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Abstract:

Fusarium oxysporum f. sp. melonis produces extracellular endo-1,4-β-xylanase and β-xylosidase when grown in shaken culture at 26°C in a mineral salts medium containing oat spelt xylan and glucose as carbon sources. Endo-1,4-β-xylanase was purified 251 times from 5-day-old culture filtrates, by Sephacryl S-200, ion exchange and gel filtration HPLC. The purified sample yielded a single band in SDS polyacrylamide gels with a molecular mass of 80 kDa on electrophoretic mobility and 83 kDa by gel filtration behavior. High activity of the endo-1,4-β-xylanase against xylan was observed between 5 and 8 pH, and between 40 and 60°C, the optimum pH and temperature being 5.0 and 50°C, respectively. Kinetic properties of the enzyme are similar to those of other fungal xylanases, showing high affinity towards oat spelt xylan with a Km of 1 mM expressed as xylose equivalent. © 1994.

Registro:

Documento: Artículo
Título:Purification and characterization of an extracellular endo-1,4-β-xylanase from Fusarium oxysporum f. sp. melonis
Autor:Alconada, T.M.; Martínez, M.J.
Filiación:Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, 2 Pabellón, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Centro de Investigaciones Biológicas (CSIC), Departamento de Microbiología Molecular, Velázquez 144, 28006 Madrid, Spain
Palabras clave:Endo-1,4-β-xylanase activity; Fusarium oxysporum f. sp. melonis; β-Xylosidase activity; endo 1,4 beta xylanase; unclassified drug; xylan; article; enzyme activity; enzyme purification; fusarium oxysporum; high performance liquid chromatography; nonhuman; ph; polyacrylamide gel electrophoresis; priority journal; Carbohydrates; Culture Media; Fusarium; Glycoside Hydrolases; Metals; Molecular Weight; Fusarium oxysporum f. sp. melonis; Fusarium sp.; Melonis; Triticum aestivum subsp. spelta
Año:1994
Volumen:118
Número:3
Página de inicio:305
Página de fin:310
Título revista:FEMS Microbiology Letters
Título revista abreviado:FEMS Microbiol. Lett.
ISSN:03781097
CODEN:FMLED
CAS:Carbohydrates; Culture Media; endo-1,4-beta-xylanase, EC 3.2.1.8; Glycoside Hydrolases, EC 3.2.1.; Metals
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v118_n3_p305_Alconada

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  • Martínez, Alconada, Guillén, Vázquez, Reyes, Peptic activities from Fusarium oxysporum f. sp. melonis. Purification and characterization of an exopolygalacturonase (1991) FEMS Microbiol. Lett., 81, pp. 145-150
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Citas:

---------- APA ----------
Alconada, T.M. & Martínez, M.J. (1994) . Purification and characterization of an extracellular endo-1,4-β-xylanase from Fusarium oxysporum f. sp. melonis. FEMS Microbiology Letters, 118(3), 305-310.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v118_n3_p305_Alconada [ ]
---------- CHICAGO ----------
Alconada, T.M., Martínez, M.J. "Purification and characterization of an extracellular endo-1,4-β-xylanase from Fusarium oxysporum f. sp. melonis" . FEMS Microbiology Letters 118, no. 3 (1994) : 305-310.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v118_n3_p305_Alconada [ ]
---------- MLA ----------
Alconada, T.M., Martínez, M.J. "Purification and characterization of an extracellular endo-1,4-β-xylanase from Fusarium oxysporum f. sp. melonis" . FEMS Microbiology Letters, vol. 118, no. 3, 1994, pp. 305-310.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v118_n3_p305_Alconada [ ]
---------- VANCOUVER ----------
Alconada, T.M., Martínez, M.J. Purification and characterization of an extracellular endo-1,4-β-xylanase from Fusarium oxysporum f. sp. melonis. FEMS Microbiol. Lett. 1994;118(3):305-310.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v118_n3_p305_Alconada [ ]