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Abstract:

arcA codes for a central regulator in Escherichia coli that responds to redox conditions of growth. Mutations in this gene, originally named dye, confer sensitivity to toluidine blue and other redox dyes. However, the molecular basis for the dye-sensitive phenotype has not been elucidated. In this work, we show that toluidine blue redirects electrons to O2 and causes an increase in the generation of reactive O2 species (ROS). We also demonstrate that synthesis of poly (3-hydroxybutyrate) suppresses the Dye phenotype in E. coli recombinants, as the capacity to synthesize the polymer reduces sensitivity to toluidine blue, O2 consumption and ROS production levels. © 2006 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:Dye( arc)mutants: Insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants
Autor:Ruiz, J.A.; Fernández, R.O.; Nikel, P.I.; Méndez, B.S.; Pettinari, M.J.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Radiobiología, Comisión Nacional de Energía Atómica, San Martín, Argentina
Instituto de Investigaciones Biotecnológicas, Universidad Nacional de General San Martín, San Martín, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, 1428. Buenos Aires, Argentina
Palabras clave:arcA; O2 consumption; PHB; ROS; Toluidine blue; poly(3 hydroxybutyric acid); tolonium chloride; aerobic metabolism; article; controlled study; Escherichia coli; gene mutation; genetic code; molecular dynamics; nonhuman; oxidation reduction reaction; oxygen consumption; phenotype; polymerization; priority journal; Bacterial Outer Membrane Proteins; Escherichia coli; Escherichia coli Proteins; Hydroxybutyrates; Mutation; Oxygen Consumption; Phenotype; Polyesters; Reactive Oxygen Species; Recombination, Genetic; Repressor Proteins; Arca; Escherichia coli
Año:2006
Volumen:258
Número:1
Página de inicio:55
Página de fin:60
DOI: http://dx.doi.org/10.1111/j.1574-6968.2006.00196.x
Título revista:FEMS Microbiology Letters
Título revista abreviado:FEMS Microbiol. Lett.
ISSN:03781097
CODEN:FMLED
CAS:poly(3 hydroxybutyric acid), 26063-00-3; tolonium chloride, 92-31-9; arcA protein, E coli; Bacterial Outer Membrane Proteins; Escherichia coli Proteins; Hydroxybutyrates; poly-beta-hydroxybutyrate, 26063-00-3; Polyesters; Reactive Oxygen Species; Repressor Proteins
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_03781097_v258_n1_p55_Ruiz.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v258_n1_p55_Ruiz

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Citas:

---------- APA ----------
Ruiz, J.A., Fernández, R.O., Nikel, P.I., Méndez, B.S. & Pettinari, M.J. (2006) . Dye( arc)mutants: Insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants. FEMS Microbiology Letters, 258(1), 55-60.
http://dx.doi.org/10.1111/j.1574-6968.2006.00196.x
---------- CHICAGO ----------
Ruiz, J.A., Fernández, R.O., Nikel, P.I., Méndez, B.S., Pettinari, M.J. "Dye( arc)mutants: Insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants" . FEMS Microbiology Letters 258, no. 1 (2006) : 55-60.
http://dx.doi.org/10.1111/j.1574-6968.2006.00196.x
---------- MLA ----------
Ruiz, J.A., Fernández, R.O., Nikel, P.I., Méndez, B.S., Pettinari, M.J. "Dye( arc)mutants: Insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants" . FEMS Microbiology Letters, vol. 258, no. 1, 2006, pp. 55-60.
http://dx.doi.org/10.1111/j.1574-6968.2006.00196.x
---------- VANCOUVER ----------
Ruiz, J.A., Fernández, R.O., Nikel, P.I., Méndez, B.S., Pettinari, M.J. Dye( arc)mutants: Insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants. FEMS Microbiol. Lett. 2006;258(1):55-60.
http://dx.doi.org/10.1111/j.1574-6968.2006.00196.x