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The pathways for putrescine biosynthesis and the effects of polyamine biosynthesis inhibitors on the germination and hyphal development of Gigaspora rosea spores were investigated. Incubation of spores with different radioactive substrates demonstrated that both arginine and ornithine decarboxylase pathways participate in putrescine biosynthesis in G. rosea. Spermidine and spermine were the most abundant polyamines in this fungus. The putrescine biosynthesis inhibitors α-difluoromethylarginine and α-difluoromethylornithine, as well as the spermidine synthase inhibitor cyclohexylamine, slightly decreased polyamine levels. However, only the latter interfered with spore germination. The consequences of the use of putrescine biosynthesis inhibitors for the control of plant pathogenic fungi on the viability of G. rosea spores in soil are discussed. © 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.


Documento: Artículo
Título:Ornithine and arginine decarboxylase activities and effect of some polyamine biosynthesis inhibitors on Gigaspora rosea germinating spores
Autor:Sannazzaro, A.I.; Álvarez, C.L.; Menéndez, A.B.; Pieckenstain, F.L.; Albertó, E.O.; Ruiz, O.A.
Filiación:Inst. de Invest. Biotecnologicas, Inst. Tecn. de Chascomús, Consejo Nac. de Invest. Cie. y Tec., Casilla de Correo 164, 7130 Chascomús, Buenos Aires, Argentina
Depto. de Biodiversidad y Biol. Exp., Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Arbuscular mycorrhizal fungus; Arginine decarboxylase; Cyclohexylamine; Ornithine decarboxylase; α-Difluoromethylarginine; α-Difluoromethylornithine; alpha difluoromethylarginine; arginine decarboxylase; cycloheximide; eflornithine; enzyme inhibitor; ornithine decarboxylase; polyamine; putrescine; radioactive material; spermidine; spermidine synthase; spermine; article; biosynthesis; cell viability; controlled study; enzyme activity; fungus; fungus hyphae; fungus spore; germination; Gigaspora rosea; microbial pest control; nonhuman; plant disease; polyamine synthesis; priority journal; quantitative analysis; Fungi; Gigaspora; Gigaspora rosea
Página de inicio:115
Página de fin:121
Título revista:FEMS Microbiology Letters
Título revista abreviado:FEMS Microbiol. Lett.
CAS:alpha difluoromethylarginine, 69955-43-7; arginine decarboxylase, 9024-77-5; cycloheximide, 642-81-9, 66-81-9; eflornithine, 67037-37-0, 70052-12-9; ornithine decarboxylase, 9024-60-6; putrescine, 110-60-1, 333-93-7; spermidine, 124-20-9, 334-50-9; spermidine synthase, 37277-82-0, 67016-02-8; spermine, 306-67-2, 71-44-3


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---------- APA ----------
Sannazzaro, A.I., Álvarez, C.L., Menéndez, A.B., Pieckenstain, F.L., Albertó, E.O. & Ruiz, O.A. (2004) . Ornithine and arginine decarboxylase activities and effect of some polyamine biosynthesis inhibitors on Gigaspora rosea germinating spores. FEMS Microbiology Letters, 230(1), 115-121.
---------- CHICAGO ----------
Sannazzaro, A.I., Álvarez, C.L., Menéndez, A.B., Pieckenstain, F.L., Albertó, E.O., Ruiz, O.A. "Ornithine and arginine decarboxylase activities and effect of some polyamine biosynthesis inhibitors on Gigaspora rosea germinating spores" . FEMS Microbiology Letters 230, no. 1 (2004) : 115-121.
---------- MLA ----------
Sannazzaro, A.I., Álvarez, C.L., Menéndez, A.B., Pieckenstain, F.L., Albertó, E.O., Ruiz, O.A. "Ornithine and arginine decarboxylase activities and effect of some polyamine biosynthesis inhibitors on Gigaspora rosea germinating spores" . FEMS Microbiology Letters, vol. 230, no. 1, 2004, pp. 115-121.
---------- VANCOUVER ----------
Sannazzaro, A.I., Álvarez, C.L., Menéndez, A.B., Pieckenstain, F.L., Albertó, E.O., Ruiz, O.A. Ornithine and arginine decarboxylase activities and effect of some polyamine biosynthesis inhibitors on Gigaspora rosea germinating spores. FEMS Microbiol. Lett. 2004;230(1):115-121.