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Abstract:

Plasmid pPG1 from Staphylococcus aureus coding for ampicillin (Apr), gentamicin (Gmr) and amikacin (Akr) resistance was transformed into Escherichia coli. Transformation efficiency was about 2 × 103 transformants/μg of plasmid DNA. The plasmids present in the E. coli transformants were identical to pPG1 according to their restriction patterns. The copy number of pPG1 was estimated to be at least 20-times less in E. coli than in S. aureus. The minimal inhibitory concentrations (MICs) for Ap and Gm were lower in E. coli than in S. aureus. However, the MIC for Ak was higher in E. coli transformants than in S. aureus. pPG1 was maintained in the E. coli transformants for at least 80 generations at 37°C without antibiotic selection pressure. © 1991.

Registro:

Documento: Artículo
Título:A staphylococcal plasmid that replicates and expresses ampicillin, gentamicin and amikacin resistance in Escherichia coli
Autor:Gadaleta, P.; Kaufman, S.; Martini, P.; Zorzópulos, J.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Hospital Juan A. Fernandez, Buenos Aires, Argentina
BioSidus S.A., Buenos Aires, Argentina
Palabras clave:Antibiotic resistance; Hetero specific gene exchange; Staphylococcus plasmid; amikacin; gentamicin; amikacin; ampicillin; chloramphenicol; gentamicin; tetracycline; antibiotic resistance; article; bacterium transformation; Escherichia coli; genetics; penicillin resistance; plasmid; Staphylococcus aureus; dna hybridization; escherichia coli; nonhuman; plasmid; priority journal; southern blotting; staphylococcus; Amikacin; Ampicillin Resistance; Drug Resistance, Microbial; Escherichia coli; Gentamicins; Plasmids; Staphylococcus aureus; Support, Non-U.S. Gov't; Transformation, Bacterial; Escherichia coli; Staphylococcus; Staphylococcus aureus
Año:1991
Volumen:80
Número:2-3
Página de inicio:147
Página de fin:150
DOI: http://dx.doi.org/10.1111/j.1574-6968.1991.tb04651.x
Título revista:FEMS Microbiology Letters
Título revista abreviado:FEMS Microbiol. Lett.
ISSN:03781097
CODEN:FMLED
CAS:amikacin, 37517-28-5, 39831-55-5; gentamicin, 1392-48-9, 1403-66-3, 1405-41-0; Amikacin, 37517-28-5; Gentamicins; Plasmids
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v80_n2-3_p147_Gadaleta

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Citas:

---------- APA ----------
Gadaleta, P., Kaufman, S., Martini, P. & Zorzópulos, J. (1991) . A staphylococcal plasmid that replicates and expresses ampicillin, gentamicin and amikacin resistance in Escherichia coli. FEMS Microbiology Letters, 80(2-3), 147-150.
http://dx.doi.org/10.1111/j.1574-6968.1991.tb04651.x
---------- CHICAGO ----------
Gadaleta, P., Kaufman, S., Martini, P., Zorzópulos, J. "A staphylococcal plasmid that replicates and expresses ampicillin, gentamicin and amikacin resistance in Escherichia coli" . FEMS Microbiology Letters 80, no. 2-3 (1991) : 147-150.
http://dx.doi.org/10.1111/j.1574-6968.1991.tb04651.x
---------- MLA ----------
Gadaleta, P., Kaufman, S., Martini, P., Zorzópulos, J. "A staphylococcal plasmid that replicates and expresses ampicillin, gentamicin and amikacin resistance in Escherichia coli" . FEMS Microbiology Letters, vol. 80, no. 2-3, 1991, pp. 147-150.
http://dx.doi.org/10.1111/j.1574-6968.1991.tb04651.x
---------- VANCOUVER ----------
Gadaleta, P., Kaufman, S., Martini, P., Zorzópulos, J. A staphylococcal plasmid that replicates and expresses ampicillin, gentamicin and amikacin resistance in Escherichia coli. FEMS Microbiol. Lett. 1991;80(2-3):147-150.
http://dx.doi.org/10.1111/j.1574-6968.1991.tb04651.x