Abstract:
An extracellular acid polysaccharide was precipitated at 70% ethanol, together with a mannose-containing carbohydrate, sucrose and glucose, from the culture media of Coelastrum sphaericum. Purification by repeated centrifugation and dialysis and gel permeation cromatography yielded a product composed by an acid polysaccharide together with lesser amounts of a mannose-containing carbohydrate. Methylation analysis showed that the acid polysaccharide had a linear backbone with an average structural unit formed by 3-linked L-rhamnopyranose (2 moles), 3-linked D-galactopyranose (1 mole), 3-linked D-glucopyranose (1 mole) and 4-linked D-glucopyranuronic acid (1 mole). The chain had L-rhamnopyranose (1.9 mol%) and D-galactofuranose (0.5 mol %) single stubs 4-linked to the rhamnopyranose units in the backbone.
Registro:
Documento: |
Artículo
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Título: | An extracellular acid polysaccharide from coelastrum sphaericum |
Autor: | Rodríguez, M.C.; Cerezo, A.S. |
Filiación: | Departamento de Ciencias Biológicas, Ciudad Universitaria, Buenos Aires (1428), Argentina Departamento de Química Orgánica (CIHIDECAR-CONICET), Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Buenos Aires (1428), Argentina
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Año: | 1999
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Volumen: | 87
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Número: | 5-6
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Página de inicio: | 197
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Página de fin: | 203
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Título revista: | Anales des la Asociacion Quimica Argentina
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ISSN: | 03650375
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03650375_v87_n5-6_p197_Rodriguez |
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Citas:
---------- APA ----------
Rodríguez, M.C. & Cerezo, A.S.
(1999)
. An extracellular acid polysaccharide from coelastrum sphaericum. Anales des la Asociacion Quimica Argentina, 87(5-6), 197-203.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03650375_v87_n5-6_p197_Rodriguez [ ]
---------- CHICAGO ----------
Rodríguez, M.C., Cerezo, A.S.
"An extracellular acid polysaccharide from coelastrum sphaericum"
. Anales des la Asociacion Quimica Argentina 87, no. 5-6
(1999) : 197-203.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03650375_v87_n5-6_p197_Rodriguez [ ]
---------- MLA ----------
Rodríguez, M.C., Cerezo, A.S.
"An extracellular acid polysaccharide from coelastrum sphaericum"
. Anales des la Asociacion Quimica Argentina, vol. 87, no. 5-6, 1999, pp. 197-203.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03650375_v87_n5-6_p197_Rodriguez [ ]
---------- VANCOUVER ----------
Rodríguez, M.C., Cerezo, A.S. An extracellular acid polysaccharide from coelastrum sphaericum. 1999;87(5-6):197-203.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03650375_v87_n5-6_p197_Rodriguez [ ]