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Abstract:

An extracellular acid polysaccharide was precipitated at 70% ethanol, together with a mannose-containing carbohydrate, sucrose and glucose, from the culture media of Coelastrum sphaericum. Purification by repeated centrifugation and dialysis and gel permeation cromatography yielded a product composed by an acid polysaccharide together with lesser amounts of a mannose-containing carbohydrate. Methylation analysis showed that the acid polysaccharide had a linear backbone with an average structural unit formed by 3-linked L-rhamnopyranose (2 moles), 3-linked D-galactopyranose (1 mole), 3-linked D-glucopyranose (1 mole) and 4-linked D-glucopyranuronic acid (1 mole). The chain had L-rhamnopyranose (1.9 mol%) and D-galactofuranose (0.5 mol %) single stubs 4-linked to the rhamnopyranose units in the backbone.

Registro:

Documento: Artículo
Título:An extracellular acid polysaccharide from coelastrum sphaericum
Autor:Rodríguez, M.C.; Cerezo, A.S.
Filiación:Departamento de Ciencias Biológicas, Ciudad Universitaria, Buenos Aires (1428), Argentina
Departamento de Química Orgánica (CIHIDECAR-CONICET), Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Buenos Aires (1428), Argentina
Año:1999
Volumen:87
Número:5-6
Página de inicio:197
Página de fin:203
Título revista:Anales des la Asociacion Quimica Argentina
ISSN:03650375
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03650375_v87_n5-6_p197_Rodriguez

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Citas:

---------- APA ----------
Rodríguez, M.C. & Cerezo, A.S. (1999) . An extracellular acid polysaccharide from coelastrum sphaericum. Anales des la Asociacion Quimica Argentina, 87(5-6), 197-203.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03650375_v87_n5-6_p197_Rodriguez [ ]
---------- CHICAGO ----------
Rodríguez, M.C., Cerezo, A.S. "An extracellular acid polysaccharide from coelastrum sphaericum" . Anales des la Asociacion Quimica Argentina 87, no. 5-6 (1999) : 197-203.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03650375_v87_n5-6_p197_Rodriguez [ ]
---------- MLA ----------
Rodríguez, M.C., Cerezo, A.S. "An extracellular acid polysaccharide from coelastrum sphaericum" . Anales des la Asociacion Quimica Argentina, vol. 87, no. 5-6, 1999, pp. 197-203.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03650375_v87_n5-6_p197_Rodriguez [ ]
---------- VANCOUVER ----------
Rodríguez, M.C., Cerezo, A.S. An extracellular acid polysaccharide from coelastrum sphaericum. 1999;87(5-6):197-203.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03650375_v87_n5-6_p197_Rodriguez [ ]