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Ayub, M.J.; Smulski, C.R.; Nyambega, B.; Bercovich, N.; Masiga, D.; Vazquez, M.P.; Aguilar, C.F.; Levin, M.J. "Protein-protein interaction map of the Trypanosoma cruzi ribosomal P protein complex" (2005) Gene. 357(2):129-136
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Abstract:

The large subunit of the eukaryotic ribosome possesses a long and protruding stalk formed by the ribosomal P proteins. Four out of five ribosomal P proteins of Trypanosoma cruzi, TcP0, TcP1α, TcP2α, and TcP2β had been previously characterized. Data mining of the T. cruzi genome data base allowed the identification of the fifth member of this protein group, a novel P1 protein, named P1β. To gain insight into the assembly of the stalk, a yeast two-hybrid based protein interaction map was generated. A parasite specific profile of interactions amongst the ribosomal P proteins of T. cruzi was evident. The TcP0 protein was able to interact with all both P1 and both P2 proteins. Moreover, the interactions between P2β with P1α as well as with P2α were detected, as well as the ability of TcP2β to homodimerize. A quantitative evaluation of the interactions established that the strongest interacting pair was TcP0-TcP1β. © 2005 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Protein-protein interaction map of the Trypanosoma cruzi ribosomal P protein complex
Autor:Ayub, M.J.; Smulski, C.R.; Nyambega, B.; Bercovich, N.; Masiga, D.; Vazquez, M.P.; Aguilar, C.F.; Levin, M.J.
Filiación:Laboratorio de Biología Molecular de la Enfermedad de Chagas (LaBMECH), Instituto de Investigaciones en Ingenieria Genetica Y Biologia Molecular (INGEBI/CONICET), Universidad de Buenos Aires, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Laboratorio de Biología Molecular Estructural, Facultad de Química, Bioquímica Y Farmacia, Universidad Nacional de San Luis, Ejército de los Andes 950, 5700 San Luis, Argentina
Molecular Biology and Biochemistry Department, International Centre of Insect Physiology and Ecology, P. O. Box 30772, 00100, Nairobi, Kenya
Palabras clave:Protein-protein interaction; Ribosomal P protein; Ribosome; Trypanosoma cruzi; Yeast two-hybrid; glycine cleavage system; article; complex formation; dimerization; evaluation; nonhuman; nucleotide sequence; priority journal; protein protein interaction; quantitative analysis; ribosome; Trypanosoma cruzi; Amino Acid Sequence; Animals; Dimerization; Molecular Sequence Data; Multiprotein Complexes; Peptide Mapping; Phosphoproteins; Protein Binding; Protozoan Proteins; Ribosomal Proteins; Trypanosoma cruzi; Two-Hybrid System Techniques; Eukaryota; Trypanosoma cruzi
Año:2005
Volumen:357
Número:2
Página de inicio:129
Página de fin:136
DOI: http://dx.doi.org/10.1016/j.gene.2005.06.006
Título revista:Gene
Título revista abreviado:Gene
ISSN:03781119
CODEN:GENED
CAS:Multiprotein Complexes; Phosphoproteins; Protozoan Proteins; ribosomal phosphoprotein P1; Ribosomal Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781119_v357_n2_p129_Ayub

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Citas:

---------- APA ----------
Ayub, M.J., Smulski, C.R., Nyambega, B., Bercovich, N., Masiga, D., Vazquez, M.P., Aguilar, C.F.,..., Levin, M.J. (2005) . Protein-protein interaction map of the Trypanosoma cruzi ribosomal P protein complex. Gene, 357(2), 129-136.
http://dx.doi.org/10.1016/j.gene.2005.06.006
---------- CHICAGO ----------
Ayub, M.J., Smulski, C.R., Nyambega, B., Bercovich, N., Masiga, D., Vazquez, M.P., et al. "Protein-protein interaction map of the Trypanosoma cruzi ribosomal P protein complex" . Gene 357, no. 2 (2005) : 129-136.
http://dx.doi.org/10.1016/j.gene.2005.06.006
---------- MLA ----------
Ayub, M.J., Smulski, C.R., Nyambega, B., Bercovich, N., Masiga, D., Vazquez, M.P., et al. "Protein-protein interaction map of the Trypanosoma cruzi ribosomal P protein complex" . Gene, vol. 357, no. 2, 2005, pp. 129-136.
http://dx.doi.org/10.1016/j.gene.2005.06.006
---------- VANCOUVER ----------
Ayub, M.J., Smulski, C.R., Nyambega, B., Bercovich, N., Masiga, D., Vazquez, M.P., et al. Protein-protein interaction map of the Trypanosoma cruzi ribosomal P protein complex. Gene. 2005;357(2):129-136.
http://dx.doi.org/10.1016/j.gene.2005.06.006