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Abstract:

To better understand Euglena gracilis gene expression under different stress conditions (Chromium, Streptomycin or darkness), we undertook a survey of the E. gracilis transcriptome by cDNA sequencing and microarray analysis. First, we constructed a non-normalized cDNA library from the E. gracilis UTEX strain and sequenced a total of 1000 cDNAs. Six hundred and ten of these ESTs were similar to either Plantae or Protistae genes (e-value < e- 10). Second, microarrays were built by spotting all the ESTs onto mirror slides. Microarray expression analysis indicated that 90 out of those 610 ESTs changed their expression level in response to different stress treatments (p < 0.05). In addition, we detected 10 ESTs that changed expression levels irrespective of the tested stress. These may be considered as part of a larger set of stress-related genes in E. gracilis. Finally, we identified 23 unknown ESTs (U-ESTs) following the expression profiles of these putative stress-related genes suggesting that they could be related to the cellular mechanism of stress response. © 2006 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress
Autor:dos Santos Ferreira, V.; Rocchetta, I.; Conforti, V.; Bench, S.; Feldman, R.; Levin, M.J.
Filiación:Laboratorio de Biología Molecular de la Enfermedad de Chagas (LaBMECh) INGEBI, National Research Council (CONICET), Centro de Genómica Aplicada (CeGA), Vuelta de Obligado 2490 2P, 1428 Buenos Aires, Argentina
Laboratorio de Biología Comparada, Protistas Departamento de Biodiversidad y Biología Experimental-DBBE (FCEyN), Universidad de Buenos Aires, Argentina
University of California Santa Cruz, Ocean Sciences Department, Earth and Marine Sciences Bldg., Santa Cruz, CA 95064, United States
SymBio Corporation, 1455 Adams Drive, Menlo Park, CA 94025, United States
lnstitut Cochin, D'epartement Maladies Infectieuses, INSERM U567, Paris F-75014, France
Palabras clave:cDNA library; Chromium; EST; Microarray; Streptomycin; chromium; complementary DNA; streptomycin; article; DNA microarray; DNA sequence; environmental stress; Euglena gracilis; expressed sequence tag; gene expression; nonhuman; nucleotide sequence; priority journal; Animals; Chromium; Darkness; DNA, Algal; DNA, Complementary; DNA, Protozoan; Euglena gracilis; Expressed Sequence Tags; Gene Expression Profiling; Gene Expression Regulation; Gene Library; Genes, Protozoan; Streptomycin; Euglena gracilis; Plantae
Año:2007
Volumen:389
Número:2
Página de inicio:136
Página de fin:145
DOI: http://dx.doi.org/10.1016/j.gene.2006.10.023
Título revista:Gene
Título revista abreviado:Gene
ISSN:03781119
CODEN:GENED
CAS:chromium, 16065-83-1, 7440-47-3; streptomycin, 57-92-1; Chromium, 7440-47-3; DNA, Algal; DNA, Complementary; DNA, Protozoan; Streptomycin, 57-92-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781119_v389_n2_p136_dosSantosFerreira

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Citas:

---------- APA ----------
dos Santos Ferreira, V., Rocchetta, I., Conforti, V., Bench, S., Feldman, R. & Levin, M.J. (2007) . Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress. Gene, 389(2), 136-145.
http://dx.doi.org/10.1016/j.gene.2006.10.023
---------- CHICAGO ----------
dos Santos Ferreira, V., Rocchetta, I., Conforti, V., Bench, S., Feldman, R., Levin, M.J. "Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress" . Gene 389, no. 2 (2007) : 136-145.
http://dx.doi.org/10.1016/j.gene.2006.10.023
---------- MLA ----------
dos Santos Ferreira, V., Rocchetta, I., Conforti, V., Bench, S., Feldman, R., Levin, M.J. "Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress" . Gene, vol. 389, no. 2, 2007, pp. 136-145.
http://dx.doi.org/10.1016/j.gene.2006.10.023
---------- VANCOUVER ----------
dos Santos Ferreira, V., Rocchetta, I., Conforti, V., Bench, S., Feldman, R., Levin, M.J. Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress. Gene. 2007;389(2):136-145.
http://dx.doi.org/10.1016/j.gene.2006.10.023