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Abstract:

The presence of the Φ105cts23 mutant prophage in Bacillus subtilis induces a series of pleiotropic effects that could be ascribed to an anti-SOS activity. In order to circumvent the phage function responsible for this phenomenon, the cts23 mutant repressor was cloned and sequenced. The isolated repressor reduced the survival capacity of the host cells after mitomycin C or nalidixic acid treatments and lowered the spontaneous reversion frequency. When SOS induction kinetics were studied, low or null induction of the damage-inducible din22::LacZ fusion was observed. In contrast, the presence of the wild-type prophage amplified the SOS response. Sequencing of the mutant repressor revealed that the cts23 mutation is a T→C transition affecting the 5' closest codon to one of the two reported DNA binding domains. Copyright (C) 1998 Federation of European Microbiological Societies.

Registro:

Documento: Artículo
Título:Normal induction of the SOS response in Bacillus subtilis is prevented by the mutant repressor from phage φ105cts23
Autor:Rubinstein, C.P.; Guerchicoff, A.; Sanchez-Rivas, C.
Filiación:Fac. de Ciencias Exactas Y Naturales, Depto. Quim. Biol., Cd. Univ., P., 1428 Buenos Aires, Argentina
Palabras clave:Bacillus subtilis; Bacteriophage φ105; Repair; SOS induction; Thermosensitive repressor; article; bacillus subtilis; bacteriophage; dna repair; heat sensitivity; mutant; nonhuman; priority journal; Artificial Gene Fusion; Bacillus Phages; Bacillus subtilis; beta-Galactosidase; DNA Damage; DNA Repair; Gene Expression Regulation, Bacterial; Genes, Viral; Mutation; Plasmids; Repressor Proteins; SOS Response (Genetics); Temperature; Viral Proteins; Bacillus subtilis
Año:1998
Volumen:167
Número:2
Página de inicio:315
Página de fin:320
DOI: http://dx.doi.org/10.1016/S0378-1097(98)00409-1
Título revista:FEMS Microbiology Letters
Título revista abreviado:FEMS Microbiol. Lett.
ISSN:03781097
CODEN:FMLED
CAS:beta-Galactosidase, EC 3.2.1.23; Repressor Proteins; Viral Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v167_n2_p315_Rubinstein

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Citas:

---------- APA ----------
Rubinstein, C.P., Guerchicoff, A. & Sanchez-Rivas, C. (1998) . Normal induction of the SOS response in Bacillus subtilis is prevented by the mutant repressor from phage φ105cts23. FEMS Microbiology Letters, 167(2), 315-320.
http://dx.doi.org/10.1016/S0378-1097(98)00409-1
---------- CHICAGO ----------
Rubinstein, C.P., Guerchicoff, A., Sanchez-Rivas, C. "Normal induction of the SOS response in Bacillus subtilis is prevented by the mutant repressor from phage φ105cts23" . FEMS Microbiology Letters 167, no. 2 (1998) : 315-320.
http://dx.doi.org/10.1016/S0378-1097(98)00409-1
---------- MLA ----------
Rubinstein, C.P., Guerchicoff, A., Sanchez-Rivas, C. "Normal induction of the SOS response in Bacillus subtilis is prevented by the mutant repressor from phage φ105cts23" . FEMS Microbiology Letters, vol. 167, no. 2, 1998, pp. 315-320.
http://dx.doi.org/10.1016/S0378-1097(98)00409-1
---------- VANCOUVER ----------
Rubinstein, C.P., Guerchicoff, A., Sanchez-Rivas, C. Normal induction of the SOS response in Bacillus subtilis is prevented by the mutant repressor from phage φ105cts23. FEMS Microbiol. Lett. 1998;167(2):315-320.
http://dx.doi.org/10.1016/S0378-1097(98)00409-1