Abstract:
Two isoforms of rat liver Uroporphyrinogen decarboxylase. lf-A (MW 45,000) and If-B (MW 30,000), were purified and characterized. The kinetic value of Km at pH 6.8 -using Uroporphyrinogen III as substrate- was 0.14 μM for If-A, and 0.75 μM for If-B. Using Pentacarboxyporphyrinogen III the values were 1.07 μM for If-A and 1.76 μM for If-B. Besides, If-A was seven times more efficient than If-B in Uroporphyrinogen decarboxylation, whereas If-B did not show great differences in the catalytic efficiency with both substrates. Furthermore, this is the first time that two new isoforms with molecular weights 17,000 and 90,000 were detected.
Registro:
Documento: |
Artículo
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Título: | Detection of four isoforms for rat liver uroporphyrinogen decarboxylase |
Autor: | Ríos De Molina, M.D.C.; Corvi, M.; Chaufan, G.; Guidi, S.; Martín De Viale, L.C.S. |
Filiación: | Laboratorio de Porfirias Experimentales y Metabolismo del Hemo, Departamento de Química Biológica, Universidad de Buenos Aires, CP 1428, Buenos Aires, Argentina
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Año: | 1999
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Volumen: | 87
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Número: | 3-4
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Página de inicio: | 121
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Página de fin: | 125
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Título revista: | Anales des la Asociacion Quimica Argentina
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ISSN: | 03650375
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03650375_v87_n3-4_p121_RiosDeMolina |
Referencias:
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Citas:
---------- APA ----------
Ríos De Molina, M.D.C., Corvi, M., Chaufan, G., Guidi, S. & Martín De Viale, L.C.S.
(1999)
. Detection of four isoforms for rat liver uroporphyrinogen decarboxylase. Anales des la Asociacion Quimica Argentina, 87(3-4), 121-125.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03650375_v87_n3-4_p121_RiosDeMolina [ ]
---------- CHICAGO ----------
Ríos De Molina, M.D.C., Corvi, M., Chaufan, G., Guidi, S., Martín De Viale, L.C.S.
"Detection of four isoforms for rat liver uroporphyrinogen decarboxylase"
. Anales des la Asociacion Quimica Argentina 87, no. 3-4
(1999) : 121-125.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03650375_v87_n3-4_p121_RiosDeMolina [ ]
---------- MLA ----------
Ríos De Molina, M.D.C., Corvi, M., Chaufan, G., Guidi, S., Martín De Viale, L.C.S.
"Detection of four isoforms for rat liver uroporphyrinogen decarboxylase"
. Anales des la Asociacion Quimica Argentina, vol. 87, no. 3-4, 1999, pp. 121-125.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03650375_v87_n3-4_p121_RiosDeMolina [ ]
---------- VANCOUVER ----------
Ríos De Molina, M.D.C., Corvi, M., Chaufan, G., Guidi, S., Martín De Viale, L.C.S. Detection of four isoforms for rat liver uroporphyrinogen decarboxylase. 1999;87(3-4):121-125.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03650375_v87_n3-4_p121_RiosDeMolina [ ]