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Abstract:

Cell-free extracts of epimastigotes of Trypanosoma cruzi contain tyrosine aminotransferase (TAT) and p-hydroxyphenyllactate dehydrogenase (pHPLDH). The TAT activity could be separated from aspartate aminotransferase (ASAT) by polyacrylamide gel electrophoresis or DEAE-cellulose chromatography; the latter procedure also allowed complete separation of pHPLDH. The subcellular localization of both T. cruzi enzymes, as determined by digitonin extraction, subcellular fractionation by differential centrifugation, and isopycnic ultracentrifugation in sucrose gradients, was mainly cytosolic, with low mitochondrial activities. © 1992.

Registro:

Documento: Artículo
Título:Presence and subcellular localization of tyrosine aminotransferase and p-hydroxyphenyllactate dehydrogenase in epimastigotes of Trypanosoma cruzi
Autor:Nowicki, C.; Montemartini, M.; Duschak, V.; Santomé, JosA.; Cazzulo, J.J.
Filiación:IQUIFIB, Buenos Aires, Argentina
Instituto de Investigaciones Bioquímicas Luis F. Leloir, Fundación Campomar - CONICET - Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:p-Hydroxyphenyl lactate dehydrogenase; Trypanosoma cruzi; Tyrosine aminotransferase; 4 hydroxyphenyllactic acid; 4-hydroxyphenyllactic acid; oxidoreductase; phenylpropionic acid derivative; tyrosine aminotransferase; aspartate aminotransferase; tyrosine aminotransferase; animal; article; cell cycle; cell fractionation; enzymology; isolation and purification; metabolism; Trypanosoma cruzi; ultrastructure; epimastigote; mitochondrion; nonhuman; priority journal; protein localization; trypanosoma cruzi; Animal; Cell Cycle; Oxidoreductases; Phenylpropionates; Subcellular Fractions; Support, Non-U.S. Gov't; Trypanosoma cruzi; Tyrosine Transaminase; Trypanosoma; Trypanosoma cruzi
Año:1992
Volumen:92
Número:2
Página de inicio:119
Página de fin:124
DOI: http://dx.doi.org/10.1016/0378-1097(92)90498-D
Título revista:FEMS Microbiology Letters
Título revista abreviado:FEMS Microbiol. Lett.
ISSN:03781097
CODEN:FMLED
CAS:4 hydroxyphenyllactic acid, 306-23-0; oxidoreductase, 9035-73-8, 9035-82-9, 9037-80-3, 9055-15-6; tyrosine aminotransferase, 9014-55-5; 4-hydroxyphenyllactic acid, 306-23-0; Oxidoreductases, EC 1.; Phenylpropionates; Tyrosine Transaminase, EC 2.6.1.5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v92_n2_p119_Nowicki

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Citas:

---------- APA ----------
Nowicki, C., Montemartini, M., Duschak, V., Santomé, JosA. & Cazzulo, J.J. (1992) . Presence and subcellular localization of tyrosine aminotransferase and p-hydroxyphenyllactate dehydrogenase in epimastigotes of Trypanosoma cruzi. FEMS Microbiology Letters, 92(2), 119-124.
http://dx.doi.org/10.1016/0378-1097(92)90498-D
---------- CHICAGO ----------
Nowicki, C., Montemartini, M., Duschak, V., Santomé, JosA., Cazzulo, J.J. "Presence and subcellular localization of tyrosine aminotransferase and p-hydroxyphenyllactate dehydrogenase in epimastigotes of Trypanosoma cruzi" . FEMS Microbiology Letters 92, no. 2 (1992) : 119-124.
http://dx.doi.org/10.1016/0378-1097(92)90498-D
---------- MLA ----------
Nowicki, C., Montemartini, M., Duschak, V., Santomé, JosA., Cazzulo, J.J. "Presence and subcellular localization of tyrosine aminotransferase and p-hydroxyphenyllactate dehydrogenase in epimastigotes of Trypanosoma cruzi" . FEMS Microbiology Letters, vol. 92, no. 2, 1992, pp. 119-124.
http://dx.doi.org/10.1016/0378-1097(92)90498-D
---------- VANCOUVER ----------
Nowicki, C., Montemartini, M., Duschak, V., Santomé, JosA., Cazzulo, J.J. Presence and subcellular localization of tyrosine aminotransferase and p-hydroxyphenyllactate dehydrogenase in epimastigotes of Trypanosoma cruzi. FEMS Microbiol. Lett. 1992;92(2):119-124.
http://dx.doi.org/10.1016/0378-1097(92)90498-D