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Abstract:

Malachite green (MG) is a triphenylmethane dye used as a fungicide but also possesses a high toxicity to mammalian cells. The toxicity of MG to Fomes sclerodermeus and Phanerochaete chrysosporium was assessed. P. chrysosporium was highly sensitive to the dye and it was unable to grow on solid media containing 64 μM of MG, lower concentrations caused a delay in growth. The radial growth of F. sclerodermeus was not affected at this concentration and up to 128 μM. In liquid media both fungi were more sensitive. F. sclerodermeus not only was able to grow in the presence of high concentrations of MG, but also it was able to decolorize and detoxify the dye. MG treated with supernatants containing high laccase activity in the presence or absence of 1-hydroxybenzotriazole (1-HBT) gave a colorless product (DMG) that was not toxic to P. chrysosporium and other white rot fungi tested. On the basis of the data of maximal absorbance, it is probable that the mechanism involved in the modification of the dye was different if 1-HBT was added to the reaction. © 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Modification of malachite green by Fomes sclerodermeus and reduction of toxicity to Phanerochaete chrysosporium
Autor:Papinutti, V.L.; Forchiassin, F.
Filiación:Depto. de Biodiversidad y Biol. Exp., Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, BA, Argentina
Palabras clave:Fomes sclerodermeus; Laccase; Ligninase; Phanerochaete chrysosporium; White rot fungus; 1 hydroxybenzotriazole; benzotriazole derivative; dye; laccase; malachite green; unclassified drug; article; chemical modification; concentration response; controlled study; culture medium; cytotoxicity; decolorization; detoxification; enzyme activity; Fomes sclerodermeus; fungicidal activity; fungus; fungus growth; nonhuman; Phanerochaete; priority journal; reaction analysis; reduction; supernatant; Chrysosporium; Fomes; Fungi; Mammalia; Phanerochaete chrysosporium; Siproeta stelenes
Año:2004
Volumen:231
Número:2
Página de inicio:205
Página de fin:209
DOI: http://dx.doi.org/10.1016/S0378-1097(03)00957-1
Título revista:FEMS Microbiology Letters
Título revista abreviado:FEMS Microbiol. Lett.
ISSN:03781097
CODEN:FMLED
CAS:laccase, 80498-15-3; malachite green, 569-64-2
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_03781097_v231_n2_p205_Papinutti.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v231_n2_p205_Papinutti

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Citas:

---------- APA ----------
Papinutti, V.L. & Forchiassin, F. (2004) . Modification of malachite green by Fomes sclerodermeus and reduction of toxicity to Phanerochaete chrysosporium. FEMS Microbiology Letters, 231(2), 205-209.
http://dx.doi.org/10.1016/S0378-1097(03)00957-1
---------- CHICAGO ----------
Papinutti, V.L., Forchiassin, F. "Modification of malachite green by Fomes sclerodermeus and reduction of toxicity to Phanerochaete chrysosporium" . FEMS Microbiology Letters 231, no. 2 (2004) : 205-209.
http://dx.doi.org/10.1016/S0378-1097(03)00957-1
---------- MLA ----------
Papinutti, V.L., Forchiassin, F. "Modification of malachite green by Fomes sclerodermeus and reduction of toxicity to Phanerochaete chrysosporium" . FEMS Microbiology Letters, vol. 231, no. 2, 2004, pp. 205-209.
http://dx.doi.org/10.1016/S0378-1097(03)00957-1
---------- VANCOUVER ----------
Papinutti, V.L., Forchiassin, F. Modification of malachite green by Fomes sclerodermeus and reduction of toxicity to Phanerochaete chrysosporium. FEMS Microbiol. Lett. 2004;231(2):205-209.
http://dx.doi.org/10.1016/S0378-1097(03)00957-1