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Abstract:

To determine whether the stationary sigma factor, σS, influences polyhydroxyalkanoate metabolism in Pseudomonas putida KT2440, an rpoS-negative mutant was constructed to evaluate polyhydroxyalkanoate accumulation and expression of a translational fusion to the promoter region of the genes that code for polyhydroxyalkanoate synthase 1 (phaC1) and polyhydroxyalkanoate depolymerase (phaZ). By comparison with the wild-type, the rpoS mutant showed a higher polyhydroxyalkanoate degradation rate and increased expression of the translational fusion during the stationary growth phase. These results suggest that σS might control the genes involved in polyhydroxyalkanoate metabolism, possibly in an indirect manner. In addition, survival and oxidative stress assays performed under polyhydroxyalkanoate- and nonpolyhydroxyalkanoate- accumulating conditions demonstrated that the accumulated polyhydroxyalkanoate increased the survival and stress tolerance of the rpoS mutant. According to this, polyhydroxyalkanoate accumulation would help cells to overcome the adverse conditions encountered during the stationary phase in the strain that lacks RpoS. © 2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:The alternative sigma factor, σS, affects polyhydroxyalkanoate metabolism in Pseudomonas putida
Autor:Raiger-Iustman, L.J.; Ruiz, J.A.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, 1428. Buenos Aires, Argentina
Palabras clave:Polyhydroxyalkanoate; Pseudomonas putida; Regulation; RpoS; Stress resistance; bacterial enzyme; polyhydroxyalkanoate depolymerase; polyhydroxyalkanoate synthase; polyhydroxyalkanoate synthase 1; polyhydroxyalkanoic acid; sigma factor; sigma factor RpoS; article; bacterial metabolism; bacterial strain; bacterial survival; controlled study; degradation; gene inactivation; nonhuman; oxidative stress; priority journal; promoter region; protein expression; Pseudomonas putida; Bacterial Proteins; Cloning, Molecular; Gene Expression Regulation, Bacterial; Gene Silencing; Hydrogen Peroxide; Microbial Viability; Oxidative Stress; Plasmids; Polyhydroxyalkanoates; Promoter Regions (Genetics); Pseudomonas putida; Recombinant Fusion Proteins; Sigma Factor; Time; Pseudomonas putida
Año:2008
Volumen:284
Número:2
Página de inicio:218
Página de fin:224
DOI: http://dx.doi.org/10.1111/j.1574-6968.2008.01203.x
Título revista:FEMS Microbiology Letters
Título revista abreviado:FEMS Microbiol. Lett.
ISSN:03781097
CODEN:FMLED
CAS:Bacterial Proteins; Hydrogen Peroxide, 7722-84-1; Polyhydroxyalkanoates; Recombinant Fusion Proteins; sigma factor KatF protein, Bacteria; Sigma Factor
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_03781097_v284_n2_p218_RaigerIustman.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v284_n2_p218_RaigerIustman

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Citas:

---------- APA ----------
Raiger-Iustman, L.J. & Ruiz, J.A. (2008) . The alternative sigma factor, σS, affects polyhydroxyalkanoate metabolism in Pseudomonas putida. FEMS Microbiology Letters, 284(2), 218-224.
http://dx.doi.org/10.1111/j.1574-6968.2008.01203.x
---------- CHICAGO ----------
Raiger-Iustman, L.J., Ruiz, J.A. "The alternative sigma factor, σS, affects polyhydroxyalkanoate metabolism in Pseudomonas putida" . FEMS Microbiology Letters 284, no. 2 (2008) : 218-224.
http://dx.doi.org/10.1111/j.1574-6968.2008.01203.x
---------- MLA ----------
Raiger-Iustman, L.J., Ruiz, J.A. "The alternative sigma factor, σS, affects polyhydroxyalkanoate metabolism in Pseudomonas putida" . FEMS Microbiology Letters, vol. 284, no. 2, 2008, pp. 218-224.
http://dx.doi.org/10.1111/j.1574-6968.2008.01203.x
---------- VANCOUVER ----------
Raiger-Iustman, L.J., Ruiz, J.A. The alternative sigma factor, σS, affects polyhydroxyalkanoate metabolism in Pseudomonas putida. FEMS Microbiol. Lett. 2008;284(2):218-224.
http://dx.doi.org/10.1111/j.1574-6968.2008.01203.x