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Abstract:

In order to isolate very strong promoters from bacteria and bacteriophage a plasmid named pProm was constructed. It possesses an origin (ORI) for replication in Gram-negative bacteria, an ORI for replication in Gram-positive bacteria, a promoterless ampicillin resistance gene with a multiple cloning site (MCS) in the position formerly occupied by the ampicillin promoter, a tetracycline resistance gene for selection in Gram-negative bacteria and a chloramphenicol resistance gene for selection in Gram-positive bacteria. Insertion in the MCS of DNA fragments of Staphylococcus aureus bacteriophages resulted in isolation of several clones very resistant to ampicillin. The DNA fragments inserted in these recombinant plasmids were sequenced and all of them contained putative promoter motifs. Direct measurement of the penicillinase activity indicated that one of the isolated promoters could be included within a group of the stronger known prokaryotic promoters. According to these results pProm is a powerful tool to perform studies on promoter strength and for industrial applications. Copyright (C) 1999 Federation of European Microbiological Societies.

Registro:

Documento: Artículo
Título:A plasmid vector for isolation of strong promoters in Escherichia coli
Autor:Carbonelli, D.L.; Corley, E.; Seigelchifer, M.; Zorzópulos, J.
Filiación:Inst. Invest. Biomed. Fund. Pablo C., Saladillo 2452, Buenos Aires, Argentina
Depto. de Quim. Biológica, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Bacterial promoter; Bacteriophage promoter; Promoter isolation; Strong promoter; Vector; ampicillin; DNA fragment; penicillinase; plasmid vector; tetracycline; antibiotic resistance; article; bacteriophage; DNA sequence; Escherichia coli; nonhuman; nucleotide sequence; penicillin resistance; plasmid; priority journal; promoter region; Staphylococcus aureus; Bacteria (microorganisms); bacteriophage a; Escherichia coli; Escherichia coli; Negibacteria; Posibacteria; Prokaryota; Staphylococcus aureus; Staphylococcus aureus; unidentified bacteriophage
Año:1999
Volumen:177
Número:1
Página de inicio:75
Página de fin:82
DOI: http://dx.doi.org/10.1016/S0378-1097(99)00294-3
Título revista:FEMS Microbiology Letters
Título revista abreviado:FEMS Microbiol. Lett.
ISSN:03781097
CODEN:FMLED
CAS:ampicillin, 69-52-3, 69-53-4, 7177-48-2, 74083-13-9, 94586-58-0; penicillinase, 9001-74-5; tetracycline, 23843-90-5, 60-54-8, 64-75-5, 8021-86-1
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_03781097_v177_n1_p75_Carbonelli.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v177_n1_p75_Carbonelli

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Citas:

---------- APA ----------
Carbonelli, D.L., Corley, E., Seigelchifer, M. & Zorzópulos, J. (1999) . A plasmid vector for isolation of strong promoters in Escherichia coli. FEMS Microbiology Letters, 177(1), 75-82.
http://dx.doi.org/10.1016/S0378-1097(99)00294-3
---------- CHICAGO ----------
Carbonelli, D.L., Corley, E., Seigelchifer, M., Zorzópulos, J. "A plasmid vector for isolation of strong promoters in Escherichia coli" . FEMS Microbiology Letters 177, no. 1 (1999) : 75-82.
http://dx.doi.org/10.1016/S0378-1097(99)00294-3
---------- MLA ----------
Carbonelli, D.L., Corley, E., Seigelchifer, M., Zorzópulos, J. "A plasmid vector for isolation of strong promoters in Escherichia coli" . FEMS Microbiology Letters, vol. 177, no. 1, 1999, pp. 75-82.
http://dx.doi.org/10.1016/S0378-1097(99)00294-3
---------- VANCOUVER ----------
Carbonelli, D.L., Corley, E., Seigelchifer, M., Zorzópulos, J. A plasmid vector for isolation of strong promoters in Escherichia coli. FEMS Microbiol. Lett. 1999;177(1):75-82.
http://dx.doi.org/10.1016/S0378-1097(99)00294-3