ml in controls (P < 0.001). The average IL-1β level in embryo culture-conditioned media from viable pregnancy cycles was 82 ± 6 pg/ml (n = 8), while in those cases that did not result in viable pregnancies the IL-1β level was significantly lower (28 ± 4 pg/ml, (n = 13, P < 0.001). The IL-1 activity of embryo culture-conditioned media, measured by [3H]thymidine incorporation in thymocytes was increased, compared with control media (442 ± 51 counts/min vs. 337 ± 13 counts/min, P < 0.05), and the highest values corresponded to media containing those embryos that resulted in pregnancies (589 ± 41 counts/min, P < 0.01 vs. controls). We conclude that the determination of the levels of this cytokine in embryo culture-conditioned media might be a predictive parameter for pregnancies in patients undergoing IVF-ET.

Registro:

Documento: Artículo
Título:Interleukin-1β levels in human embryo culture supernatants and their predictive value for pregnancy
Autor:Barañao, R.I.; Piazza, A.; Rumi, L.S.; Polak De Fried, E.
Filiación:Inst. de Biol. y Med. Experimental, CONICET, Obligado 2490, 1428 - Buenos Aires, Argentina
Centro de Salud Reproductiva (CER), Humbolt 2267, 1425 - Buenos Aires, Argentina
Palabras clave:Embryo culture-conditioned medium; Interleukin 1β; IVF; Pregnancy; interleukin 1beta; antigen recognition; article; cell proliferation; controlled study; culture medium; dna synthesis; embryo; embryo culture; enzyme linked immunosorbent assay; fertilization in vitro; human; prediction; pregnancy; supernatant; thymocyte; Culture Media, Conditioned; Culture Techniques; Embryo; Embryo Transfer; Female; Fertilization in Vitro; Humans; Interleukin-1; Pregnancy; Prospective Studies
Año:1997
Volumen:48
Número:1-2
Página de inicio:71
Página de fin:80
DOI: http://dx.doi.org/10.1016/S0378-3782(96)01837-3
Título revista:Early Human Development
Título revista abreviado:EARLY HUM. DEV.
ISSN:03783782
CODEN:EHDED
CAS:Culture Media, Conditioned; Interleukin-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03783782_v48_n1-2_p71_Baranao

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Citas:

---------- APA ----------
Barañao, R.I., Piazza, A., Rumi, L.S. & Polak De Fried, E. (1997) . Interleukin-1β levels in human embryo culture supernatants and their predictive value for pregnancy. Early Human Development, 48(1-2), 71-80.
http://dx.doi.org/10.1016/S0378-3782(96)01837-3
---------- CHICAGO ----------
Barañao, R.I., Piazza, A., Rumi, L.S., Polak De Fried, E. "Interleukin-1β levels in human embryo culture supernatants and their predictive value for pregnancy" . Early Human Development 48, no. 1-2 (1997) : 71-80.
http://dx.doi.org/10.1016/S0378-3782(96)01837-3
---------- MLA ----------
Barañao, R.I., Piazza, A., Rumi, L.S., Polak De Fried, E. "Interleukin-1β levels in human embryo culture supernatants and their predictive value for pregnancy" . Early Human Development, vol. 48, no. 1-2, 1997, pp. 71-80.
http://dx.doi.org/10.1016/S0378-3782(96)01837-3
---------- VANCOUVER ----------
Barañao, R.I., Piazza, A., Rumi, L.S., Polak De Fried, E. Interleukin-1β levels in human embryo culture supernatants and their predictive value for pregnancy. EARLY HUM. DEV. 1997;48(1-2):71-80.
http://dx.doi.org/10.1016/S0378-3782(96)01837-3