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Abstract:

The first specific precursor of porphyrin biosynthesis is δ-aminolevulinic acid. δ-Aminolevulinic acid enters Saccharomyces cerevisiae cells through the γ-aminobutyric acid specific permease Uga4p. It was described that this permease is inducible by γ-aminobutyric acid and its regulation involves several specific and pleiotropic transcriptional factors. However, some studies showed that under certain growth conditions the synthesis of Uga4p was not dependent on the presence of γ-aminobutyric acid. To study the effect of the trans-acting factors Uga43p, Uga3p, Uga35p, Ure2p and Gln3p on the expression of UGA4, we measured γ-aminobutyric acid and δ-aminolevulinic acid uptake in yeast mutant cells, lacking one of these regulatory factors, grown under different conditions. Experiments analyzing the UGA4 promoter using a fusion construction UGA4::lacZ were also carried out. The results show that the constitutive expression of the UGA4 gene found in cells under certain growth conditions depends on the presence of Uga3p and Uga35p. In contrast, Gln3p and Ure2p do not seem to have any effect on this constitutive mechanism. Copyright (C) 2000 Federation of European Microbiological Societies.

Registro:

Documento: Artículo
Título:Constitutive expression of the UGA4 gene in Saccharomyces cerevisiae depends on two positive-acting proteins, Uga3p and Uga35p
Autor:Garcia, S.C.; Moretti, M.B.; Batlle, A.
Filiación:Ctro. Invest. sobre Porfirinas y P., FCEyN, UBA), Cd. Univ., Pabellon I., Buenos Aires, Argentina
Palabras clave:γ-Aminobutyric acid; δ-Aminolevulinic acid; Transcriptional factor; Transport regulation; Uga4 permease; Yeast; 4 aminobutyric acid; aminolevulinic acid; permease; article; controlled study; gene expression regulation; nonhuman; priority journal; promoter region; protein transport; Saccharomyces cerevisiae; transcription regulation; Alanine; Aminolevulinic Acid; beta-Galactosidase; DNA-Binding Proteins; Fungal Proteins; GABA Plasma Membrane Transport Proteins; gamma-Aminobutyric Acid; Gene Expression Regulation, Fungal; Membrane Transport Proteins; Organic Anion Transporters; Promoter Regions (Genetics); Recombinant Fusion Proteins; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Transcription Factors; Saccharomyces cerevisiae
Año:2000
Volumen:184
Número:2
Página de inicio:219
Página de fin:224
DOI: http://dx.doi.org/10.1016/S0378-1097(00)00053-7
Título revista:FEMS Microbiology Letters
Título revista abreviado:FEMS Microbiol. Lett.
ISSN:03781097
CODEN:FMLED
CAS:Alanine, 56-41-7; Aminolevulinic Acid, 106-60-5; beta-Galactosidase, EC 3.2.1.23; DAL81 protein, S cerevisiae; DNA-Binding Proteins; Fungal Proteins; GABA permease, 69913-01-5; GABA Plasma Membrane Transport Proteins; gamma-Aminobutyric Acid, 56-12-2; Membrane Transport Proteins; Organic Anion Transporters; Recombinant Fusion Proteins; Saccharomyces cerevisiae Proteins; Transcription Factors; UGA3 protein, S cerevisiae; UGA4 protein, S cerevisiae
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_03781097_v184_n2_p219_Garcia.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v184_n2_p219_Garcia

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Citas:

---------- APA ----------
Garcia, S.C., Moretti, M.B. & Batlle, A. (2000) . Constitutive expression of the UGA4 gene in Saccharomyces cerevisiae depends on two positive-acting proteins, Uga3p and Uga35p. FEMS Microbiology Letters, 184(2), 219-224.
http://dx.doi.org/10.1016/S0378-1097(00)00053-7
---------- CHICAGO ----------
Garcia, S.C., Moretti, M.B., Batlle, A. "Constitutive expression of the UGA4 gene in Saccharomyces cerevisiae depends on two positive-acting proteins, Uga3p and Uga35p" . FEMS Microbiology Letters 184, no. 2 (2000) : 219-224.
http://dx.doi.org/10.1016/S0378-1097(00)00053-7
---------- MLA ----------
Garcia, S.C., Moretti, M.B., Batlle, A. "Constitutive expression of the UGA4 gene in Saccharomyces cerevisiae depends on two positive-acting proteins, Uga3p and Uga35p" . FEMS Microbiology Letters, vol. 184, no. 2, 2000, pp. 219-224.
http://dx.doi.org/10.1016/S0378-1097(00)00053-7
---------- VANCOUVER ----------
Garcia, S.C., Moretti, M.B., Batlle, A. Constitutive expression of the UGA4 gene in Saccharomyces cerevisiae depends on two positive-acting proteins, Uga3p and Uga35p. FEMS Microbiol. Lett. 2000;184(2):219-224.
http://dx.doi.org/10.1016/S0378-1097(00)00053-7