Artículo
Pereira, C.M.; Yamauchi, L.M.; Levin, M.J.; Da Silveira, J.F.; Castilho, B.A. "Mapping of B cell epitopes in an immunodominant antigen of Trypanosoma cruzi using fusions to the Escherichia coli LamB protein" (1998) FEMS Microbiology Letters. 164(1):125-131
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Abstract:
The JL8 protein antigen from Trypanosoma cruzi, a dominant immunogen in man, has been characterized as containing tandem amino acid repeats. Here, we describe the use of the Lamb protein of Escherichia coli as a carrier of JL8 derived sequences in order to map the immunodominant B cell epitopes in this antigen. Five different sequences of JL8 were inserted in the Lamb protein and the JL8-LamB fusion proteins were tested by ELISA with human chronic chagasic sera. The fusion carrying the sequence AEKQKAAEATKVAE was recognized by most sera. This protein was also capable of inhibiting the binding of human chagasic antibodies to GST-JL8 in competitive ELISA suggesting that it contains an immunodominant B cell epitope of JL8.
Registro:
| Documento: | Artículo |
| Título: | Mapping of B cell epitopes in an immunodominant antigen of Trypanosoma cruzi using fusions to the Escherichia coli LamB protein |
| Autor: | Pereira, C.M.; Yamauchi, L.M.; Levin, M.J.; Da Silveira, J.F.; Castilho, B.A. |
| Filiación: | Departamento de Microbiologia, Imunologia Parasitol., Esc. P., CEP 04023-062, São Paulo, Brazil Ingebi, 2490 Vuelta del Obrigado, 1428 Buenos Aires, Argentina |
| Palabras clave: | B cell epitope mapping; Chagas disease; Escherichia coli LamB protein; Immunodominant repetitive antigen; Trypanosoma cruzi; bacterial protein; amino acid sequence; antibody response; antigen binding; article; chagas disease; epitope mapping; escherichia coli; immune response; nonhuman; nucleotide sequence; priority journal; serodiagnosis; trypanosoma cruzi; Animals; Antibodies, Bacterial; Antibodies, Protozoan; Antigens, Protozoan; B-Lymphocytes; Bacterial Proteins; Cloning, Molecular; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Epitope Mapping; Epitopes; Escherichia coli; Escherichia coli Proteins; Humans; Immunoblotting; Immunodominant Epitopes; Protein Sorting Signals; Rabbits; Recombinant Fusion Proteins; Trypanosoma cruzi; Bacteria (microorganisms); Escherichia coli; Protozoa; Trypanosoma; Trypanosoma cruzi |
| Año: | 1998 |
| Volumen: | 164 |
| Número: | 1 |
| Página de inicio: | 125 |
| Página de fin: | 131 |
| DOI: | http://dx.doi.org/10.1016/S0378-1097(98)00200-6 |
| Título revista: | FEMS Microbiology Letters |
| Título revista abreviado: | FEMS Microbiol. Lett. |
| ISSN: | 03781097 |
| CODEN: | FMLED |
| CAS: | Antibodies, Bacterial; Antibodies, Protozoan; Antigens, Protozoan; Bacterial Proteins; Epitopes; Escherichia coli Proteins; Immunodominant Epitopes; LamB signal peptide, E coli; Protein Sorting Signals; Recombinant Fusion Proteins |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v164_n1_p125_Pereira |
Referencias:
- Camargo, M.E., An appraisal of Chagas disease serodiagnosis (1992) In: Chagas Disease (American Trypanosomiasis): Its Impact on Transfusion and Clinical Medicine, pp. 165-178. , (Wendel, S., Brener, Z., Camargo, M.E. and Rassi, A., Eds.). International Society of Blood Transfusion, Brazil '92, São Paulo, Brazil
- Franco Da Silveira, J., Trypanosoma cruzi recombinant antigens for serodiagnosis (1992) In: Chagas Disease (American Trypanosomiasis): Its Impact on Transfusion and Clinical Medicine, pp. 225-236. , (Wendel, S., Brener, Z., Camargo, M.E. and Rassi, A., Eds.). International Society of Blood Transfusion, Brazil '92, São Paulo, Brazil
- Ibañez, C.F., Affranchino, J.L., Medina, R.A., Reyes, M.B., Leguizamon, S., Camargo, M.E., Aslund, L., Frasch, A.C.C., Multiple Trypanosoma cruzi antigens containing tandemly repeated amino acid sequence motifs (1988) Mol. Biochem. Parasitol., 30, pp. 27-34
- Lafaille, J.J., Linss, J., Krieger, M.A., Souto-Padrón, T., De Souza, W., Goldenberg, S., Structure and expression of two Trypanosoma cruzi genes encoding antigenic proteins bearing repetitive epitopes (1989) Mol. Biochem. Parasitol., 35, pp. 127-136
- Levin, M.J., Mesri, E.A., Benarous, R., Levitus, G., Schijman, A., Levy-Yeyati, P., Chiale, P., Segura, E.L., Identification of major Trypanosoma cruzi antigenic determinants in chronic Chagas' heart disease (1989) Am. J. Trop. Med. Hyg., 41, pp. 530-539
- Hoft, D.F., Kim, K.S., Otsu, K., Moser, D.R., Yost, W.J., Blumin, J.H., Donelson, J.E., Kirchhoff, L.V., Trypanosoma cruzi expresses diverse repetitive protein antigens (1989) Infect. Immun., 57, pp. 1959-1967
- Levin, M.J., Franco Da Silveira, J., Frasch, A.C.C., Camargo, M.E., Lafon, S., Degrave, W.M., Rangel-Aldao, R., Recombinant Trypanosoma cruzi antigens and Chagas' disease diagnosis: analysis of a workshop (1991) FEMS Microbiol. Immunol., 89, pp. 11-22
- Pastini, A.C., Iglesias, S.R., Carricarte, V.C., Guerin, M.E., Sánchez, D.O., Frasch, A.C., Immunoassay with recombinant Trypanosoma cruzi antigens potentially useful for screening donated blood and diagnosing Chagas disease (1994) Clin. Chem., 40, pp. 1893-1897
- Vergara, U., Lorca, M., Veloso, C., Gonzalez, A., Engstrom, A., Aslund, L., Pettersson, U., Franch, A.C.C., Assay for detection of Trypanosoma cruzi antibodies in human sera based on reaction with synthetic peptides (1991) J. Clin. Microbiol., 29, pp. 2034-2037
- Vergara, U., Veloso, C., Gonzalez, A., Lorca, M., Evaluation of an enzyme-linked immunosorbent assay for the diagnosis of Chagas disease using synthetic peptides (1992) Am. J. Trop. Med. Hyg., 46, pp. 39-43
- Nikado, H., Vaara, M., Molecular basis of bacterial outer membrane permeability (1985) Microbiol. Rev., 49, pp. 1-32
- Boulain, J.C., Charbit, A., Hofnung, M., Mutagenesis by random linker insertion into the lamB gene of Escherichia coli K12 (1986) Mol. Gen. Genet., 205, pp. 339-348
- Charbit, A., Ronco, J., Michel, V., Werts, C., Hofnung, M., Permissive sites and topology of outer membrane protein with a reporter epitope (1991) J. Bacteriol., 173, pp. 262-275
- Smith, D.B., Johnson, K.S., Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase (1988) Gene, 67, pp. 31-40
- Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of the bacteriophage T4 (1970) Nature, 227, pp. 680-685
- Towbin, N., Staehelin, T., Bordon, J., Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets procedure and some applications (1979) Proc. Natl. Acad. Sci. USA, 76, pp. 4350-4354
- Pellequer, L., Westhof, E., Van Regenmortel, M.H.V., Overview of methods for predicting the location of continous epitopes in proteins from their primary structures (1991) Methods Enzymol., 203, pp. 176-201
- Kaplan, D., Ferrari, I., Bergami, P.L., Mahler, E., Levitus, G., Chiale, P., Hoebeke, J., Levin, M.J., Antibodies to ribosomal P proteins of Trypanosoma cruzi in Chagas disease possess functional autoreactivity with heart tissue and differ from anti-P autoantibodies in lupus (1997) Proc. Natl. Acad. Sci. USA, 94, pp. 10301-10306
Citas:
---------- APA ----------
Pereira, C.M., Yamauchi, L.M., Levin, M.J., Da Silveira, J.F. & Castilho, B.A. (1998) . Mapping of B cell epitopes in an immunodominant antigen of Trypanosoma cruzi using fusions to the Escherichia coli LamB protein. FEMS Microbiology Letters, 164(1), 125-131.http://dx.doi.org/10.1016/S0378-1097(98)00200-6
---------- CHICAGO ----------
Pereira, C.M., Yamauchi, L.M., Levin, M.J., Da Silveira, J.F., Castilho, B.A. "Mapping of B cell epitopes in an immunodominant antigen of Trypanosoma cruzi using fusions to the Escherichia coli LamB protein" . FEMS Microbiology Letters 164, no. 1 (1998) : 125-131.http://dx.doi.org/10.1016/S0378-1097(98)00200-6
---------- MLA ----------
Pereira, C.M., Yamauchi, L.M., Levin, M.J., Da Silveira, J.F., Castilho, B.A. "Mapping of B cell epitopes in an immunodominant antigen of Trypanosoma cruzi using fusions to the Escherichia coli LamB protein" . FEMS Microbiology Letters, vol. 164, no. 1, 1998, pp. 125-131.http://dx.doi.org/10.1016/S0378-1097(98)00200-6
---------- VANCOUVER ----------
Pereira, C.M., Yamauchi, L.M., Levin, M.J., Da Silveira, J.F., Castilho, B.A. Mapping of B cell epitopes in an immunodominant antigen of Trypanosoma cruzi using fusions to the Escherichia coli LamB protein. FEMS Microbiol. Lett. 1998;164(1):125-131.http://dx.doi.org/10.1016/S0378-1097(98)00200-6
