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Abstract:

Elymus scabrifolius is a South American perennial grass exhibiting great potential as a forage resource, especially for environments with edaphic limitations. In the present work we use morphological traits and AFLP molecular markers to genotypically identify six E. scabrifolius accessions, one commercial cultivar and seven artificial hybrids of this species. Both types of marker systems resulted in an accurate differentiation of the interrogated E. scabrifolius germplasm in the dendrograms. Moreover, relationships between the tested germplasm tend to vary according to the type of marker system used. Principal Component Analysis was used to identify the traits that best described the morphological variation. The morphological study revealed that almost all hybrids were different from their parents, with exception of one hybrid that was clustered with their own male parent. In the AFLP marker analysis, all the hybrids were clustered with one of their own parents. However, hybrid origin of those individuals was verified by the presence of paternal bands and polymorphic bands. In conclusion, the use of different methodologies to characterize and analyze E. scabrifolius materials has the potential to become of great benefit for the efficient management of germplasm collections and also for their utilization in breeding programs.

Registro:

Documento: Artículo
Título:Utilization of morphological and AFLP molecular markers in the identification of native and cultivated germplasm of Elymus scabrifolius (Poaceae)
Autor:Tomas, P.A.; Gottlieb, A.M.; Schrauf, G.E.; Poggio, L.
Filiación:Cátedra de Mejoramiento Vegetal y Animal, Universidad Nacional del Litoral, R. P. Kreder 2805, Esperanza, Santa Fe, S3080HOF, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917, Buenos Aires, C1033AAJ, Argentina
Dpto. de Ecología, Genética y Evolución, Universidad de Buenos Aires, Int. Güiraldes 2160. Ciudad Universitaria, Buenos Aires, C1428EGA, Argentina
Universidad de Buenos Aires, Av. San Martín 4453, Buenos Aires, C1417DSE, Argentina
Palabras clave:AFLP; Elymus; Germplasm identification; Molecular markers; Morphology
Año:2013
Volumen:45
Número:2
Página de inicio:85
Página de fin:100
Título revista:Revista de la Facultad de Ciencias Agrarias
Título revista abreviado:Revista Facultad Ciencias Agrarias
ISSN:03704661
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03704661_v45_n2_p85_Tomas

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Citas:

---------- APA ----------
Tomas, P.A., Gottlieb, A.M., Schrauf, G.E. & Poggio, L. (2013) . Utilization of morphological and AFLP molecular markers in the identification of native and cultivated germplasm of Elymus scabrifolius (Poaceae) . Revista de la Facultad de Ciencias Agrarias, 45(2), 85-100.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03704661_v45_n2_p85_Tomas [ ]
---------- CHICAGO ----------
Tomas, P.A., Gottlieb, A.M., Schrauf, G.E., Poggio, L. "Utilization of morphological and AFLP molecular markers in the identification of native and cultivated germplasm of Elymus scabrifolius (Poaceae) " . Revista de la Facultad de Ciencias Agrarias 45, no. 2 (2013) : 85-100.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03704661_v45_n2_p85_Tomas [ ]
---------- MLA ----------
Tomas, P.A., Gottlieb, A.M., Schrauf, G.E., Poggio, L. "Utilization of morphological and AFLP molecular markers in the identification of native and cultivated germplasm of Elymus scabrifolius (Poaceae) " . Revista de la Facultad de Ciencias Agrarias, vol. 45, no. 2, 2013, pp. 85-100.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03704661_v45_n2_p85_Tomas [ ]
---------- VANCOUVER ----------
Tomas, P.A., Gottlieb, A.M., Schrauf, G.E., Poggio, L. Utilization of morphological and AFLP molecular markers in the identification of native and cultivated germplasm of Elymus scabrifolius (Poaceae) . Revista Facultad Ciencias Agrarias. 2013;45(2):85-100.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03704661_v45_n2_p85_Tomas [ ]