Artículo

Miranda Zanetti, J.; Greizerstein, E.; Camadro, E.; Poverene, M.; Echeverría, M.; Poggio, L.; Carrera, A. "Genomic relationships between hexaploid Helianthus resinosus and diploid Helianthus annuus (Asteraceae)" (2014) Plant Systematics and Evolution. 300(5):1071-1078
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Abstract:

Genus Helianthus comprises diploid and polyploid species. An autoallopolyploid origin has been proposed for hexaploid species but the genomic relationships remain unclear. Mitotic and meiotic studies in annual Helianthus annuus (2n = 2x = 34) and perennial Helianthus resinosus (2n = 6x = 102) as well as the F1 hybrids between both species were carried out. Chromosome counting confirmed the hybrid origin of the latter plants and their tetraploid condition. Bivalents in hybrids ranged from 12 to 28 (x̄ = 20.8). Univalents, trivalents and quadrivalents were also observed. Meiotic products comprised dyads, triads and normal tetrads and pollen grains were heterogeneous in size. These observations suggest the occurrence of 2n pollen in addition to the expected n. Genomic in situ hybridization (GISH) of total H. annuus DNA on H. resinosus chromosomes rendered weak but uniform signals; similar hybridization pattern was observed using three other annual species. Hybridization with H. annuus probe performed on root tip cells of F1 H. annuus × H. resinosus hybrids revealed 17 chromosomes with a strong hybridization signal. GISH in hybrid meiocytes distinguished chromosomes from parental species and revealed autosyndetic pairing of H. resinosus chromosomes, allosyndetic pairing in bivalents, trivalents and quadrivalents, and the presence of univalents derived from parents, H. annuus and H. resinosus. Results obtained from classical and molecular cytogenetics do not support H. annuus as a direct ancestor of H. resinosus. The occurrence of allosyndetic pairing and the relatively high fertility of the F1 hybrids point to the possibility that useful genes could be transferred from H. resinosus to cultivate sunflower, although the effective rate of recombination has not been evaluated. GISH method proved effective to recognize parental chromosomes in H. annuus × H. resinosus progeny. © 2013 Springer-Verlag Wien.

Registro:

Documento: Artículo
Título:Genomic relationships between hexaploid Helianthus resinosus and diploid Helianthus annuus (Asteraceae)
Autor:Miranda Zanetti, J.; Greizerstein, E.; Camadro, E.; Poverene, M.; Echeverría, M.; Poggio, L.; Carrera, A.
Filiación:Laboratorio de Biotecnología Vegetal CERZOS-CONICET/UNS, CCT Bahía Blanca Camino La Carrindanga km 7, 8000 Bahía Blanca, Argentina
Departamento de Agronomía, Universidad Nacional del Sur, San Andrés 800, 8000 Bahía Blanca, Argentina
Departamento de Ecología, Ciudad Universitaria, UBA Intendente Güiraldes 2160, C1428EGA Buenos Aires, Argentina
Universidad Nacional de Lomas de Zamora, Ruta 4, Km 2 Llavallol, 1846 Buenos Aires, Argentina
Estación Experimental Agropecuaria (EEA), Balcarce Instituto Nacional de Tecnología Agropecuaria (INTA), Buenos Aires, Argentina
Universidad Nacional de Mar del Plata (UNMdP), C.C. 256, 7620 Balcarce, Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Balcarce, Buenos Aires, Argentina
Palabras clave:2n pollen; Allosyndesis; GISH; Interspecific hybrid; Polyploidy; Sunflower; Asteraceae; Helianthus; Helianthus annuus; Helianthus resinosus
Año:2014
Volumen:300
Número:5
Página de inicio:1071
Página de fin:1078
DOI: http://dx.doi.org/10.1007/s00606-013-0945-0
Título revista:Plant Systematics and Evolution
Título revista abreviado:Plant Syst. Evol.
ISSN:03782697
CODEN:ESPFB
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03782697_v300_n5_p1071_MirandaZanetti

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Citas:

---------- APA ----------
Miranda Zanetti, J., Greizerstein, E., Camadro, E., Poverene, M., Echeverría, M., Poggio, L. & Carrera, A. (2014) . Genomic relationships between hexaploid Helianthus resinosus and diploid Helianthus annuus (Asteraceae). Plant Systematics and Evolution, 300(5), 1071-1078.
http://dx.doi.org/10.1007/s00606-013-0945-0
---------- CHICAGO ----------
Miranda Zanetti, J., Greizerstein, E., Camadro, E., Poverene, M., Echeverría, M., Poggio, L., et al. "Genomic relationships between hexaploid Helianthus resinosus and diploid Helianthus annuus (Asteraceae)" . Plant Systematics and Evolution 300, no. 5 (2014) : 1071-1078.
http://dx.doi.org/10.1007/s00606-013-0945-0
---------- MLA ----------
Miranda Zanetti, J., Greizerstein, E., Camadro, E., Poverene, M., Echeverría, M., Poggio, L., et al. "Genomic relationships between hexaploid Helianthus resinosus and diploid Helianthus annuus (Asteraceae)" . Plant Systematics and Evolution, vol. 300, no. 5, 2014, pp. 1071-1078.
http://dx.doi.org/10.1007/s00606-013-0945-0
---------- VANCOUVER ----------
Miranda Zanetti, J., Greizerstein, E., Camadro, E., Poverene, M., Echeverría, M., Poggio, L., et al. Genomic relationships between hexaploid Helianthus resinosus and diploid Helianthus annuus (Asteraceae). Plant Syst. Evol. 2014;300(5):1071-1078.
http://dx.doi.org/10.1007/s00606-013-0945-0