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Abstract:

A chromosomal region from Agrobacterium tumefaciens that complements exoC (pgm) mutations was cloned and sequenced. A cluster of three open reading frames (ORF1, ORF2 and ORF3) was identified. These genes are oriented in the same direction and are involved in the synthesis of glycogen and other polysaccharides. ORF1 encodes a 420-amino-acid (aa) protein with 55.9% homology to Escherichia coli GlgC (ADP-glucose synthetase, EC 2.7.7.27). ORF2 encodes a 480-aa protein with 42.2% homology to E. coli GlgA (glycogen synthase, EC 2.4.1.21). Based on Tn5 mutagenesis and protein homology, ORF3 was identified as the structural gene encoding phosphoglucomutase (Pgm, EC 2.7.5.1). ORF3 encodes a 542-aa protein with 52.6% homology to rabbit Pgm. There is no significant homology (less than 20%) to the Xanthomonas campestris XanA protein, which displays phosphomannomutase (Pmm) and Pgm activities [Koplin et al. J. Bacteriol 174 (1992) 191-199]. An A. tumefaciens pgm::Tn5 mutant retains Pmm activity. © 1994.

Registro:

Documento: Artículo
Título:A chromosomal cluster of genes encoding ADP-glucose synthetase, glycogen synthase and phosphoglucomutase in Agrobacterium tumefaciens
Autor:Uttaro, A.D.; Ugalde, R.A.
Filiación:Instituto de Investigaciones Bioquimicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, CONICET, 1405 Buenos Aires, Argentina
Palabras clave:cyclic β(l-2) glucan; exoC mutants; exopolysaccharides; glycogen; Nucleotide sequence; succinoglycan; exopolysaccharide; glucan; phosphomannomutase; succinoglycan; unclassified drug; amino acid sequence; article; enzyme activity; gene cluster; gene mapping; mutant; nonhuman; nucleotide sequence; priority journal; Rhizobium radiobacter; sequence homology; Agrobacterium tumefaciens; Amino Acid Sequence; Animal; Base Sequence; Chromosomes, Bacterial; Cloning, Molecular; DNA Transposable Elements; DNA, Bacterial; Genes, Bacterial; Genes, Structural, Bacterial; Molecular Sequence Data; Multigene Family; Mutation; Nucleotidyltransferases; Open Reading Frames; Phosphoglucomutase; Plasmids; Rabbits; Restriction Mapping; Sequence Homology, Amino Acid; Agrobacterium tumefaciens; Escherichia coli; Oryctolagus cuniculus; Xanthomonas campestris
Año:1994
Volumen:150
Número:1
Página de inicio:117
Página de fin:122
DOI: http://dx.doi.org/10.1016/0378-1119(94)90869-9
Título revista:Gene
Título revista abreviado:Gene
ISSN:03781119
CODEN:GENED
CAS:DNA Transposable Elements; DNA, Bacterial; glucose-1-phosphate adenylyltransferase, EC 2.7.7.27; Nucleotidyltransferases, EC 2.7.7; Phosphoglucomutase, EC 5.4.2.2; Plasmids
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781119_v150_n1_p117_Uttaro

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Citas:

---------- APA ----------
Uttaro, A.D. & Ugalde, R.A. (1994) . A chromosomal cluster of genes encoding ADP-glucose synthetase, glycogen synthase and phosphoglucomutase in Agrobacterium tumefaciens. Gene, 150(1), 117-122.
http://dx.doi.org/10.1016/0378-1119(94)90869-9
---------- CHICAGO ----------
Uttaro, A.D., Ugalde, R.A. "A chromosomal cluster of genes encoding ADP-glucose synthetase, glycogen synthase and phosphoglucomutase in Agrobacterium tumefaciens" . Gene 150, no. 1 (1994) : 117-122.
http://dx.doi.org/10.1016/0378-1119(94)90869-9
---------- MLA ----------
Uttaro, A.D., Ugalde, R.A. "A chromosomal cluster of genes encoding ADP-glucose synthetase, glycogen synthase and phosphoglucomutase in Agrobacterium tumefaciens" . Gene, vol. 150, no. 1, 1994, pp. 117-122.
http://dx.doi.org/10.1016/0378-1119(94)90869-9
---------- VANCOUVER ----------
Uttaro, A.D., Ugalde, R.A. A chromosomal cluster of genes encoding ADP-glucose synthetase, glycogen synthase and phosphoglucomutase in Agrobacterium tumefaciens. Gene. 1994;150(1):117-122.
http://dx.doi.org/10.1016/0378-1119(94)90869-9