Abstract:
arcA codes for a central regulator in Escherichia coli that responds to redox conditions of growth. Mutations in this gene, originally named dye, confer sensitivity to toluidine blue and other redox dyes. However, the molecular basis for the dye-sensitive phenotype has not been elucidated. In this work, we show that toluidine blue redirects electrons to O2 and causes an increase in the generation of reactive O2 species (ROS). We also demonstrate that synthesis of poly (3-hydroxybutyrate) suppresses the Dye phenotype in E. coli recombinants, as the capacity to synthesize the polymer reduces sensitivity to toluidine blue, O2 consumption and ROS production levels. © 2006 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Registro:
Documento: |
Artículo
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Título: | Dye( arc)mutants: Insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants |
Autor: | Ruiz, J.A.; Fernández, R.O.; Nikel, P.I.; Méndez, B.S.; Pettinari, M.J. |
Filiación: | Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina Departamento de Radiobiología, Comisión Nacional de Energía Atómica, San Martín, Argentina Instituto de Investigaciones Biotecnológicas, Universidad Nacional de General San Martín, San Martín, Argentina Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, 1428. Buenos Aires, Argentina
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Palabras clave: | arcA; O2 consumption; PHB; ROS; Toluidine blue; poly(3 hydroxybutyric acid); tolonium chloride; aerobic metabolism; article; controlled study; Escherichia coli; gene mutation; genetic code; molecular dynamics; nonhuman; oxidation reduction reaction; oxygen consumption; phenotype; polymerization; priority journal; Bacterial Outer Membrane Proteins; Escherichia coli; Escherichia coli Proteins; Hydroxybutyrates; Mutation; Oxygen Consumption; Phenotype; Polyesters; Reactive Oxygen Species; Recombination, Genetic; Repressor Proteins; Arca; Escherichia coli |
Año: | 2006
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Volumen: | 258
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Número: | 1
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Página de inicio: | 55
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Página de fin: | 60
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DOI: |
http://dx.doi.org/10.1111/j.1574-6968.2006.00196.x |
Título revista: | FEMS Microbiology Letters
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Título revista abreviado: | FEMS Microbiol. Lett.
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ISSN: | 03781097
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CODEN: | FMLED
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CAS: | poly(3 hydroxybutyric acid), 26063-00-3; tolonium chloride, 92-31-9; arcA protein, E coli; Bacterial Outer Membrane Proteins; Escherichia coli Proteins; Hydroxybutyrates; poly-beta-hydroxybutyrate, 26063-00-3; Polyesters; Reactive Oxygen Species; Repressor Proteins
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PDF: | https://bibliotecadigital.exactas.uba.ar/download/paper/paper_03781097_v258_n1_p55_Ruiz.pdf |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v258_n1_p55_Ruiz |
Referencias:
- Ballesteros M, Fredriksson, A., Henriksson, J., Nystrom, T., (2001) Bacterial Senescence, 20, pp. 5280-5289. , protein oxidation in non-proliferating cells is dictated by the accuracy of the ribosomes EMBO J
- Balzan R, Sapienza, K., Galea, D.R., Vassallo, N., Frey, H., Bannister, W.H., (2004) Aspirin Commits Yeast Cells to Apoptosis Depending on Carbon Source, 150, pp. 109-115. , Microbiology
- Buxton R, Drury, L., (1983) Cloning and Insertional Inactivation of the Dye (Sfra) Gene, Mutation of Which Affects Sex Factor F Expression and Dye Sensitivity of Escherichia Coli K-12, 154, pp. 1309-13014. , J Bacteriol
- Cabiscol E, Tamarit, J., Ros, J., (2000) Oxidative Stress in Bacteria and Protein Damage by Reactive Oxygen Species, 3, pp. 3-8. , Int Microbiol
- Iuchi S, Lin, E.C., (1988) Arca (Dye), a Global Regulatory Gene in Escherichia Coli Mediating Repression of Enzymes in Aerobic Pathways, 85, pp. 1888-1892. , Proc Natl Acad Sci USA
- Lowry O, H., Rosebrough, N.J., Far, A.L., Randall, R.J., (1951) Protein Measure with the Folin Phenol Reagent, 193, pp. 265-267. , J Biol Chem
- Lynch A, Lin, E., Responses to molecular oxygen (1996) Escherichia Coli and Salmonella: Cellular and Molecular Biology*Neidhardt F.C. *curtis III. R. *ingraham J.L. *lin E.C.C. *low K.B. *magasanik B. *reznikoff W.S. *riley M. *schaechter M. *umbarger H.E., pp. 1526-1538. , American Society for Microbiology Washington, DC eds), pp
- Madison L, L., Huisman, G.W., (1999) Metabolic Engineering of poly(3-hydroxyalkanoates), 63, pp. 21-53. , from DNA to plastic Microbiol Mol Biol Rev
- Nikel P, I., Pettinari, M.J., Méndez, B.S., Galvagno, M.A., (2005) Statistical Optimization of a Culture Medium for Biomass and poly(3-hydroxybutyrate) Production by a Recombinant Escherichia Coli Strain Using Agroindustrial By-products, 8, pp. 243-250. , Int Microbiol
- Nystrom T, Larsson, C., Gustafsson, L., (1996) Bacterial Defense Against Aging, 15, pp. 3219-3218. , role of the Escherichia coli ArcA regulator in gene expression, readjusted energy flux and survival during stasis EMBO J
- Okon Y, Itzigsohn, R., (1992) Polyhydroxybutyrate Metabolism in Azospirillum Brasilense and the Ecological Role of PHB in the Rhizosphere, 103, pp. 131-141. , FEMS Microbiol Rev
- Overath P, Schairer, H.U., Stoffel, W., (1970) Correlation of in Vivo and in Vitro Phase Transitions of Membrane Lipids in Escherichia Coli, 67, pp. 606-612. , Proc Natl Acad Sci USA
- Patschkowski T, Bates, D.N., Kiley, P.J., Mechanisms for sensing and responding to oxygen deprivation (2000) Bacterial Stress Responses*Storz G. *hengge-Aronis R., pp. 61-78. , ASM Press Washington, DC eds), pp
- Pettinari M, J., Vazquez, G.J., Silberschmidt, D., Rehm, B., Steinbuchel, A., Mendez, B.S., (2001) Poly(3-hydroxybutyrate) Synthesis Genes in Azotobacter Sp. Strain FA8, 67, pp. 5331-5334. , Appl Environ Microbiol
- Roeder W, Somerville, R., (1979) Cloning the Trpr Gene, 176, pp. 361-368. , Mol Gen Genet
- Ruiz J, A., Lopez, N.I., Fernandez, R.O., Mendez, B.S., (2001) Polyhydroxyalkanoate Degradation Is Associated with Nucleotide Accumulation and Enhances Stress Resistance and Survival of Pseudomonas Oleovorans in Natural Water Microcosms, 67, pp. 225-230. , Appl Environ Microbiol
- Sambrook J, Fritsch, E.F., Maniatis, T., (1989) Molecular Cloning: A Laboratory Manual, , Cold Spring Laboratory Cold Spring Harbor, NY
- Somerville G, A., Beres, S.B., Fitzgerald, J.R., De Leo, F.R., Cole, R.L., Hoff, J.S., Musser, J.M., (2002) In Vitro Serial Passage of Staphylococcus Aureus, 184, pp. 1430-1437. , changes in physiology, virulence factor production, and agr nucleotide sequence J Bacteriol
- Wang R, F., Kushner, S.R., (1991) Construction of Versatile Low-copy-number Vectors for Cloning, Sequencing and Gene Expression in Escherichia Coli, 100, pp. 195-199. , Gene
Citas:
---------- APA ----------
Ruiz, J.A., Fernández, R.O., Nikel, P.I., Méndez, B.S. & Pettinari, M.J.
(2006)
. Dye( arc)mutants: Insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants. FEMS Microbiology Letters, 258(1), 55-60.
http://dx.doi.org/10.1111/j.1574-6968.2006.00196.x---------- CHICAGO ----------
Ruiz, J.A., Fernández, R.O., Nikel, P.I., Méndez, B.S., Pettinari, M.J.
"Dye( arc)mutants: Insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants"
. FEMS Microbiology Letters 258, no. 1
(2006) : 55-60.
http://dx.doi.org/10.1111/j.1574-6968.2006.00196.x---------- MLA ----------
Ruiz, J.A., Fernández, R.O., Nikel, P.I., Méndez, B.S., Pettinari, M.J.
"Dye( arc)mutants: Insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants"
. FEMS Microbiology Letters, vol. 258, no. 1, 2006, pp. 55-60.
http://dx.doi.org/10.1111/j.1574-6968.2006.00196.x---------- VANCOUVER ----------
Ruiz, J.A., Fernández, R.O., Nikel, P.I., Méndez, B.S., Pettinari, M.J. Dye( arc)mutants: Insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants. FEMS Microbiol. Lett. 2006;258(1):55-60.
http://dx.doi.org/10.1111/j.1574-6968.2006.00196.x