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Abstract:

Epinastigotes of different stocks of Trypanosoma cruzi contain levels of proteinase activity on azocasein; amastigotes and trypmastigotes contain 10-fold lower levels of this proteolytic activity, which seems, therefore, to be developmentally regulated. The proteinase could be detected as a broad band, centered at about 60 kDa, which in some resolved into two close bands, in (a) SDS-polyacrylamide gels containing fibrinogen, and (b) Western blots probed with polyclonal rabbit antiserum prepared against purified cystein proteinase. No proteinase activity was observed at molecular weights lower than 55 kDa. The results show that the enzyme preciously purified is teh major cysteine proteinase present in epimastigotes of all stocks of T. cruzi tested. © 1990.

Registro:

Documento: Artículo
Título:A major cysteine proteinase is developmentally regulated in Trypanosoma cruzi
Autor:Campetella, O.; Martínez, J.; Cazzulo, J.J.
Filiación:Instituto de Investigaciones Bioquímicas Fundaciòn Campomar, Facultad de Ciencia Exactas y Naturales, Universidad de Buenos Airesd-CONICET, Buenos Aires, Argentina
Palabras clave:Cysteine proteinases; Polyacrylamide gels; Trypanosoma cruzi; Western blots; cysteine proteinase; cysteine proteinase; article; filaria; nonhuman; priority journal; protozoon; trypanosoma; animal; enzymology; growth, development and aging; isolation and purification; metabolism; molecular weight; polyacrylamide gel electrophoresis; Trypanosoma cruzi; Western blotting; Filaria; Oryctolagus cuniculus; Protozoa; Trypanosoma; Trypanosoma cruzi; Animal; Blotting, Western; Cysteine Endopeptidases; Electrophoresis, Polyacrylamide Gel; Molecular Weight; Support, Non-U.S. Gov't; Trypanosoma cruzi
Año:1990
Volumen:67
Número:1-2
Página de inicio:145
Página de fin:149
DOI: http://dx.doi.org/10.1016/0378-1097(90)90184-R
Título revista:FEMS Microbiology Letters
Título revista abreviado:FEMS Microbiol. Lett.
ISSN:03781097
CODEN:FMLED
CAS:cysteine proteinase, 37353-41-6; Cysteine Endopeptidases, EC 3.4.22
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v67_n1-2_p145_Campetella

Referencias:

  • Cazzulo, (1984) Comp. Biochem. Physiol., 79 B, pp. 309-320
  • Bontempi, Franke de Cazzulo, Ruiz, Cazzulo, (1984) Comp. Biochem. Physiol., 77 B, pp. 599-604
  • Cazzulo, Couso, Raimondi, Wernstedt, Hellman, (1989) Mol. Biochem. Parasitol., 33, pp. 33-42
  • Bontempi, Martinez, Cazzulo, (1989) Mol. Biochem. Parasitol., 33, pp. 43-48
  • Martinez, Silberstein, Cazzulo, (1989) Anales Asoc. Quim. Argentina, 77, pp. 113-117
  • Conteras, Sales, Thomas, Morel, Goldenberg, (1985) Mol. Biochem. Parasitol., 16, pp. 315-327
  • Cazzulo, Franke de Cazzulo, Engel, Cannata, (1985) Mol. Biochem. Parasitol., 16, pp. 329-343
  • Andrews, Colli, (1982) J. Protozool., 29, pp. 264-269
  • Lonsdale-Eccles, Mpimbaza, (1986) Eur. J. Biochem., 155, pp. 469-473
  • Laemmli, (1970) Nature, 227, pp. 680-685
  • Lowry, Rosenbrough, Farr, Randall, (1951) J. Biol. Chem., 193, pp. 265-275
  • Pamer, So, Davis, (1989) Mol. Biochem. Parasitol., 33, pp. 27-32

Citas:

---------- APA ----------
Campetella, O., Martínez, J. & Cazzulo, J.J. (1990) . A major cysteine proteinase is developmentally regulated in Trypanosoma cruzi. FEMS Microbiology Letters, 67(1-2), 145-149.
http://dx.doi.org/10.1016/0378-1097(90)90184-R
---------- CHICAGO ----------
Campetella, O., Martínez, J., Cazzulo, J.J. "A major cysteine proteinase is developmentally regulated in Trypanosoma cruzi" . FEMS Microbiology Letters 67, no. 1-2 (1990) : 145-149.
http://dx.doi.org/10.1016/0378-1097(90)90184-R
---------- MLA ----------
Campetella, O., Martínez, J., Cazzulo, J.J. "A major cysteine proteinase is developmentally regulated in Trypanosoma cruzi" . FEMS Microbiology Letters, vol. 67, no. 1-2, 1990, pp. 145-149.
http://dx.doi.org/10.1016/0378-1097(90)90184-R
---------- VANCOUVER ----------
Campetella, O., Martínez, J., Cazzulo, J.J. A major cysteine proteinase is developmentally regulated in Trypanosoma cruzi. FEMS Microbiol. Lett. 1990;67(1-2):145-149.
http://dx.doi.org/10.1016/0378-1097(90)90184-R